In vitro suppression on development of plasmodium falciparum in thalassemic erythrocytes by oxidative stress

Abstract

This study is aimed at investigating the effect of oxidative stress on the development of Plasmodium falciparum in ?-thalassemia erythrocytes in vitro. P. falciparum cultured in erythrocytes from normal (n = 16), ?-thalassemia 1 trait (n = 20), ?-thalassemia 2 trait (n = 10), Hb CS trait (n = 8) was subjected to H(,2)O(,2), generated from the reaction of glucose-glucose oxidase. Results from experiments revealed that malaria parasite in ?-thalassemia 2 trait and Hb CS trait erythrocytes were more susceptible to H(,2)O(,2) than that in ?-thalassemia 1 trait which was not significantly different from normal erythrocyte. The reduction of parasite survival was more pronounced when the thalassemia sera were used.