Generation of RUNX1c-eGFP induced pluripotent stem cell, MUSIi012-A-4, using CRISPR/Cas9

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dc.contributor.authorSrisook P.
dc.contributor.authorLaowtammathron C.
dc.contributor.authorLorthongpanich C.
dc.contributor.authorKlaihmon P.
dc.contributor.authorTerbto P.
dc.contributor.authorWaeteekul S.
dc.contributor.authorU-pratya Y.
dc.contributor.authorIssaragrisil S.
dc.contributor.otherMahidol University
dc.date.accessioned2023-05-19T07:35:23Z
dc.date.available2023-05-19T07:35:23Z
dc.date.issued2023-03-01
dc.description.abstractRunt-Related Transcription Factor 1c (RUNX1c) plays an important role in regulating the development of hematopoietic stem cells (HSC). Using CRISPR/Cas9 gene editing technology, we established a RUNX1c-eGFP reporter cell line from the MUSIi012-A cell line. The MUSIi012-A-4 cell line has normal stem cell morphology and karyotype, expresses pluripotency markers, and can be differentiated into all three germ layers in vitro and in vivo. This cell line serves as a valuable model to observe the expression of RUNX1c via eGFP tracking during human hematopoietic development.
dc.identifier.citationStem Cell Research Vol.67 (2023)
dc.identifier.doi10.1016/j.scr.2023.103035
dc.identifier.eissn18767753
dc.identifier.issn18735061
dc.identifier.pmid36753833
dc.identifier.scopus2-s2.0-85147360845
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/81645
dc.rights.holderSCOPUS
dc.subjectBiochemistry, Genetics and Molecular Biology
dc.titleGeneration of RUNX1c-eGFP induced pluripotent stem cell, MUSIi012-A-4, using CRISPR/Cas9
dc.typeArticle
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85147360845&origin=inward
oaire.citation.titleStem Cell Research
oaire.citation.volume67
oairecerif.author.affiliationSiriraj Hospital
oairecerif.author.affiliationWattanosoth Hospital

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