Bacteriophages isolated from mouse feces attenuates pneumonia mice caused by Pseudomonas aeruginosa
Issued Date
2024-07-01
Resource Type
eISSN
19326203
Scopus ID
2-s2.0-85198722898
Journal Title
PLoS ONE
Volume
19
Issue
7 July
Rights Holder(s)
SCOPUS
Bibliographic Citation
PLoS ONE Vol.19 No.7 July (2024)
Suggested Citation
Sutnu N., Chancharoenthana W., Kamolratanakul S., Phuengmaung P., Singkham-In U., Chongrak C., Montathip S., Wannigama D.L., Chatsuwan T., Ounjai P., Schultz M.J., Leelahavanichkul A. Bacteriophages isolated from mouse feces attenuates pneumonia mice caused by Pseudomonas aeruginosa. PLoS ONE Vol.19 No.7 July (2024). doi:10.1371/journal.pone.0307079 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/99774
Title
Bacteriophages isolated from mouse feces attenuates pneumonia mice caused by Pseudomonas aeruginosa
Author's Affiliation
Faculty of Tropical Medicine, Mahidol University
UWA Medical School
Mahidol Oxford Tropical Medicine Research Unit
Rangsit University
Mahidol University
Nuffield Department of Medicine
Faculty of Medicine, Chulalongkorn University
Universiteit van Amsterdam
The University of Sheffield
Yamakata Prefectural Central Hospital
UWA Medical School
Mahidol Oxford Tropical Medicine Research Unit
Rangsit University
Mahidol University
Nuffield Department of Medicine
Faculty of Medicine, Chulalongkorn University
Universiteit van Amsterdam
The University of Sheffield
Yamakata Prefectural Central Hospital
Corresponding Author(s)
Other Contributor(s)
Abstract
Background Most of the current bacteriophages (phages) are mostly isolated from environments. However, phages isolated from feces might be more specific to the bacteria that are harmful to the host. Meanwhile, some phages from the environment might affect non-pathogenic bacteria for the host. Methods Here, bacteriophages isolated from mouse feces were intratracheally (IT) or intravenously (IV) administered in pneumonia mice caused by Pseudomonas aeruginosa at 2 hours postintratracheal bacterial administration. As such, the mice with phage treatment, using either IT or IV administration, demonstrated less severe pneumonia as indicated by mortality, serum cytokines, bacteremia, bacterial abundance in bronchoalveolar lavage fluid (BALF), and neutrophil extracellular traps (NETs) in lung tissue (immunofluorescence of neutrophil elastase and myeloperoxidase). Results Interestingly, the abundance of phages in BALF from the IT and IV injections was similar, supporting a flexible route of phage administration. With the incubation of bacteria with neutrophils, the presence of bacteriophages significantly improved bactericidal activity, but not NETs formation, with the elevated supernatant IL-6 and TNF-α, but not IL-1β. In conclusion, our findings suggest that bacteriophages against Pseudomonas aeruginosa can be discovered from feces of the host. Conclusions The phages attenuate pneumonia partly through an enhanced neutrophil bactericidal activity, but not via inducing NETs formation. The isolation of phages from the infected hosts themselves might be practically useful for future treatment. More studies are warranted.