Magnetic Graphene Oxide Nanocomposites for Selective miRNA Separation and Recovery
Issued Date
2023-01-01
Resource Type
eISSN
24701343
Scopus ID
2-s2.0-85181816270
Journal Title
ACS Omega
Rights Holder(s)
SCOPUS
Bibliographic Citation
ACS Omega (2023)
Suggested Citation
Uten S., Boonbanjong P., Prueksathaporn Y., Treerattrakoon K., Sathirapongsasuti N., Chanlek N., Pinitsoontorn S., Luksirikul P., Japrung D. Magnetic Graphene Oxide Nanocomposites for Selective miRNA Separation and Recovery. ACS Omega (2023). doi:10.1021/acsomega.3c05919 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/95723
Title
Magnetic Graphene Oxide Nanocomposites for Selective miRNA Separation and Recovery
Corresponding Author(s)
Other Contributor(s)
Abstract
In this study, we developed magnetic graphene oxide composites by chemically attaching Fe3O4 nanoparticles to graphene oxide nanosheets. Characterization techniques, including Fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), Raman spectroscopy, thermogravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and transmission electron microscopy (TEM), confirmed the successful synthesis of Fe3O4@GO composites with desirable properties. The resulting composites exhibited superparamagnetic behavior, solubility, and compatibility for efficient miRNA separation. Using miR-29a as a model, we demonstrated the effective binding of miR-29a to the magnetic graphene oxide (GO) composites at an optimal concentration of 1.5 mg/mL, followed by a simple separation using magnetic forces. Additionally, the addition of 5.0 M urea enhanced the miRNA recovery. These findings highlight the potential use of our magnetic graphene oxide composites for the efficient separation and recovery of miR-29a, suggesting their broad applicability in various miRNA-based studies. Further exploration can focus on investigating endogenous miRNAs with aberrant expression patterns, contributing to the advancements in precision medicine.