Synthesis of Cationic Quaternized Nanolevan Derivative for Small Molecule and Nucleic Acid Delivery
Issued Date
2023-03-01
Resource Type
eISSN
23102861
Scopus ID
2-s2.0-85151287440
Journal Title
Gels
Volume
9
Issue
3
Rights Holder(s)
SCOPUS
Bibliographic Citation
Gels Vol.9 No.3 (2023)
Suggested Citation
Charoenwongphaibun C., Lorthongpanich C., Septham P., Wangpaiboon K., Panpetch P., Pichyangkura R., Charoenwongpaiboon T., Kuttiyawong K. Synthesis of Cationic Quaternized Nanolevan Derivative for Small Molecule and Nucleic Acid Delivery. Gels Vol.9 No.3 (2023). doi:10.3390/gels9030188 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/81708
Title
Synthesis of Cationic Quaternized Nanolevan Derivative for Small Molecule and Nucleic Acid Delivery
Other Contributor(s)
Abstract
Levan is a biopolymer composed of fructose chains covalently linked by β−2,6 glycosidic linkages. This polymer self−assembles into a nanoparticle of uniform size, making it useful for a wide range of applications. Also, levan exhibits various biological activities such as antioxidants, anti-inflammatory, and anti-tumor, that make this polymer very attractive for biomedical application. In this study, levan synthesized from Erwinia tasmaniensis was chemically modified by glycidyl trimethylammonium chloride (GTMAC) to produce cationized nanolevan (QA-levan). The structure of the obtained GTMAC−modified levan was determined by FT-IR, 1H-NMR and elemental (CHN) analyzer. The size of the nanoparticle was calculated using the dynamic light scattering method (DLS). The formation of DNA/QA-levan polyplex was then investigated by gel electrophoresis. The modified levan was able to increase the solubility of quercetin and curcumin by 11-folds and 205-folds, respectively, compared to free compounds. Cytotoxicity of levan and QA−levan was also investigated in HEK293 cells. This finding suggests that GTMAC−modified levan should have a potential application for drug and nucleic acid delivery.