Semi-quantitative analysis of formaldehyde in food using calibration chart based on number of colored wells of microwell plate titration
Issued Date
2024-10-30
Resource Type
eISSN
25901575
Scopus ID
2-s2.0-85197505683
Journal Title
Food Chemistry: X
Volume
23
Rights Holder(s)
SCOPUS
Bibliographic Citation
Food Chemistry: X Vol.23 (2024)
Suggested Citation
Tongdee M., Wilairat P., Praditweangkum W., Chantiwas R. Semi-quantitative analysis of formaldehyde in food using calibration chart based on number of colored wells of microwell plate titration. Food Chemistry: X Vol.23 (2024). doi:10.1016/j.fochx.2024.101617 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/99618
Title
Semi-quantitative analysis of formaldehyde in food using calibration chart based on number of colored wells of microwell plate titration
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Abstract
Microplate titration quantifies sodium hydroxide generated from formaldehyde reacting with excess sulfite in a 96-microwell plate. Phenolphthalein indicators change from red to colorless when all hydroxide ions react. Methodology optimized reagent concentrations, and reaction time and created a Calibration Chart for semi-quantitative determination. The chart shows formaldehyde concentration ranges corresponding to red well counts from 0 to 200 mM in 20 mM increments. Inter-operator repeatability demonstrates precision (3 replicates), correlating red wells with standard formaldehyde concentrations. This instrument-free technique uses readily available commercial plates, eliminating the need for specialized equipment and calibration. The methodology offers simplicity with its reliance on readily available commercial plates and minimal specialized equipment, hence it is cost-effective and easily transportable 96-microwell plates enhancing the methodology's portability, and efficient semi-quantitative analysis of formaldehyde. The analysis of twelve solutions from food samples agrees with the quantitative values using titration.