Evaluation of igm/igg antibodies specific lipl32 domain(s) for development of early-phase serodiagnosis of human leptospirosis

Abstract

Simple and accurate diagnosis for leptospirosis is still needed in order to differentiate the disease from other causes of acute febrile illness. LipL32-based enzyme-linked immunosorbent assay (ELISA) provides rapid and sensitive serologic diagnosis of human infection. It has been reported that the C-terminus LipL32 portion is an immunodominant and elicited both LipL32-specific IgM and IgG antibodies during earlier course of infection. In this study, LipL32-domain specific human IgM and IgG antibodies reactivity were evaluated from MAT-positive sera by using recombinant LipL32, full-length LipL321-272 and two deletion mutant, i.e. LipL32 Δ1-90 and LipL32Δ1-170 proteins as antigens in ELISA assay. Diagnosis efficacy of individual antigens was evaluated. Preliminary results reveal LipL32Δ1-90 is an immunodominant recognized by IgM whereas C-terminus LipL32Δ1-170 proteins was recognized by IgG antibodies in early phase of disease. However, more number of samples are needed in order to definite the use of LipL32 truncation proteins in development of LipL32- based ELISA.