Antimicrobial and antioxidant activities of lignin by-product from sugarcane leaf conversion to levulinic acid and hydrochar
Issued Date
2024-07-01
Resource Type
eISSN
22149937
Scopus ID
2-s2.0-85192832262
Journal Title
Sustainable Materials and Technologies
Volume
40
Rights Holder(s)
SCOPUS
Bibliographic Citation
Sustainable Materials and Technologies Vol.40 (2024)
Suggested Citation
Nakason K., Chukaew P., Utrarachkij F., Kuboon S., Kraithong W., Pichaiyut S., Wanmolee W., Panyapinyopol B. Antimicrobial and antioxidant activities of lignin by-product from sugarcane leaf conversion to levulinic acid and hydrochar. Sustainable Materials and Technologies Vol.40 (2024). doi:10.1016/j.susmat.2024.e00973 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/98381
Title
Antimicrobial and antioxidant activities of lignin by-product from sugarcane leaf conversion to levulinic acid and hydrochar
Corresponding Author(s)
Other Contributor(s)
Abstract
To enhance the sustainability of lignocellulosic biomass utilization process, a significant attention is given to a high-value lignin byproduct of this process. Herein, antimicrobial, antioxidant, and physicochemical properties of lignin in black liquor extracted from sugarcane leaf (SCL) for levulinic acid (LA) and hydrochar production were investigated. The lignin was extracted from SCL through an alkaline pretreatment process using 5–25 wt% of either NaOH or KOH at 120–160 °C. Disk diffusion susceptibility tests, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) revealed that the SCL lignin is effective against Gram-positive bacteria (S. aureus) rather than Gram-negative bacteria (E. coli and S. typhimurium). Different alkaline reagents marginally affected the antimicrobial and antioxidant activities of the lignin. Py-GC/MS analysis results determined that the SCL lignin contained mainly p-hydroxyphenyl (H) and guaiacyl (G) with a small amount of syringyl (S) lignin. Furthermore, the structural alterations and linkages of SCL lignin during alkaline pretreatment were examined using 2D-HSQC NMR, providing valuable insights into the transformation processes. The conversion of delignified SCL to LA through a catalytic hydrothermal process using various acid catalysts indicated that the alkaline pretreatment could enhance LA yield with a maximum yield of 33.4 wt%. Additionally, fuel property characterization of the hydrochar co-product from LA production determined that the hydrochar can be used as a coal substitute.