Enhancement of the antibiotic activity mediated by the essential oil of Ocotea odorifera (VELL) ROWHER and safrole association
Issued Date
2022-03-01
Resource Type
ISSN
18760341
eISSN
1876035X
Scopus ID
2-s2.0-85117241653
Pubmed ID
34656506
Journal Title
Journal of Infection and Public Health
Volume
15
Issue
3
Start Page
373
End Page
377
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Infection and Public Health Vol.15 No.3 (2022) , 373-377
Suggested Citation
de Almeida R.S. Enhancement of the antibiotic activity mediated by the essential oil of Ocotea odorifera (VELL) ROWHER and safrole association. Journal of Infection and Public Health Vol.15 No.3 (2022) , 373-377. 377. doi:10.1016/j.jiph.2021.09.028 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/86104
Title
Enhancement of the antibiotic activity mediated by the essential oil of Ocotea odorifera (VELL) ROWHER and safrole association
Author(s)
Other Contributor(s)
Abstract
In a recent study, our research group demonstrated that the essential oil of Ocotea odorifera (EOOO) and its major compound safrole potentiated the action fluoroquinolones, modulating bacterial resistance possibly due to direct inhibition of efflux pumps. Thus, in the present study, we investigated whether these treatments could enhance the activity of gentamicin and erythromycin against multidrug-resistant (MDR) bacteria. The EOOO was extracted by hydrodistillation, and the phytochemical analysis was performed by gas chromatography coupled to mass spectrometry (GC–MS). The antibiotic-enhancing effect of the EOOO and safrole against MDR strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa was analyzed by the broth microdilution method. The chemical analysis confirmed the presence of safrole as a major component among the 16 compounds identified in the EOOO. Both the essential oil and the isolated compound showed clinically relevant antibacterial activities against S. aureus. Regarding the modulation of antibiotic resistance, the EOOO was found to enhance the activity of erythromycin against the strains of P. aeruginosa and S. aureus, as well as improving the action of gentamicin against S. aureus. On the other hand, safrole potentiated the activity of gentamicin against the S. aureus strain alone. It is concluded, therefore, that the EOOO and safrole can enhance the activity of macrolides and aminoglycosides, and as such are useful in the development of therapeutic tools to combat bacterial resistance against these classes of antibiotics.