Isolation and identification of antimalarial substances from streptomyces culture broth
Issued Date
2024
Copyright Date
1993
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
xiv, 121 leaves : ill. (some col.)
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Microbiology))--Mahidol University, 1993
Suggested Citation
Pattra Suntornthiticharoen Isolation and identification of antimalarial substances from streptomyces culture broth. Thesis (M.Sc. (Microbiology))--Mahidol University, 1993. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/99956
Title
Isolation and identification of antimalarial substances from streptomyces culture broth
Alternative Title(s)
การแยกและวิเคราะห์สารที่มีฤทธิ์ฆ่ามาลาเรียจากน้ำเลี้ยงเชื้อ streptomyces
Author(s)
Abstract
Streptomyces sp. var. 245.1, a new isolate of Streptomyces from soil collected from Petchaboon province (northern Thailand), was used as a producer of an antimalarial substance. The antimalarial activity was determined by using the in vitro microtest technique. The crude culture broth actively inhibited the development of Plasmodium falciparum at an MIC of 1 ug/ml. It was extracted with dichloromethane and ethyl acetate consecutively. The extract was active at an MIC of 0.1 ug/ml. Cultivation of the organism was done in both small and large scales. The antimalarial substance(s) from small scale cultivation was extracted with dichloromethane and ethyl acetate and then partially purified-by preparative thin-layer chromatography (PLC). The fractions named PLC 4/S and PLC 5/S were found to possess high antimalarial activity with an MIC of 10 ng/ml. Antimalarial substance(s) from large scale cultivation was firstly extracted with dichloromethane, ethyl acetate and followed by hexane in order to get rid of antifoam. The antimalarial substance(s) was found mainly in hexane insoluble part. PLC separation of the hexane insoluble part yielded 5 prominent fractions. One of the fractions, PLC 5/F, showed very high antimalarial activity with an MIC of 1 ng/ml. It was further used for physical and chemical analyses (NMR, IR).
Description
Microbiology (Mahidol University 1993)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Microbiology
Degree Grantor(s)
Mahidol University