E8002 Reduces Adhesion Formation and Improves Joint Mobility in a Rat Model of Knee Arthrofibrosis
Issued Date
2022-02-01
Resource Type
ISSN
16616596
eISSN
14220067
Scopus ID
2-s2.0-85123117855
Pubmed ID
35163163
Journal Title
International Journal of Molecular Sciences
Volume
23
Issue
3
Rights Holder(s)
SCOPUS
Bibliographic Citation
International Journal of Molecular Sciences Vol.23 No.3 (2022)
Suggested Citation
Takada S., Setoyama K., Norimatsu K., Otsuka S., Nakanishi K., Tani A., Nakakogawa T., Matsuzaki R., Matsuoka T., Sakakima H., Tancharoen S., Maruyama I., Tanaka E., Kikuchi K., Uchikado H. E8002 Reduces Adhesion Formation and Improves Joint Mobility in a Rat Model of Knee Arthrofibrosis. International Journal of Molecular Sciences Vol.23 No.3 (2022). doi:10.3390/ijms23031239 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/84110
Title
E8002 Reduces Adhesion Formation and Improves Joint Mobility in a Rat Model of Knee Arthrofibrosis
Other Contributor(s)
Abstract
Knee arthrofibrosis is a common complication of knee surgery, caused by excessive scar tissue, which results in functional disability. However, no curative treatment has been established. E8002 is an anti-adhesion material that contains L-ascorbic acid, an antioxidant. We aimed to evaluate the efficacy of E8002 for the prevention of knee arthrofibrosis in a rat model, comprising injury to the surface of the femur and quadriceps muscle 1 cm proximal to the patella. Sixteen male, 8week-old Sprague Dawley rats were studied: in the Adhesion group, haemorrhagic injury was induced to the quadriceps and bone, and in the E8002 group, an adhesion-preventing film was implanted between the quadriceps and femur after injury. Six weeks following injury, the restriction of knee flexion owing to fibrotic scarring had not worsened in the E8002 group but had worsened in the Adhesion group. The area of fibrotic scarring was smaller in the E8002 group than in the Adhesion group (p < 0.05). In addition, the numbers of fibroblasts (p < 0.05) and myofibroblasts (p < 0.01) in the fibrotic scar were lower in the E8002 group. Thus, E8002 reduces myofibroblast proliferation and fibrotic scar formation and improves the range of motion of the joint in a model of knee injury.