Association between Mycobacterium tuberculosis genotype and diabetes mellitus/hypertension: a molecular study
Issued Date
2022-12-01
Resource Type
eISSN
14712334
Scopus ID
2-s2.0-85128801543
Pubmed ID
35462543
Journal Title
BMC Infectious Diseases
Volume
22
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
BMC Infectious Diseases Vol.22 No.1 (2022)
Suggested Citation
Guo S., Lei S., Palittapongarnpim P., McNeil E., Chaiprasert A., Li J., Chen H., Ou W., Surachat K., Qin W., Zhang S., Luo R., Chongsuvivatwong V. Association between Mycobacterium tuberculosis genotype and diabetes mellitus/hypertension: a molecular study. BMC Infectious Diseases Vol.22 No.1 (2022). doi:10.1186/s12879-022-07344-z Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/85322
Title
Association between Mycobacterium tuberculosis genotype and diabetes mellitus/hypertension: a molecular study
Author's Affiliation
Siriraj Hospital
Faculty of Medicine, Prince of Songkia University
Mahidol University
Prince of Songkla University
Thailand National Science and Technology Development Agency
Guiyang Public Health Clinical Center
Liupanshui Center for Disease Control and Prevention
Guizhou Provincial Center for Disease Control and Prevention
Faculty of Medicine, Prince of Songkia University
Mahidol University
Prince of Songkla University
Thailand National Science and Technology Development Agency
Guiyang Public Health Clinical Center
Liupanshui Center for Disease Control and Prevention
Guizhou Provincial Center for Disease Control and Prevention
Other Contributor(s)
Abstract
Background: A paucity of studies focused on the genetic association that tuberculosis (TB) patients with non-communicable diseases (NCDs) are more likely to be infected with Mycobacterium tuberculosis (MTB) with more potent virulence on anti-TB drug resistance than those without NCDs. The study aimed to document the predominant genotype, determine the association between MTB genotypes and NCD status and drug resistance. Methods: We conducted a molecular study in 105 TB patients based on a cross-sectional study focused on the comorbid relationship between chronic conditions and TB among 1773 subjects from September 1, 2019 to August 30, 2020 in Guizhou, China. The participants were investigated through face-to-face interviews, followed by NCDs screening. The DNA of MTB isolates was extracted prior to genotyping using 24 loci MIRU-VNTR. The subsequent evaluations were performed by phylogenetic trees, combined with tests of statistical power, Chi-square or Fisher and multivariate logistic regression analysis. Results: The Beijing family of Lineage 2 (East Asia) was the predominant genotype accounting for 43.8% (46/105), followed by Lineage 4 (Euro-America) strains, including Uganda I (34.3%, 36/105), and the NEW-1 (9.5%, 10/105). The proportion of Beijing strain in patients with and without NCDS was 28.6% (8/28) and 49.4% (38/77), respectively, with a statistical power test value of 24.3%. No significant association was detected between MTB genotype and NCD status. A low clustering rate (2.9%) was identified, consisting of two clusters. The rates of global, mono-, poly- and multi-drug resistance were 16.2% (17/105), 14.3% (15/105), 1.0% (1/105) and 4.8% (5/105), respectively. The drug-resistant rates of rifampicin, isoniazid, and streptomycin, were 6.7% (7/105), 11.4% (12/105) and 5.7% (6/105), respectively. Isoniazid resistance was significantly associated with the Beijing genotype of Lineage 2 (19.6% versus 5.1%). Conclusions: The Lineage 2 East Asia/Beijing genotype is the dominant genotype of the local MTB with endogenous infection preponderating. Not enough evidence is detected to support the association between the MTB genotype and diabetes/hypertension. Isoniazid resistance is associated with the Lineage 2 East Asia/Beijing strain.