Development of Campylobacter cultivation system using oxygen-reducing membrane fragment
1
Issued Date
2009
Copyright Date
2009
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
xii, 89 leaves : ill.
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Medical Technology))--Mahidol University, 2009
Suggested Citation
Pattara Wongjaroen Development of Campylobacter cultivation system using oxygen-reducing membrane fragment. Thesis (M.Sc. (Medical Technology))--Mahidol University, 2009. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/96017
Title
Development of Campylobacter cultivation system using oxygen-reducing membrane fragment
Alternative Title(s)
การพัฒนาระบบการเพาะเลี้ยงเชื้อ Campylobacter โดยใช้ Oxygen-reducing membrane fragment
Author(s)
Advisor(s)
Abstract
The purpose of this study is to develop a Campylobacter cultivation method under normal atmosphere by using oxygen-reducing membrane fragments. Membrane fragments were extracted from Escherichia coli and Pseudomonas aeruginosa isolates. The isolates with the highest efficiency of oxygen reduction were selected to obtain purified membrane fragments. The purified membrane fragments were characterized and investigated for supporting Campylobacter jejuni growth in broth. Appropriated concentration of membrane fragments were choosen for formulating the developed culture model of agar-type media. The culture model was evaluated by culturing Campylobacter from pure culture, artificially inoculated and contaminated chicken samples. Purified membrane fragments from E. coli showed good oxygen reduction at 42°C and 50 °C, and at pH 7.5 to pH 8.5, while membrane fragments from P. aeruginosa worked well at 37°C and 42°C, and at pH 7.0 to pH 7.5. Campylobacter growth in the broth containing purified membrane fragments could be observed within 6 to 12 hours of incubation. Using 0.025 U of membrane fragments in the developed culture model could support Campylobacter growth from pure culture, artificially inoculated and contaminated chicken samples. Using developed culture model with oxygen-reducing membrane fragments demonstrates a possibility for substitution of the conventional method.
Description
Medical Technology (Mahidol University 2009)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Medical Technology
Degree Discipline
Medical Technology
Degree Grantor(s)
Mahidol University