Apoptotic changes and aquaporin-1 expression in the choroid plexus of cerebral malaria patients
Issued Date
2022-12-01
Resource Type
eISSN
14752875
Scopus ID
2-s2.0-85124636462
Pubmed ID
35151337
Journal Title
Malaria Journal
Volume
21
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Malaria Journal Vol.21 No.1 (2022)
Suggested Citation
Srisook C., Glaharn S., Punsawad C., Viriyavejakul P. Apoptotic changes and aquaporin-1 expression in the choroid plexus of cerebral malaria patients. Malaria Journal Vol.21 No.1 (2022). doi:10.1186/s12936-022-04044-6 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/84865
Title
Apoptotic changes and aquaporin-1 expression in the choroid plexus of cerebral malaria patients
Author(s)
Author's Affiliation
Other Contributor(s)
Abstract
Background: Cerebral malaria (CM) is associated with sequestration of parasitized red blood cells (PRBCs) in the capillaries. Often, the association of CM with cerebral oedema is related with high mortality rate. Morphological changes of the choroid plexus (CP) and caspase-3 expression in CM have not been reported. In addition, limited knowledge is known regarding the role of aquaporin (AQP)-1 in CM. The present study evaluated changes in the CP, explored apoptotic changes and AQP-1 expression in CP epithelial cells (CPECs) in fatal CM patients. Methods: CP from fatal Plasmodium falciparum malaria patients (5 non-CM [NCM], 16 CM) were retrieved and prepared for histopathological evaluation. Caspase-3 and AQP-1 expressions in CPECs were investigated by immunohistochemistry. Results: Histologically, apoptotic changes in CPECs were significantly observed in the CM group compared with the NCM and normal control (NC) groups (p < 0.05). These changes included cytoplasmic and nuclear condensation/shrinkage of CPECs and detachment of CPECs from the basement membrane. The apoptotic changes were positively correlated with caspase-3 expression in the nuclei of CPECs. In addition, AQP-1 expression in CPECs was significantly decreased in the CM group compared with the NCM and NC groups (all p < 0.001). A negative correlation (rs = − 0.450, p = 0.024) was documented between caspase-3 expression in the nuclei of CPECs and AQP-1. Conclusions: Apoptotic changes and altered AQP-1 expression may contribute to CPEC dysfunction and subsequently reduce cerebrospinal fluid production, affecting the water homeostasis in the brains of patients with CM.