Trackability of proteins from probiotic Bifidobacterium spp. in the gut using metaproteomics

Hendrickx D.M.; An R.; Boeren S.; Mutte S.K.; Wopereis H.; Belzer C.; Chatchatee P.; Nowak Wegrzyn A.; Lange L.; Benjaponpitak S.; Wee Chong K.; Sangsupawanich P.; Van Ampting M.T.J.; Oude Nijhuis M.M.; Harthoorn L.F.; Langford J.E.; Knol J.; Knipping K.; Garssen J.; Trendelenburg V.; Pesek R.; Davis C.M.; Muraro A.; Erlewyn-Lajeunesse M.; Fox A.T.; Michaelis L.J.; Beyer K.; Noimark L.; Stiefel G.; Schauer U.; Hamelmann E.; Peroni D.; Boner A.

Trackability of proteins from probiotic Bifidobacterium spp. in the gut using metaproteomics

Issued Date

2023-01-01

Resource Type

Article

ISSN

18762883

eISSN

18762891

DOI

10.1163/18762891-20220137

Scopus ID

2-s2.0-85191384129

Pubmed ID

38661361

Journal Title

Beneficial Microbes

Volume

14

Issue

3

Start Page

269

End Page

280

Rights Holder(s)

SCOPUS

Bibliographic Citation

Beneficial Microbes Vol.14 No.3 (2023) , 269-280

Suggested Citation

Hendrickx D.M., An R., Boeren S., Mutte S.K., Wopereis H., Belzer C., Chatchatee P., Nowak Wegrzyn A., Lange L., Benjaponpitak S., Wee Chong K., Sangsupawanich P., Van Ampting M.T.J., Oude Nijhuis M.M., Harthoorn L.F., Langford J.E., Knol J., Knipping K., Garssen J., Trendelenburg V., Pesek R., Davis C.M., Muraro A., Erlewyn-Lajeunesse M., Fox A.T., Michaelis L.J., Beyer K., Noimark L., Stiefel G., Schauer U., Hamelmann E., Peroni D., Boner A. Trackability of proteins from probiotic Bifidobacterium spp. in the gut using metaproteomics. Beneficial Microbes Vol.14 No.3 (2023) , 269-280. 280. doi:10.1163/18762891-20220137 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/101313

Title

Trackability of proteins from probiotic Bifidobacterium spp. in the gut using metaproteomics

Corresponding Author(s)

Hendrickx D.M.

Other Contributor(s)

Mahidol University

Abstract

Beneficial effects of Bifidobacterium spp. on gut microbiota development and infant health have been reported earlier. Therefore, supplementation of infant formulas with probiotic bifidobacteria, as well as prebiotics stimulating bifidobacterial growth, has been proposed. Here, we studied the faecal microbiome of infants supplemented with specialized nutrition, of which some received a standard amino acid-based formula (AAF) and others an AAF with a specific mixture of prebiotics and a probiotic (synbiotics) using metaproteomics and 16S rRNA gene sequencing. Faecal samples were taken at baseline, as well as after 6 and 12 months fed with the specialized formula. The aim was to compare microbial differences between infants treated with the standard AAF and those who received the additional synbiotics. Our findings show that infants who received AAF with synbiotics have significantly higher levels of Bifidobacteriaceae DNA as well as significantly increased levels of Coriobacteriaceae proteins, over time. Moreover, at visit 12 months higher levels of some bifidobacterial carbohydrate-active enzymes, known to metabolize oligosaccharides, were observed in the synbiotic group compared to the non-synbiotic group. The results indicate that the synbiotics provided in our study are trackable in faecal samples on the DNA and protein level during the intervention period.

Keyword(s)

Medicine

URI

https://repository.li.mahidol.ac.th/handle/123456789/101313

Collections

Scopus 2023

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