Portable label-free electrochemical aptasensor for sensitive detection of paraquat herbicide mediated by oxygen reduction reaction
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Issued Date
2025-01-01
Resource Type
ISSN
00399140
Scopus ID
2-s2.0-85203557823
Pubmed ID
39276569
Journal Title
Talanta
Volume
281
Rights Holder(s)
SCOPUS
Bibliographic Citation
Talanta Vol.281 (2025)
Suggested Citation
Phopin K., Ruankham W., Dave J., Rukkhapiban P., Nuttavuttisit C., Hongoeb J., Zine N., Errachid A., Tantimongcolwat T. Portable label-free electrochemical aptasensor for sensitive detection of paraquat herbicide mediated by oxygen reduction reaction. Talanta Vol.281 (2025). doi:10.1016/j.talanta.2024.126841 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/102725
Title
Portable label-free electrochemical aptasensor for sensitive detection of paraquat herbicide mediated by oxygen reduction reaction
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Corresponding Author(s)
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Abstract
Paraquat (PQ) is a highly toxic herbicide that has been prohibited in almost 70 countries, but remains in use worldwide. Thus, routine on-site PQ monitoring is a key mechanism to ensure safety and efficiently enforce regulations. Herein, a label-free portable electrochemical aptasensor for the detection of PQ was developed by utilizing aptamer designed to specifically recognize PQ. The aptasensor employs square-wave voltammetry (SWV) to quantify PQ binding on the aptamer-functionalized electrode surface by tracking the downstream oxygen reduction reaction. It provided a detection range spanning from 0.01 to 100.0 μg mL−1 PQ with a limit of detection (LOD) of 8.9 ng mL−1. Validation against spiked tap water, pomegranate juice, and orange juice revealed recovery rate performances of 75 %–130 %. The aptasensor demonstrates promising feasibility for PQ detection in real-world applications, offering remarkable portability and operational simplicity. Notably, it can operate without supplementary redox agents, requiring only sample incubation and subsequent washing steps.