Evaluation of igm/igg antibodies specific lipl32 domain(s) for development of early-phase serodiagnosis of human leptospirosis
1
Issued Date
2013
Resource Type
Language
eng
Rights
Mahidol University
Suggested Citation
Nonglucksanawan Ritthisunthorn, นงลักษณวรรณ ฤทธิสุนทร, Santi Maneewatch, สันติ มณีวัชระรังสี, Poom Adisakwattana, ภูมิ อดิศักดิ์วัฒนา, Urai Chaisri, อุไร ไชยศรี, Thareerat Kalambaheti, ธารีรัตน์ กะลัมพะเหติ, Patcharin Saengjaruk, พัชรินทร์ แสงจารึก (2013). Evaluation of igm/igg antibodies specific lipl32 domain(s) for development of early-phase serodiagnosis of human leptospirosis. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/63357
Title
Evaluation of igm/igg antibodies specific lipl32 domain(s) for development of early-phase serodiagnosis of human leptospirosis
Other Contributor(s)
Mahidol University. Faculty of Tropical Medicine. Department of Molecular Tropical Medicine and Genetics
Mahidol University. Faculty of Tropical Medicine. Department of Helminthology
Mahidol University. Faculty of Tropical Medicine. Department of Tropical Pathology
Mahidol University. Faculty of Tropical Medicine. Department of Microbiology and Immunology
Mahidol University. Faculty of Tropical Medicine. Department of Helminthology
Mahidol University. Faculty of Tropical Medicine. Department of Tropical Pathology
Mahidol University. Faculty of Tropical Medicine. Department of Microbiology and Immunology
Abstract
Simple and accurate diagnosis for leptospirosis is still needed in order to differentiate the disease from
other causes of acute febrile illness. LipL32-based enzyme-linked immunosorbent assay (ELISA) provides
rapid and sensitive serologic diagnosis of human infection. It has been reported that the C-terminus
LipL32 portion is an immunodominant and elicited both LipL32-specific IgM and IgG antibodies during
earlier course of infection. In this study, LipL32-domain specific human IgM and IgG antibodies reactivity
were evaluated from MAT-positive sera by using recombinant LipL32, full-length LipL321-272 and two deletion
mutant, i.e. LipL32 Δ1-90 and LipL32Δ1-170 proteins as antigens in ELISA assay. Diagnosis efficacy of individual
antigens was evaluated. Preliminary results reveal LipL32Δ1-90 is an immunodominant recognized by IgM whereas C-terminus
LipL32Δ1-170 proteins was recognized by IgG antibodies in early phase of disease. However, more number of
samples are needed in order to definite the use of LipL32 truncation proteins in development of LipL32-
based ELISA.
Description
Joint International Tropical Medicine Meeting 2013: Towards global health: an Asian paradigm of Tropical Medicine 11-13 December 2013 Centara Grand Bangkok Convention Center at Central World, Bangkok, Thailand. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2013. p.160.