DNA barcoding of the parasitic flies, stomoxys flies (diptera: muscidae), from natural sources in Thailand
Issued Date
2013
Resource Type
Language
eng
Rights
Mahidol University
Suggested Citation
Jiraporn Ruangsittichai, จิราภรณ์ เรืองสิทธิชัย, Tanasak Changbunjong, Thekhawet Weluwanarak, Chamnarn Apiwathnasorn, ชำนาญ อภิวัฒนศร, Sungsit Sungvornyothin, สังสิทธิ์ สังวรโยธิน, Patchara Sriwichai, พัชรา ศรีวิชัย, Suchada Sumruayphol, สุชาดา สำรวยผล (2013). DNA barcoding of the parasitic flies, stomoxys flies (diptera: muscidae), from natural sources in Thailand. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/63368
Title
DNA barcoding of the parasitic flies, stomoxys flies (diptera: muscidae), from natural sources in Thailand
Other Contributor(s)
Abstract
The Stomoxys flies are known as parasitic flies allecting livestock and wildlife. They have determined as
high impact in economic lost especially dairy farm. Adult flies feed on blood causing blood loss and
reducing milk product. They are major irritant pests of livestock and transmit many pathogens to
cattle. The Stomoxys flies were collected from many parts of Thailand. Species were firstly identified based on
morphological characteristics. There are some Stomoxys species, Stomoxys indicus, Stomoxys pulla and Stomoxys
uruma, which are poorly identified by morphology. DNA barcoding has been developed to solve this problem.
A 657 bp of COI barcodes from five species, Stomoxys calcitrans Stomoxys indicus, Stomoxys pulla, Stomoxys sitiens
and Stomoxys uruma, could be amplified. Their DNA sequences were made phylogenetic analysis and calculated
sequence divergence by MEGA 5.1 program. The average of interspecific sequence divergence ranged 5.2-
12.0%. The neighbor-joining (NJ) analysis among five species of the Stomoxyini flies was analysed using K2P
parameter. All of Stomoxys flies were clearly separated into each species. The DNA barcoding can be used to
confirm the poorly discriminated species and is applied for managing control programs of parasitic flies.
Description
Joint International Tropical Medicine Meeting 2013: Towards global health: an Asian paradigm of Tropical Medicine 11-13 December 2013 Centara Grand Bangkok Convention Center at Central World, Bangkok, Thailand. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2013. p.169.