Recovery and Quantification of Norovirus in Air Samples from Experimentally Produced Aerosols
Issued Date
2024-01-01
Resource Type
ISSN
18670334
eISSN
18670342
Scopus ID
2-s2.0-85188238014
Journal Title
Food and Environmental Virology
Rights Holder(s)
SCOPUS
Bibliographic Citation
Food and Environmental Virology (2024)
Suggested Citation
Rupprom K., Thongpanich Y., Sukkham W., Utrarachkij F., Kittigul L. Recovery and Quantification of Norovirus in Air Samples from Experimentally Produced Aerosols. Food and Environmental Virology (2024). doi:10.1007/s12560-024-09590-7 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/97753
Title
Recovery and Quantification of Norovirus in Air Samples from Experimentally Produced Aerosols
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Corresponding Author(s)
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Abstract
Norovirus is the leading cause of acute gastroenteritis in humans across all age groups worldwide. Norovirus-infected patients can produce aerosolized droplets which play a role in gastroenteritis transmission. The study aimed to assess bioaerosol sampling in combination with a virus concentrating procedure to facilitate molecular detection of norovirus genogroup (G) II from experimentally contaminated aerosols. Using a nebulizer within an experimental chamber, aerosols of norovirus GII were generated at known concentrations. Air samples were then collected in both 5 mL and 20 mL water using the SKC BioSampler at a flow rate of 12.5 L/min, 15 min. Subsequently, the virus in collected water was concentrated using speedVac centrifugation and quantified by RT-qPCR. The optimal distances between the nebulizer and the SKC BioSampler yielded high recoveries of the virus for both 5 and 20 mL collections. Following nebulization, norovirus GII RNA was detectable up to 120 min in 5 mL and up to 240 min in 20 mL collection. The concentrations of norovirus GII RNA recovered from air samples in the aerosol chamber ranged from 102 to 105 genome copies/mL, with average recoveries of 25 ± 12% for 5 mL and 22 ± 19% for 20 mL collections. These findings provide quantitative data on norovirus GII in aerosols and introduce a novel virus concentrating method for aerosol collection in water, thus enhancing surveillance of this virus.