Trackability of proteins from probiotic Bifidobacterium spp. in the gut using metaproteomics

dc.contributor.authorHendrickx D.M.
dc.contributor.authorAn R.
dc.contributor.authorBoeren S.
dc.contributor.authorMutte S.K.
dc.contributor.authorWopereis H.
dc.contributor.authorBelzer C.
dc.contributor.authorChatchatee P.
dc.contributor.authorNowak Wegrzyn A.
dc.contributor.authorLange L.
dc.contributor.authorBenjaponpitak S.
dc.contributor.authorWee Chong K.
dc.contributor.authorSangsupawanich P.
dc.contributor.authorVan Ampting M.T.J.
dc.contributor.authorOude Nijhuis M.M.
dc.contributor.authorHarthoorn L.F.
dc.contributor.authorLangford J.E.
dc.contributor.authorKnol J.
dc.contributor.authorKnipping K.
dc.contributor.authorGarssen J.
dc.contributor.authorTrendelenburg V.
dc.contributor.authorPesek R.
dc.contributor.authorDavis C.M.
dc.contributor.authorMuraro A.
dc.contributor.authorErlewyn-Lajeunesse M.
dc.contributor.authorFox A.T.
dc.contributor.authorMichaelis L.J.
dc.contributor.authorBeyer K.
dc.contributor.authorNoimark L.
dc.contributor.authorStiefel G.
dc.contributor.authorSchauer U.
dc.contributor.authorHamelmann E.
dc.contributor.authorPeroni D.
dc.contributor.authorBoner A.
dc.contributor.correspondenceHendrickx D.M.
dc.contributor.otherMahidol University
dc.date.accessioned2024-09-22T18:10:56Z
dc.date.available2024-09-22T18:10:56Z
dc.date.issued2023-01-01
dc.description.abstractBeneficial effects of Bifidobacterium spp. on gut microbiota development and infant health have been reported earlier. Therefore, supplementation of infant formulas with probiotic bifidobacteria, as well as prebiotics stimulating bifidobacterial growth, has been proposed. Here, we studied the faecal microbiome of infants supplemented with specialized nutrition, of which some received a standard amino acid-based formula (AAF) and others an AAF with a specific mixture of prebiotics and a probiotic (synbiotics) using metaproteomics and 16S rRNA gene sequencing. Faecal samples were taken at baseline, as well as after 6 and 12 months fed with the specialized formula. The aim was to compare microbial differences between infants treated with the standard AAF and those who received the additional synbiotics. Our findings show that infants who received AAF with synbiotics have significantly higher levels of Bifidobacteriaceae DNA as well as significantly increased levels of Coriobacteriaceae proteins, over time. Moreover, at visit 12 months higher levels of some bifidobacterial carbohydrate-active enzymes, known to metabolize oligosaccharides, were observed in the synbiotic group compared to the non-synbiotic group. The results indicate that the synbiotics provided in our study are trackable in faecal samples on the DNA and protein level during the intervention period.
dc.identifier.citationBeneficial Microbes Vol.14 No.3 (2023) , 269-280
dc.identifier.doi10.1163/18762891-20220137
dc.identifier.eissn18762891
dc.identifier.issn18762883
dc.identifier.pmid38661361
dc.identifier.scopus2-s2.0-85191384129
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/123456789/101313
dc.rights.holderSCOPUS
dc.subjectMedicine
dc.titleTrackability of proteins from probiotic Bifidobacterium spp. in the gut using metaproteomics
dc.typeArticle
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85191384129&origin=inward
oaire.citation.endPage280
oaire.citation.issue3
oaire.citation.startPage269
oaire.citation.titleBeneficial Microbes
oaire.citation.volume14
oairecerif.author.affiliationGreat North Children's Hospital
oairecerif.author.affiliationNutricia Research, Netherlands
oairecerif.author.affiliationUtrechts Instituut voor Farmaceutische Wetenschappen
oairecerif.author.affiliationAzienda Ospedaliera Universitaria Integrata Verona
oairecerif.author.affiliationNYU Langone Health
oairecerif.author.affiliationSt. Marien Hospital, Bonn
oairecerif.author.affiliationAzienda Ospedale Università Padova
oairecerif.author.affiliationCharité – Universitätsmedizin Berlin
oairecerif.author.affiliationFaculty of Medicine, Prince of Songkla University
oairecerif.author.affiliationUniwersytet Warminsko-Mazurski w Olsztynie
oairecerif.author.affiliationFaculty of Medicine Ramathibodi Hospital, Mahidol University
oairecerif.author.affiliationKK Women's And Children's Hospital
oairecerif.author.affiliationThe Royal London Hospital
oairecerif.author.affiliationLeicester Royal Infirmary
oairecerif.author.affiliationGuy's and St Thomas' NHS Foundation Trust
oairecerif.author.affiliationRuhr-Universitat Bochum
oairecerif.author.affiliationWageningen University & Research
oairecerif.author.affiliationArkansas Children's Hospital
oairecerif.author.affiliationFaculty of Medicine, Chulalongkorn University
oairecerif.author.affiliationBaylor College of Medicine
oairecerif.author.affiliationUniversity Hospital Southampton NHS Foundation Trust

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