Publication: Genetic loci associated with delayed clearance of Plasmodium falciparum following artemisinin treatment in Southeast Asia.
Issued Date
2013-01-02
Resource Type
Language
eng
ISSN
0027-8424 (printed)
1091-6490 (electronic)
1091-6490 (electronic)
Rights
Mahidol University
Rights Holder(s)
PubMed Central
Bibliographic Citation
Takala-Harrison S, Clark TG, Jacob CG, Cummings MP, Miotto O, Dondorp AM. et al. Genetic loci associated with delayed clearance of Plasmodium falciparum following artemisinin treatment in Southeast Asia. Proc Natl Acad Sci U S A. 2013 Jan 2;110(1):240-5.
Suggested Citation
Takala-Harrisona, Shannon, Clark, Taane G., Cummings, Michael P., Miotto,Olivo, Dondorp, Arjen M., Fukudaf, Mark M., Nosten, Francois, Noedl, Harald, Mallika Imwong, มัลลิกา อิ่มวงศ์, Bethell, Delia, Se, Youry, Lon, Chanthap, Tyner, Stuart D., Saunders, David L., Socheat, Duong, Ariey, Frederic, Phyo, Aung Pyae, Starzengruber, Peter, Fuehrer, Hans-Peter, Swoboda, Paul, Stepniewska, Kasia, Flegg, Jennifer, Arze, Cesar, Cerqueira, Gustavo C., Silva, Joana C., Ricklefs, Stacy M., Porcella, Stephen F., Stephens, Robert M., Adams, Matthew, Kenefic, Leo J., Campino, Susana, Auburn, Sarah, MacInnis, Bronwyn, Kwiatkowski, Dominic P., Su, Xin-zhuan, White, Nicholas J., Ringwald, Pascal, Plowe, Christopher V. Genetic loci associated with delayed clearance of Plasmodium falciparum following artemisinin treatment in Southeast Asia.. Takala-Harrison S, Clark TG, Jacob CG, Cummings MP, Miotto O, Dondorp AM. et al. Genetic loci associated with delayed clearance of Plasmodium falciparum following artemisinin treatment in Southeast Asia. Proc Natl Acad Sci U S A. 2013 Jan 2;110(1):240-5.. doi:10.1073/pnas.1211205110. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/862
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Genetic loci associated with delayed clearance of Plasmodium falciparum following artemisinin treatment in Southeast Asia.
Author(s)
Takala-Harrisona, Shannon
Clark, Taane G.
Cummings, Michael P.
Miotto,Olivo
Dondorp, Arjen M.
Fukudaf, Mark M.
Nosten, Francois
Noedl, Harald
Mallika Imwong
มัลลิกา อิ่มวงศ์
Bethell, Delia
Se, Youry
Lon, Chanthap
Tyner, Stuart D.
Saunders, David L.
Socheat, Duong
Ariey, Frederic
Phyo, Aung Pyae
Starzengruber, Peter
Fuehrer, Hans-Peter
Swoboda, Paul
Stepniewska, Kasia
Flegg, Jennifer
Arze, Cesar
Cerqueira, Gustavo C.
Silva, Joana C.
Ricklefs, Stacy M.
Porcella, Stephen F.
Stephens, Robert M.
Adams, Matthew
Kenefic, Leo J.
Campino, Susana
Auburn, Sarah
MacInnis, Bronwyn
Kwiatkowski, Dominic P.
Su, Xin-zhuan
White, Nicholas J.
Ringwald, Pascal
Plowe, Christopher V.
Clark, Taane G.
Cummings, Michael P.
Miotto,Olivo
Dondorp, Arjen M.
Fukudaf, Mark M.
Nosten, Francois
Noedl, Harald
Mallika Imwong
มัลลิกา อิ่มวงศ์
Bethell, Delia
Se, Youry
Lon, Chanthap
Tyner, Stuart D.
Saunders, David L.
Socheat, Duong
Ariey, Frederic
Phyo, Aung Pyae
Starzengruber, Peter
Fuehrer, Hans-Peter
Swoboda, Paul
Stepniewska, Kasia
Flegg, Jennifer
Arze, Cesar
Cerqueira, Gustavo C.
Silva, Joana C.
Ricklefs, Stacy M.
Porcella, Stephen F.
Stephens, Robert M.
Adams, Matthew
Kenefic, Leo J.
Campino, Susana
Auburn, Sarah
MacInnis, Bronwyn
Kwiatkowski, Dominic P.
Su, Xin-zhuan
White, Nicholas J.
Ringwald, Pascal
Plowe, Christopher V.
Corresponding Author(s)
Abstract
The recent emergence of artemisinin-resistant Plasmodium falciparum malaria in
western Cambodia could threaten prospects for malaria elimination. Identification
of the genetic basis of resistance would provide tools for molecular
surveillance, aiding efforts to contain resistance. Clinical trials of artesunate
efficacy were conducted in Bangladesh, in northwestern Thailand near the Myanmar
border, and at two sites in western Cambodia. Parasites collected from trial
participants were genotyped at 8,079 single nucleotide polymorphisms (SNPs) using
a P. falciparum-specific SNP array. Parasite genotypes were examined for
signatures of recent positive selection and association with parasite clearance
phenotypes to identify regions of the genome associated with artemisinin
resistance. Four SNPs on chromosomes 10 (one), 13 (two), and 14 (one) were
significantly associated with delayed parasite clearance. The two SNPs on
chromosome 13 are in a region of the genome that appears to be under strong
recent positive selection in Cambodia. The SNPs on chromosomes 10 and 13 lie in
or near genes involved in postreplication repair, a DNA damage-tolerance pathway.
Replication and validation studies are needed to refine the location of loci
responsible for artemisinin resistance and to understand the mechanism behind it;
however, two SNPs on chromosomes 10 and 13 may be useful markers of delayed
parasite clearance in surveillance for artemisinin resistance in Southeast Asia.