SaSi qPCR: a novel duplex qPCR assay for rapid and simultaneous detection of Streptococcus agalactiae and Streptococcus iniae in fish

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dc.contributor.authorTaengphu S.
dc.contributor.authorDong H.T.
dc.contributor.authorMeemetta W.
dc.contributor.authorSenapin S.
dc.contributor.correspondenceTaengphu S.
dc.contributor.otherMahidol University
dc.date.accessioned2026-02-06T18:31:50Z
dc.date.available2026-02-06T18:31:50Z
dc.date.issued2026-12-01
dc.description.abstractObjective: To develop, optimize, and validate a novel duplex quantitative PCR (qPCR) assay, termed SaSi qPCR, for the simultaneous detection of Streptococcus agalactiae (Sa) and Streptococcus iniae (Si) in fish samples. Results: The SaSi qPCR assay successfully detected DNA from bacterial cultures and fish specimens, including tilapia (Oreochromis sp.) and Asian sea bass (Lates calcarifer). The method achieved a detection limit of 20 copies per reaction for both targets, with no cross-reactivity observed with other tested fish pathogens. The assay was applied to clinical fish specimens and detected S. agalactiae, S. iniae, and co-infections in fish tissues.
dc.identifier.citationBMC Research Notes Vol.19 No.1 (2026)
dc.identifier.doi10.1186/s13104-025-07577-6
dc.identifier.eissn17560500
dc.identifier.pmid41327378
dc.identifier.scopus2-s2.0-105026893243
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/123456789/114756
dc.rights.holderSCOPUS
dc.subjectBiochemistry, Genetics and Molecular Biology
dc.subjectMedicine
dc.titleSaSi qPCR: a novel duplex qPCR assay for rapid and simultaneous detection of Streptococcus agalactiae and Streptococcus iniae in fish
dc.typeArticle
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=105026893243&origin=inward
oaire.citation.issue1
oaire.citation.titleBMC Research Notes
oaire.citation.volume19
oairecerif.author.affiliationFaculty of Science, Mahidol University
oairecerif.author.affiliationAsian Institute of Technology Thailand
oairecerif.author.affiliationThailand National Center for Genetic Engineering and Biotechnology

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