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Publication Metadata only Production and characterization of anti-dengue capsid antibodies suggesting the N terminus region covering the first 20 amino acids of dengue virus capsid protein is predominantly immunogenic in mice(2009-06-30) Chunya Puttikhunt; Prapapun Ong-ajchaowlerd; Tanapan Prommool; Sutha Sangiambut; Janjuree Netsawang; Thawornchai Limjindaporn; Prida Malasit; Watchara Kasinrerk; Faculty of Medicine, Siriraj Hospital, Mahidol University; Mahidol University; Chiang Mai University; Armed Forces Research Institute of Medical Sciences, ThailandWe produced monoclonal and polyclonal antibodies to the capsid (C) protein of dengue serotype 2 virus (DV2 C). First, a maltose-binding protein fused to DV2 C protein (MBP-C) was overproduced in E. coli. The affinity-purified MBP-C protein... was cleaved by factor Xa protease to obtain a recombinant DV2 C protein, which was then used for mouse immunizations. Two hybridoma cell lines producing anti-C Mabs as well as anti-C polyclonal antibody were successfully generated and characterizedPublication Metadata only Sustained replication of dengue pseudoinfectious virus lacking the capsid gene by trans-complementation in capsid-producing mosquito cells(2013-06-01) Sutha Sangiambut; Amporn Suphatrakul; Rungtawan Sriburi; Poonsook Keelapang; Chunya Puttikhunt; Watchara Kasinrerk; Prida Malasit; Nopporn Sittisombut; Thailand National Center for Genetic Engineering and Biotechnology; Chiang Mai University; Mahidol Universityof the dengue virus capsid protein served as host cells for the trans-complementation of partially capsid-deleted viral RNA generated in vitro. Transfection of the partially capsid-deleted viral RNA into the anchored capsid-expressing C6/36 cells resulted... in moderate titers of infectious virus. Progeny viruses multiplied in the capsid trans-complementing C6/36 cells for up to three weeks, but only initiated single rounds of replication in Vero cells lacking the capsid protein. Employing this transPublication Metadata only Multiple regions in dengue virus capsid protein contribute to nuclear localization during virus infection(2008-05-01) Sutha Sangiambut; Poonsook Keelapang; John Aaskov; Chunya Puttikhunt; Watchara Kasinrerk; Prida Malasit; Nopporn Sittisombut; Thailand National Center for Genetic Engineering and Biotechnology; Chiang Mai University; Queensland University of Technology QUT; Mahidol UniversityDuring infection, the capsid (C) protein of many flaviviruses localizes to the nuclei and nucleoli of several infected cell lines; the underlying basis and significance of C protein nuclear localization remain poorly understood. In this study..., double alanine-substitution mutations were introduced into three previously proposed nuclear-localization signals (at positions 6-9, 73-76 and 85-100) of dengue virus C protein, and four viable mutants, c(K6A,K7A), c(K73A,K74A), c(R85A,K86A) and c(R97A,RPublication Metadata only Nuclear localization of dengue virus capsid protein is required for DAXX interaction and apoptosis(2010-02-01) Janjuree Netsawang; Sansanee Noisakran; Chunya Puttikhunt; Watchara Kasinrerk; Wiyada Wongwiwat; Prida Malasit; Pa thai Yenchitsomanus; Thawornchai Limjindaporn; Faculty of Medicine, Siriraj Hospital, Mahidol University; Mahidol University; Thailand National Center for Genetic Engineering and Biotechnology; Chiang Mai UniversityDengue virus capsid protein (DENVC) localizes to both the cytoplasm and nucleus of dengue virus-infected cells. DENV C contains three nuclear localization signals (NLS),6KKAR9,73KKSK76, and the bipartite signal85RKeigrmlnilnRRRR100. Stable HepG2... cells, the mutant DENV Cs were restricted to the cytoplasm. The loss of nuclear localization of both mutant DENV Cs resulted in the disruption of their interactions with the apoptotic protein Daxx. Interestingly, upon treatment with anti-Fas antibodyPublication Metadata only Increased capsid oligomerization is deleterious to dengue virus particle production(2021-01-01) Sutha Sangiambut; Natcha Promphet; Suwipa Chaiyaloom; Chunya Puttikhunt; Panisadee Avirutnan; Watchara Kasinrerk; Nopporn Sittisombut; Prida Malasit; Siriraj Hospital; Thailand National Center for Genetic Engineering and Biotechnology; Thailand National Science and Technology Development Agency; Chiang Mai UniversityThe capsid protein (C) of dengue virus is required for viral infectivity as it packages viral RNA genome into infectious particles. C exists as a homodimer that forms via hydrophobic interactions between the α2 and α4 helices of monomersPublication Metadata only Comparison of penaeid shrimp and insect parvoviruses suggests that viral transfers may occur between two distantly related arthropod groups(2002-08-03) Songsak Roekring; Linda Nielsen; Leigh Owens; Sa Nga Pattanakitsakul; Prida Malasit; T. W. Flegel; Mahidol University; James Cook University, Australia; Faculty of Medicine, Siriraj Hospital, Mahidol Universityconstructed by comparing DNA genome sequences for putative viral polypeptides, capsid proteins and nonstructural proteins placed the parvoviruses into two Clades: Clade 1 with SMVmon, PfDNV, DsDNV, GmDNV, JcDNV, and BmDNV; and Clade 2 with HPVmon, HPVchinPublication Metadata only Cell death gene expression profile: Role of RIPK2 in dengue virus-mediated apoptosis(2011-03-01) Atthapan Morchang; Umpa Yasamut; Janjuree Netsawang; Sansanee Noisakran; Wiyada Wongwiwat; Pucharee Songprakhon; Chatchawan Srisawat; Chunya Puttikhunt; Watchara Kasinrerk; Prida Malasit; Pa Thai Yenchitsomanus; Thawornchai Limjindaporn; Mahidol University; Thailand National Center for Genetic Engineering and Biotechnology; Chiang Mai University; Rungsit University. The inhibition of RIPK2 expression by SB203580 significantly reduced apoptosis. SB203580 also significantly reduced DENV capsid protein (DENVC)-mediated apoptosis. Suppression of endogenous RIPK2 in DENV-infected HepG2 cells by small interfering RNA (si..., including RIPK2, HRK, TGF-β, PERK, and LC3B, was confirmed by quantitative real-time PCR. RIPK2 belongs to the receptor-interacting protein family of serine/threonine protein kinases, which is a crucial mediator of multiple stress responses that leadsPublication Metadata only Enhanced production of infectious particles by adaptive modulation of C-prM processing and C-C interaction during propagation of dengue pseudoinfectious virus in stable CprME-expressing cells(2020-01-01) Sutha Sangiambut; Chatpong Pethrak; Chainarong Anupap; Parichat Ninnabkaew; Charuphan Kongsanthia; Natcha Promphet; Suwipa Chaiyaloom; Adisak Songjaeng; Panisadee Avirutnan; Chunya Puttikhunt; Watchara Kasinrerk; Nopporn Sittisombut; Prida Malasit; Thailand National Center for Genetic Engineering and Biotechnology; Faculty of Medicine, Siriraj Hospital, Mahidol University; Chiang Mai UniversityDengue virus assembly involves the encapsidation of genomic RNA by the capsid protein (C) and the acquisition of an envelope comprising the premembrane (prM) and envelope (E) glycoproteins. This rapid process, lacking in detectable nucleocapsid... intermediates, may impose authentic C-prM-E arrangement as a prerequisite for efficient particle assembly. A mosquito cell-based complementation system was employed in this study to investigate the possibility that expression of the three structural proteinsPublication Metadata only Role of CD137 signaling in dengue virus-mediated apoptosis(2011-07-08) Amar Nagila; Janjuree Netsawang; Chatchawan Srisawat; Sansanee Noisakran; Atthapan Morchang; Umpa Yasamut; Chunya Puttikhunt; Watchara Kasinrerk; Prida Malasit; Pa thai Yenchitsomanus; Thawornchai Limjindaporn; Mahidol University; Rangsit University; Thailand National Center for Genetic Engineering and Biotechnology; Chiang Mai University-mediated tissue injury. DENV capsid protein (DENV C) physically interacts with the human death domain-associated protein Daxx. A double substitution mutation in DENV C (R85A/K86A) abrogates Daxx interaction, nuclear localization and apoptosis. Therefore we