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Publication Metadata only Internalization of the dengue virus is cell cycle modulated in HepG2, but not Vero cells(2004-11-01) Waranyoo Phoolcharoen; Duncan R. Smith; Mahidol UniversityWhile many studies have investigated the relationship between cell type and dengue virus infection, no study to date has examined the effect of cell physiology on permissiveness to infection. Unsynchronized and artificially synchronized cell... populations at different stages of the cell cycle of two cell types (Vero and HepG2) were examined for permissiveness to infection by two dengue virus serotypes (serotypes 2 and 3) by determining both the levels of virus produced as well as the percentagePublication Metadata only The Aedes aegypti cell line CCL-125 is dengue virus permissive(2009-05-01) Nitwara Wikan; Atichat Kuadkitkan; Duncan R. Smith; Mahidol UniversityWhile the majority of dengue infections worldwide are transmitted by the Aedes aegypti mosquito, the majority of research into the interaction between dengue and insect cells is undertaken in the Aedes albopictus derived cell line C6/36. The CCL-125... cell line is a long established A. aegypti derived cell line that was originally characterized as not susceptible to infection by the dengue virus. The present study establishes that CCL-125 is permissive to dengue virus infection and is ablePublication Metadata only Analysis of saturation binding and saturation infection for dengue serotypes 1 and 2 in liver cells(2003-02-15) Lukkana Suksanpaisan; Duncan R. Smith; Mahidol UniversityObjective: The liver has been increasingly recognized as a significant target organ in the pathogenesis of dengue virus infection. However, only two contradictory studies have examined the binding of the dengue virus to liver cells. This study... therefore sought to investigate the binding of the dengue virus to HepG2 cells. Methods: Radio-labeled dengue virus serotypes 1 and 2 were prepared through viral propagation in Vero cells. Increasing amounts of virus were then incubated with HepG2 cellsPublication Metadata only Apoptosis in Dengue Virus Infected Liver Cell Lines HepG2 and Hep3B(2004-03-01) Thananya Thongtan; Sakol Panyim; Duncan R. Smith; Mahidol UniversityWhile both in vivo and in vitro evidence has suggested that liver cells undergo apoptosis in response to dengue virus infection, little is known about the mechanism of induction. Given that the p53 tumour suppressor gene is a key mediator... of apoptosis, we sought to define the role of this gene in response to dengue virus infection. After infection, a p53 wild type liver cell line (HepG2) showed changes consistent with apoptosis including alterations of cell morphology, cellular detachmentPublication Metadata only Investigation of the cry4B-prohibitin interaction in Aedes aegypti cells(2012-10-01) Atichat Kuadkitkan; Duncan R. Smith; Colin Berry; Mahidol University; Cardiff Universityfever, and chikungunya. Previous work has shown that Cry4B binds to several mid-gut membrane proteins in Aedes aegypti larvae including prohibitin, a protein recently identified as a receptor that also mediates entry of dengue virus into Aedes cells..., it was not cytotoxic to two Aedes cell lines, allowing determination of its effect on dengue virus infectivity in the absence of Cry4B-induced cell lysis. Preexposure of Aedes cells to Cry4B resulted in a significant reduction in the number of infected cells comparedPublication Metadata only Adaptation of the plaque assay methodology for dengue virus infected HepG2 cells(2004-03-15) Pimjai Chingsuwanrote; Lukkana Suksanpaisan; Duncan R. Smith; Mahidol UniversityThe HepG2 cell line is a useful tool for studying dengue virus-cell interactions but as it grows in clumps rather than monolayers, it does not readily adapt itself to the standard plaque assay technique. We therefore sought to develop an indirect... plaque assay methodology. Initially HepG2 cells were infected with dengue virus serotype 2 and post-infection incubated for between 0 and 16h before being treated with trypsin to separate the cells, followed by dilution and plating onto pre-grownPublication Metadata only Internalization and propagation of the dengue virus in human hepatoma (HepG2) cells(2004-07-07) Chutima Thepparit; Waranyoo Phoolcharoen; Lukkana Suksanpaisan; Duncan R. Smith; Mahidol UniversityObjectives: This study sought to undertake a comparative analysis of the internalization and propagation of all four dengue serotypes in a single cell line of human liver origin, HepG2. Methods: Virus production after infection was determined... by the plaque assay technique. Internalization profiles were determined by incubating virus and cells on ice and then raising the temperature for various times. The contribution of extracellular matrix components to internalization was determined by pretreatmentPublication Metadata only Induced autophagy reduces virus output in dengue infected monocytic cells(2011-09-15) Mingkwan Panyasrivanit; Michael P. Greenwood; David Murphy; Ciro Isidoro; Prasert Auewarakul; Duncan R. Smith; Mahidol University; University of Bristol; Universita degli Studi del Piemonte Orientale Amedeo AvogadroWhile several studies have shown a role for autophagy in the replication of dengue virus (DENV), these studies have been performed in directly infected cells. However, in severe cases of DENV infection the critical cell in the disease is believed... to be monocytes which are poorly infected directly, but are highly susceptible to antibody enhanced infection. This study sought to determine the involvement of autophagy in the DENV infection of monocytic cells, using U937 cells as a model system. WhilePublication Metadata only Induced pluripotent stem cells: A new addition to the virologists armamentarium(2016-09-01) Atichat Kuadkitkan; Nitwara Wikan; Duncan R. Smith; Mahidol University© 2016 Elsevier B.V. A significant amount of our understanding of the molecular events occurring during viral replication has originated from studies utilizing cell lines. These cell lines are normally obtained by the culture of samples from... spontaneously occurring tumors or are derived by genetic manipulation of primary cells. The genetic events inducing immortalization and/or transformation to allow continual passage in culture can have profound effects resulting in a marked loss of cell typePublication Metadata only Entry into and production of the Japanese encephalitis virus from C6/36 cells(2007-02-01) Verawan Boonsanay; Duncan R. Smith; Mahidol UniversityObjectives: The mosquito-borne Japanese encephalitis virus is a leading cause of encephalitis worldwide. However, few studies have investigated the kinetics of Japanese encephalitis virus internalization and production in mosquito cells, and fewer... still have investigated the nature of the molecules involved in the binding of the virus to mosquito cells. Methods: Using the Aedes albopictus/Stegomyia albopicta-derived C6/36 cell line, Japanese encephalitis virus internalization and production were