Layering vaccination with antibiotic therapy results in protection and clearance of Burkholderia pseudomallei in Balb/c mice
Issued Date
2024-03-01
Resource Type
ISSN
00199567
eISSN
10985522
Scopus ID
2-s2.0-85187718911
Pubmed ID
38289122
Journal Title
Infection and Immunity
Volume
92
Issue
3
Rights Holder(s)
SCOPUS
Bibliographic Citation
Infection and Immunity Vol.92 No.3 (2024)
Suggested Citation
Barnes K.B., Brett P., Burtnick M., Vente A., Bentley C., Richards M.I., Flick-Smith H.C., Burgess G., Thwaite J.E., Laws T.R., Maishman T.C., Nelson M., Harding S.V. Layering vaccination with antibiotic therapy results in protection and clearance of Burkholderia pseudomallei in Balb/c mice. Infection and Immunity Vol.92 No.3 (2024). doi:10.1128/iai.00455-23 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/97709
Title
Layering vaccination with antibiotic therapy results in protection and clearance of Burkholderia pseudomallei in Balb/c mice
Corresponding Author(s)
Other Contributor(s)
Abstract
Melioidosis is a disease that is difficult to treat due to the causative organism, Burkholderia pseudomallei being inherently antibiotic resistant and it having the ability to invade, survive, and replicate in an intracellular environment. Combination therapy approaches are routinely being evaluated in animal models with the aim of improving the level of protection and clearance of colonizing bacteria detected. In this study, a subunit vaccine layered with the antibiotic finafloxacin was evaluated in vivo against an inhalational infection with B. pseudomallei in Balb/c mice. Groups of mice vaccinated, infected, and euthanized at antibiotic initiation had a reduced bacterial load compared to those that had not been immunized. In addition, the subunit vaccine provided a synergistic effect when it was delivered with a CpG ODN and finafloxacin was initiated at 48 h post-challenge. Vaccination was also shown to improve the outcome, in a composite measure of survival and clearance. In summary, layering a subunit vaccine with the antibiotic finafloxacin is a promising therapeutic alternative for use in the treatment of B. pseudomallei infections.