Biological Activities of Extracts and Secondary Metabolites from Millettia phuwuaensis

Abstract

A study of the phytochemicals constituents of the stems of, Millettia phuwuaensis has led to the isolation of 7-methoxy-5/,6/-methylenedioxyisoflavone (1), and 12-deoxo-12α-hydroxyelliptone (2. Their structures were confirmed using NMR spectroscopy. Both extracts and the purified compounds were evaluated for their antibacterial, anti-HIV, and cancer activity. The antibacterial test results of the extracts and pure compound were found to be valuable, MIC is in the range of 12.5-200 mg/mL, in the range of 0.188-6 mg/mL, respectively. Mechanistic anti-HIV affect RT and MC99 found that ethyl acetate extract inhibited the very high level with IC50 with 75.93%. It was also found that all extracts were effective in inhibiting AIDs by mechanism MC99 at EC50 at 1.35 µM (TI>2.41). Further, the ethyl acetate extract showed marked cytotoxicity (ED50 = 17.58 µg/ml against the SH-SY5Y cancer cell line. Additionally, compound 1 also exhibited RT, moderately active with IC50 55.19 % inhibition. More than that, compounds 1 and 2 also exhibited MC99 at 50% (EC50) values of > 3.01 (TI >1.70) and 1.78 (TI >1.70), respectively.