A novel single-step low-volume solvent extraction method for quantification of bioactive compounds in different rice bran varieties
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Issued Date
2026-05-01
Resource Type
ISSN
08891575
eISSN
10960481
Scopus ID
2-s2.0-105034806877
Journal Title
Journal of Food Composition and Analysis
Volume
153
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SCOPUS
Bibliographic Citation
Journal of Food Composition and Analysis Vol.153 (2026)
Suggested Citation
Manakla S., Premkamon P., Lilitchan S. A novel single-step low-volume solvent extraction method for quantification of bioactive compounds in different rice bran varieties. Journal of Food Composition and Analysis Vol.153 (2026). doi:10.1016/j.jfca.2026.109127 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/116132
Title
A novel single-step low-volume solvent extraction method for quantification of bioactive compounds in different rice bran varieties
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Abstract
AbstractA novel single-step low-volume solvent extraction (SLSE) method was developed to quantify γ-oryzanol and ferulic acid in rice bran, reducing solvent consumption and processing time compared with conventional multi-step methods. Once the adsorption coefficients (K<inf>d</inf>) were determined, rapid and accurate quantification and prediction of extractability were enabled. At 30 °C, the K<inf>d</inf> values obtained with methanol were 2.34 for ferulic acid and 1.41 for γ-oryzanol in rice bran, and 2.26 and 1.66 in defatted rice bran. The SLSE method showed good agreement with the partial extraction method, with maximum deviations of 5.09% for ferulic acid and 5.65% for γ-oryzanol (p > 0.05). Based on the K<inf>d</inf> values, 90.0–90.4% of ferulic acid and 84.9–86.9% of γ-oryzanol were extracted at a solvent-to-bran ratio of 4:1, while 94.8–94.9% of ferulic acid and 91.9–93.0% of γ-oryzanol were extracted at 8:1, consistent with the experimental results. The proposed method provides a convenient, economical, and environmentally friendly approach for routine analysis, with potential application to other plant-derived compounds.