HPLC Quantification of Chemical Markers from Lysiphyllum strychnifolium
Issued Date
2022-04-01
Resource Type
eISSN
1981528X
Scopus ID
2-s2.0-85126900593
Journal Title
Revista Brasileira de Farmacognosia
Volume
32
Issue
2
Start Page
266
End Page
272
Rights Holder(s)
SCOPUS
Bibliographic Citation
Revista Brasileira de Farmacognosia Vol.32 No.2 (2022) , 266-272
Suggested Citation
Kongkiatpaiboon S., Tayana N., Inthakusol W., Duangdee N., Chewchinda S., Keeratinijakal V. HPLC Quantification of Chemical Markers from Lysiphyllum strychnifolium. Revista Brasileira de Farmacognosia Vol.32 No.2 (2022) , 266-272. 272. doi:10.1007/s43450-022-00237-x Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/86871
Title
HPLC Quantification of Chemical Markers from Lysiphyllum strychnifolium
Author's Affiliation
Other Contributor(s)
Abstract
Lysiphyllum strychnifolium (W. G. Craib) A. Schmitz (syn. Bauhinia strychnifolia), Fabaceae, vernacularly known in Thai as “Ya-Nang-Daeng,” has been traditionally used for detoxification and longevity. A high-performance liquid chromatography for the determination of four bioactive components, gallic acid, trilobatin, yanangdaengin, and astilbin, as chemical markers for leaves and stems of this medicinal plant, was developed and validated. The chromatographic separation was performed on a reversed-phase C-18 column using a gradient system of 0.5% acetic acid and methanol with a flow rate of 1 ml/min. The validated method showed good sensitivity, linearity, precision, and accuracy. The effect of solvent polarity on the yield of bioactive compounds was also studied. The gallic acid, trilobatin, and yanangdaengin contents of L. strychnifolium leaves as well as astilbin from its stems collected from different localities were analyzed. Chromatographic fingerprinting between each population indicated a homogeneity of L. strychnifolium in Thailand. [Figure not available: see fulltext.]