1-MCP prevents ultrastructural changes in the organelles of Dendrobium petals that are induced by exogenous ethylene
Issued Date
2023-07-01
Resource Type
ISSN
09819428
Scopus ID
2-s2.0-85161043009
Journal Title
Plant Physiology and Biochemistry
Volume
200
Rights Holder(s)
SCOPUS
Bibliographic Citation
Plant Physiology and Biochemistry Vol.200 (2023)
Suggested Citation
Kirasak K., Kunyamee S., Ketsa S. 1-MCP prevents ultrastructural changes in the organelles of Dendrobium petals that are induced by exogenous ethylene. Plant Physiology and Biochemistry Vol.200 (2023). doi:10.1016/j.plaphy.2023.107758 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/83034
Title
1-MCP prevents ultrastructural changes in the organelles of Dendrobium petals that are induced by exogenous ethylene
Author(s)
Author's Affiliation
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Abstract
Ethylene is a plant hormone that causes flower senescence. Dendrobium flowers are sensitive to ethylene and ethylene can induce premature senescence depending on the cultivar and the ethylene concentration. Dendrobium ‘Lucky Duan’ is one of the most sensitive cultivars to ethylene exposure. Open florets of ‘Lucky Duan’ were subjected to ethylene, 1-MCP, or 1-MCP plus ethylene treatments and compared with an untreated control. Ethylene induced earlier development of color fading, drooping and venation in petals, whereas 1-MCP pre-treatment counteracted these changes. Under light microscopy, epidermal cells and mesophyll parenchyma tissue around the vascular bundles of petals treated with ethylene showed collapsed cells whereas 1-MCP pre-treatment counteracted this collapse. An scanning electron microscopy (SEM) study confirmed clearly that ethylene treatment caused the collapse of mesophyll parenchyma tissue around vascular bundles. Ultrastructural changes were also studied using transmission electron microscopy (TEM) and showed that ethylene treatment induced morphological changes in conjunction with disorganization of the plasma membrane, the nuclei, chromatin, the nucleoli, myelin bodies, multivesicular bodies, and mitochondria including changes in size and number, breakages of membranes, enlargement of intercellular spaces and disintegration. 1-MCP pre-treatment was observed to counter these changes that were induced by ethylene. The role of ethylene-induced ultrastructural changes in the different organelles was apparently associated with membrane damage.