The PreQuine Platform: A novel diagnostic tool for measuring glucose-6-phosphate dehydrogenase (G6PD) activity and hemoglobin concentration
Issued Date
2024-01-01
Resource Type
eISSN
19326203
Scopus ID
2-s2.0-85192912615
Pubmed ID
38728310
Journal Title
PloS one
Volume
19
Issue
5
Rights Holder(s)
SCOPUS
Bibliographic Citation
PloS one Vol.19 No.5 (2024) , e0297918
Suggested Citation
Harper R., Ley B., Kabir M.A., Matulis G., von Seidlein L., Alam M.S., Adhikari B., Okech B.A., Williams A.L., Price R.N., von Fricken M.E. The PreQuine Platform: A novel diagnostic tool for measuring glucose-6-phosphate dehydrogenase (G6PD) activity and hemoglobin concentration. PloS one Vol.19 No.5 (2024) , e0297918. doi:10.1371/journal.pone.0297918 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/98426
Title
The PreQuine Platform: A novel diagnostic tool for measuring glucose-6-phosphate dehydrogenase (G6PD) activity and hemoglobin concentration
Corresponding Author(s)
Other Contributor(s)
Abstract
Quantitative diagnosis of glucose-6-phosphate dehydrogenase (G6PD) deficiency is essential for the safe administration of 8-aminoquinoline based radical cure for the treatment of Plasmodium vivax infections. Here, we present the PreQuine Platform (IVDS, USA), a quantitative biosensor that uses a dual-analyte assay for the simultaneous measurement of Hemoglobin (Hgb) levels and G6PD enzyme activity within the same sample. The platform relies on a downloadable mobile application. The device requires 10μl of whole blood and works with a reflectance-based meter. Comparing the G6PD measurement normalized by Hgb of 12 samples from the PreQuine Platform with reference measurements methods (spectrophotometry, Pointe Scientific, USA and hemoglobin meter, HemoCue, Sweden) showed a positive and significant agreement with a slope of 1.0091 and an intercept of -0.0379 under laboratory conditions. Next steps will be to conduct field trials in Bangladesh, Cambodia, and the USA to assess diagnostic performance, user friendliness and acceptance.