Intercellular crosstalk in adult dental pulp is mediated by heparin-binding growth factors Pleiotrophin and Midkine
Issued Date
2023-12-01
Resource Type
eISSN
14712164
Scopus ID
2-s2.0-85151837731
Pubmed ID
37024794
Journal Title
BMC Genomics
Volume
24
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
BMC Genomics Vol.24 No.1 (2023)
Suggested Citation
Jiravejchakul N., Abe G.L., Loza M., Park S., Matangkasombut P., Sasaki J.I., Imazato S., Diez D., Standley D.M. Intercellular crosstalk in adult dental pulp is mediated by heparin-binding growth factors Pleiotrophin and Midkine. BMC Genomics Vol.24 No.1 (2023). doi:10.1186/s12864-023-09265-w Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/81366
Title
Intercellular crosstalk in adult dental pulp is mediated by heparin-binding growth factors Pleiotrophin and Midkine
Other Contributor(s)
Abstract
Background: In-depth knowledge of the cellular and molecular composition of dental pulp (DP) and the crosstalk between DP cells that drive tissue homeostasis are not well understood. To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin. Results: Our analysis revealed that DP resident cells have a unique gene expression profile when compared to the reference tissues, and that DP fibroblasts are the main cell type contributing to this expression profile. Genes coding for pleiotrophin (PTN) and midkine (MDK), homologous heparin-binding growth-factors, possessed the highest differential expression levels in DP fibroblasts. In addition, we identified extensive crosstalk between DP fibroblasts and several other DP resident cells, including Schwann cells, mesenchymal stem cells and odontoblasts, mediated by PTN and MDK. Conclusions: DP fibroblasts emerge as unappreciated players in DP homeostasis, mainly through their crosstalk with glial cells. These findings suggest that fibroblast-derived growth factors possess major regulatory functions and thus have a potential role as dental therapeutic targets.