SaSi qPCR: a novel duplex qPCR assay for rapid and simultaneous detection of Streptococcus agalactiae and Streptococcus iniae in fish
1
Issued Date
2026-12-01
Resource Type
eISSN
17560500
Scopus ID
2-s2.0-105026893243
Pubmed ID
41327378
Journal Title
BMC Research Notes
Volume
19
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
BMC Research Notes Vol.19 No.1 (2026)
Suggested Citation
Taengphu S., Dong H.T., Meemetta W., Senapin S. SaSi qPCR: a novel duplex qPCR assay for rapid and simultaneous detection of Streptococcus agalactiae and Streptococcus iniae in fish. BMC Research Notes Vol.19 No.1 (2026). doi:10.1186/s13104-025-07577-6 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/114756
Title
SaSi qPCR: a novel duplex qPCR assay for rapid and simultaneous detection of Streptococcus agalactiae and Streptococcus iniae in fish
Author(s)
Corresponding Author(s)
Other Contributor(s)
Abstract
Objective: To develop, optimize, and validate a novel duplex quantitative PCR (qPCR) assay, termed SaSi qPCR, for the simultaneous detection of Streptococcus agalactiae (Sa) and Streptococcus iniae (Si) in fish samples. Results: The SaSi qPCR assay successfully detected DNA from bacterial cultures and fish specimens, including tilapia (Oreochromis sp.) and Asian sea bass (Lates calcarifer). The method achieved a detection limit of 20 copies per reaction for both targets, with no cross-reactivity observed with other tested fish pathogens. The assay was applied to clinical fish specimens and detected S. agalactiae, S. iniae, and co-infections in fish tissues.