Voltage-sensing phosphatase (Vsp) regulates endocytosis-dependent nutrient absorption in chordate enterocytes
Issued Date
2022-12-01
Resource Type
eISSN
23993642
Scopus ID
2-s2.0-85138127125
Pubmed ID
36088390
Journal Title
Communications Biology
Volume
5
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Communications Biology Vol.5 No.1 (2022)
Suggested Citation
Ratanayotha A., Matsuda M., Kimura Y., Takenaga F., Mizuno T., Hossain M.I., Higashijima S.i., Kawai T., Ogasawara M., Okamura Y. Voltage-sensing phosphatase (Vsp) regulates endocytosis-dependent nutrient absorption in chordate enterocytes. Communications Biology Vol.5 No.1 (2022). doi:10.1038/s42003-022-03916-6 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/83081
Title
Voltage-sensing phosphatase (Vsp) regulates endocytosis-dependent nutrient absorption in chordate enterocytes
Other Contributor(s)
Abstract
Voltage-sensing phosphatase (Vsp) is a unique membrane protein that translates membrane electrical activities into the changes of phosphoinositide profiles. Vsp orthologs from various species have been intensively investigated toward their biophysical properties, primarily using a heterologous expression system. In contrast, the physiological role of Vsp in native tissues remains largely unknown. Here we report that zebrafish Vsp (Dr-Vsp), encoded by tpte gene, is functionally expressed on the endomembranes of lysosome-rich enterocytes (LREs) that mediate dietary protein absorption via endocytosis in the zebrafish mid-intestine. Dr-Vsp-deficient LREs were remarkably defective in forming endosomal vacuoles after initial uptake of dextran and mCherry. Dr-Vsp-deficient zebrafish exhibited growth restriction and higher mortality during the critical period when zebrafish larvae rely primarily on exogenous feeding via intestinal absorption. Furthermore, our comparative study on marine invertebrate Ciona intestinalis Vsp (Ci-Vsp) revealed co-expression with endocytosis-associated genes in absorptive epithelial cells of the Ciona digestive tract, corresponding to zebrafish LREs. These findings signify a crucial role of Vsp in regulating endocytosis-dependent nutrient absorption in specialized enterocytes across animal species.