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Publication Metadata only Immunohistochemical detection of GnRH-like peptides in the neural ganglia and testis of Haliotis asinina(2014-02-01) Parinyaporn Nuurai; Jeerawan Primphon; Tawanchay Seangcharoen; Yotsawan Tinikul; Chaitip Wanichanon; Prasert Sobhon; Burapha University; Mahidol UniversityGonadotropin releasing hormone (GnRH) is a peptide that is conserved in both vertebrate and invertebrate species. In this study, we have demonstrated the distribution pattern of two isoforms of GnRH-like peptides in the neural ganglia and testis... of the pleuropedal ganglia. In the testis, only octGnRH-ir was found primarily in the granulated cell and central capillaries within the trabeculae. These results suggest that multiple GnRH-like peptides are present in the neural ganglia which could be the principalPublication Metadata only Rat sperm AS-A: Subcellular localization in testis and epididymis and surface distribution in epididymal sperm(2004-11-01) Apichart Ngernsoungnern; Wattana Weerachatyanukul; Arpornrad Saewu; Siriporn Thitilertdecha; Prasert Sobhon; Prapee Sretarugsa; Mahidol UniversityIn this study, we investigated the subcellular compartmentalization of arylsulfatase-A (AS-A) in the testis and epididymis as well as the surface distribution in rat epididymal sperm. Testicular AS-A was compartmentalized specifically to the areaPublication Metadata only Existence of APGWamide in the testis and its induction of spermiation in Haliotis asinina Linnaeus(2008-07-02) Piyachat Chansela; Porncharn Saitongdee; Praphaporn Stewart; Nantawan Soonklang; Michael Stewart; Worawit Suphamungmee; Tanes Poomtong; Prasert Sobhon; Mahidol University; Faculty of Medicine, Thammasat University; Deakin University; Coastal Aquaculture Research and Development CenterImmunolocalization by using rabbit polyclonal antibody against APGWamide demonstrated that there was APGWamide immunoreactivity (APGW-ir) in the varicosed nerve fibers in the capsule, connective tissue and trabeculae of the testis whereasPublication Metadata only Starvation promotes autophagy-associated maturation of the testis in the giant freshwater prawn, macrobrachium rosenbergii(2019-09-01) Wilairat Kankuan; Chaitip Wanichanon; Federica Morani; Sirorat Thongrod; Rossella Titone; Tanapan Siangcham; Matilde Masini; Michela Novelli; Prasert Sobhon; Ciro Isidoro; Università di Pisa; Mahidol University; Università degli Studi del Piemonte Orientale "Amedeo Avogadro"and cellular changes in the testis of the giant freshwater prawn Macrobrachium rosenbergii that paralleled the induction of autophagy. Under short starvation condition, the male prawns showed increased gonado-somatic index, increased size, and late stagePublication Metadata only Distribution of abalone egg-laying hormone-like peptide in the central nervous system and reproductive tract of the male mud crab, Scylla olivacea(2019-02-01) Kanjana Khornchatri; Jirawat Saetan; Tipsuda Thongbuakaew; Thanyaporn Senarai; Thanapong Kruangkum; Napamanee Kornthong; Yotsawan Tinikul; Prasert Sobhon; Walailak University; Mahidol University; Thammasat University; Burapha University; Prince of Songkla Universitypelagicus. In this study, we have studied the histology of the male reproductive tract in Scylla olivacea which are comprised of anterior testis, posterior testis, early proximal spermatic duct (ePSD), proximal spermatic duct (PSD), middle spermatic duct... (MSD) and distal spermatic duct (DSD), by immunohistochemistry, detected an abalone ELH- immunoreactivity (aELH-ir) in epithelium of ducts in posterior testis and epithelium of all parts of spermatic duct. Furthermore, we could detect aELH-ir in neuronsPublication Metadata only Presence of gonadotropin-releasing hormone-like peptide in the central nervous system and reproductive organs of the male blue swimming crab, Portunus pelagicus, and its effect on spermatogenesis(2016-08-01) Thanyaporn Senarai; Jirawat Saetan; Montakan Tamtin; Wattana Weerachatyanukul; Prasert Sobhon; Prepee Sretarugsa; Mahidol University; Prince of Songkla University; Coastal Fisheries Research and Development Bureau; Burapha Universityin neurons of the dorso-lateral and ventro-medial clusters. In the thoracic ganglia, lGnRH-III-ir was observed in the large-sized neurons between the thoracic neuropils and in the ventromedial cluster of the abdominal ganglia. In the testis, lGnRH-III-ir... was detected in nurse cells, hemocytes, spermatids 2, and the outer and inner zones of the acrosomes of spermatozoa. Bioassay showed that lGnRH-III significantly increased the testis-somatic index, the percentage of late stages of seminiferous tubules (stagesPublication Open Access Morphology and histology of the adult Paramphistomum gracile Fischoeder, 1901.(2013) Busaba Panyarachun; Arin Ngamniyom; Prasert Sobhon; Panat Anuracpreeda; Mahidol University. Kanchanaburi Campus. Agricultural Science; Mahidol University.Faculty of Science. Department of Anatomy; Mahidol University.Division of Agricultural Science.In the present study, we evaluated the histological morphology of the adult Paramphistomum (P.) gracile. Adult flukes with bodies 5∼15 mm in length and 2∼7 mm in width were subjected to histological analysis. Longitudinal and transversal serial-sections were stained with hematoxylin and eosin, and examined. The body surface and longitudinal section of P. gracile were also assessed using scanning electron microscopy. In this species, the anterior sucker and posterior sucker (acetabulum) were present on an anterior and posterior part of the body, respectively. The major folds were located in the areas of the anterior sucker, genital canal, and posterior sucker. The fluke membrane was spineless at the tegument surface and in the tegument tissue. Histological data showed structural-systematic characteristics of the digestive tract, reproductive tract, excretory tract, copulatory organs, connective tissues, and muscle tissues. We attempted to elucidate the histological characteristics of P. gracile that might increase the knowledge and understanding of rumen fluke morphology.Publication Metadata only Changes of phosphatidylcholine and fatty acids in germ cells during testicular maturation in three developmental male morphotypes of Macrobrachium rosenbergii revealed by imaging mass spectrometry(2015-03-17) Tanapan Siangcham; Piyachat Chansela; Takahiro Hayasaka; Noritaka Masaki; Morakot Sroyraya; Jaruwan Poljaroen; Saowaros Suwansa-ard; Attakorn Engsusophon; Peter J. Hanna; Prasert Sobhon; Mitsutoshi Setou; Mahidol University; Phramongkutklao College of Medicine; Hokkaido University; Hamamatsu University School of Medicine; Deakin University© 2015 Siangcham et al. Testis maturation, germ cell development and function of sperm, are related to lipid composition. Phosphatidylcholines (PCs) play a key role in the structure and function of testes. As well, increases of polyunsaturated fatty... will be useful in formulating diets containing PUFA and HUFA for prawn broodstocks in order to improve testis development, and lead to increased male fecundity.Publication Metadata only Characterization of trabecular cells in the gonad of Haliotis Asinina linnaeus(2001-12-01) Somjai Apisawetakan; Malee Chanpoo; Chaiti Wanichanon; Vichai Linthong; Maleeya Kruatrachue; Edward Suchart Upatham; Tenate Pumthong; Prasert Sobhon; Mahidol UniversityTrabeculae are the connective tissue sheets that extend perpendicularly from the outer capsules of both the testis and ovary to make contact at their innermost ends with the inner capsules separating the gonad from the hepatopancreas. ThusPublication Metadata only Analysis of the expression and antioxidant activity of 2-Cys peroxiredoxin protein in Fasciola gigantica(2014-01-01) Kant Sangpairoj; Narin Changklungmoa; Rapeepun Vanichviriyakit; Prasert Sobhon; Kulathida Chaithirayanon; Mahidol University, moderately expressed in cecal epithelial cells in early, juvenile and adult worms. Furthermore, FgPrxs was also detected in the female reproductive organs, including eggs, ovary, vitelline cells, and testis, suggesting that FgPrxs might play an essential role
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