Binding properties of spectrin to thalassemic red cell membranes
1
Issued Date
2024
Copyright Date
1989
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
xii, 131 leaves : ill.
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Biochemistry))--Mahidol University, 1989
Suggested Citation
Aranya Kittikalayawong Binding properties of spectrin to thalassemic red cell membranes. Thesis (M.Sc. (Biochemistry))--Mahidol University, 1989. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/101014
Title
Binding properties of spectrin to thalassemic red cell membranes
Alternative Title(s)
การศึกษาการยึดเกาะของโปรตีนสเปคตรินบนเม็ดเลือดแดงในโรคธาลัสซีเมีย
Author(s)
Advisor(s)
Abstract
The underlying cause of pathology in thalassemia is the premature destruction of erythrocytes both in the bone marrow and the reticuloendothelial (RE) system. This is believed to result from oxidative stress caused by excess globin chains lying at the cytosolic face of the membrane. Binding study of normal spectrin to spectrin-depleted red cell vesicles was performed to investigate the properties of red cell membrane in a group of 3 patients with Hb H disease, Hb H in conjunction with Hb Constant Spring and beta-thalassemia/Hb E disease, together with 6 control normal subjects. Thalassemic red cells had the same ability to form vesicles as control and the protein compositions as analyzed on sodium dodecyl-sulfate polyacrylamide gel electrophoresis were also similar. Normal red cell vesicles showed a binding capacity of 96±10 ug/mg membrane protein with an affinity of 6.0±2.0 ug/ml or 12.9±4.4 nM. No differences in these parameters were detected between the thalassemia calls and control. It is concluded that specific binding site of ankyrin for spectrin in thalassemic red cell has not been affected by the presence of excess chains.
Description
Biochemistry (Mahidol University 1989)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Biochemistry
Degree Grantor(s)
Mahidol University