Generation of RUNX1c-eGFP induced pluripotent stem cell, MUSIi012-A-4, using CRISPR/Cas9
Issued Date
2023-03-01
Resource Type
ISSN
18735061
eISSN
18767753
Scopus ID
2-s2.0-85147360845
Pubmed ID
36753833
Journal Title
Stem Cell Research
Volume
67
Rights Holder(s)
SCOPUS
Bibliographic Citation
Stem Cell Research Vol.67 (2023)
Suggested Citation
Srisook P., Laowtammathron C., Lorthongpanich C., Klaihmon P., Terbto P., Waeteekul S., U-pratya Y., Issaragrisil S. Generation of RUNX1c-eGFP induced pluripotent stem cell, MUSIi012-A-4, using CRISPR/Cas9. Stem Cell Research Vol.67 (2023). doi:10.1016/j.scr.2023.103035 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/81645
Title
Generation of RUNX1c-eGFP induced pluripotent stem cell, MUSIi012-A-4, using CRISPR/Cas9
Author's Affiliation
Other Contributor(s)
Abstract
Runt-Related Transcription Factor 1c (RUNX1c) plays an important role in regulating the development of hematopoietic stem cells (HSC). Using CRISPR/Cas9 gene editing technology, we established a RUNX1c-eGFP reporter cell line from the MUSIi012-A cell line. The MUSIi012-A-4 cell line has normal stem cell morphology and karyotype, expresses pluripotency markers, and can be differentiated into all three germ layers in vitro and in vivo. This cell line serves as a valuable model to observe the expression of RUNX1c via eGFP tracking during human hematopoietic development.