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Publication Metadata only Activation of cryptic phthoxazolin A production in Streptomyces avermitilis by the disruption of autoregulator-receptor homologue AvaR3(2017-12-01) Dian Anggraini Suroto; Shigeru Kitani; Kiyoko T. Miyamoto; Yasuko Sakihama; Masayoshi Arai; Haruo Ikeda; Takuya Nihira; Osaka University; Hokkaido University; Kitasato University; Mahidol Universitypolyketide antibiotics (avermectin, filipin, and oligomycin) but has the potential to produce more secondary metabolites based on the number of cryptic biosynthetic gene clusters. Here, we extensively investigated the metabolite profiles of a gene disruptantPublication Metadata only Control of secondary metabolism by farX, which is involved in the γ-butyrolactone biosynthesis of Streptomyces lavendulae FRI-5(2010-03-01) Shigeru Kitani; Masashi Doi; Tomohito Shimizu; Asa Maeda; Takuya Nihira; Osaka University; Mahidol Universityare proposed to encode enzymes involved in γ-butyrolactone biosynthesis. Disruption of farX caused overproduction of D-cycloserine, and abolished production of nucleoside antibiotic and blue pigment with the loss of IM-2 production. The Wnding that all... phenotypic changes observed in the farX disruptant were restored by the addition of exogenous IM-2 suggested that FarX plays a biosynthetic role in IM-2 production. Transcriptional comparison between the wild-type strain and the farX disruptant revealedPublication Metadata only The autoregulator receptor homologue AvaR3 plays a regulatory role in antibiotic production, mycelial aggregation and colony development of Streptomyces avermitilis(2011-08-01) Kiyoko T. Miyamoto; Shigeru Kitani; Mamoru Komatsu; Haruo Ikeda; Takuya Nihira; Osaka University; Kitasato University; Mahidol Universitythe characterization of avaR3, one of the clustered receptor genes, which encodes a protein containing an extra stretch of amino acid residues that has not been found in the family of autoregulator receptors. Disruption of avaR3 resulted in markedly decreased... production of avermectins, with delayed expression of avermectin biosynthetic genes, suggesting that AvaR3 positively controls the avermectin biosynthetic genes. Moreover, the disruption caused increased production of filipin without any changesPublication Metadata only Hierarchical control of virginiamycin production in Streptomyces virginiae by three pathway-specific regulators: VmsS, VmsT and VmsR(2009-08-10) Nattika Pulsawat; Shigeru Kitani; Eriko Fukushima; Takuya Nihira; Osaka University; Mahidol Universityof the antibiotic virginiamycin, which is composed of virginiamycin M (VM) and virginiamycin S (VS), in Streptomyces virginiae. Disruption of vmsS abolished both VM and VS biosynthesis, with drastic alteration of the transcriptional profile for virginiamycin... biosynthetic genes, whereas disruption of vmsT resulted in only a loss of VM biosynthesis, suggesting that vmsS is a pathway-specific regulator for both VM and VS biosynthesis, and that vmsT is a pathway-specific regulator for VM biosynthesis alone. GenePublication Metadata only Characterization of the biosynthetic gene cluster for cryptic phthoxazolin a in streptomyces avermitilis(2018-01-01) Dian Anggraini Suroto; Shigeru Kitani; Masayoshi Arai; Haruo Ikeda; Takuya Nihira; Osaka University; Mahidol University; Kitasato University) biosynthetic gene cluster through genome sequencing, comparative genomic analysis, and gene disruption. Sequence analysis uncovered that the putative ptx biosynthetic genes are laid on an extra genomic region that is not found in the public database, and 8 open... reading frames in the extra genomic region could be assigned roles in the biosynthesis of the oxazole ring, triene polyketide and carbamoyl moieties. Disruption of the ptxA gene encoding a discrete acyltransferase resulted in a complete lossPublication Metadata only Identification of the bkdAB gene cluster, a plausible source of the starter-unit for virginiamycin M production in Streptomyces virginiae(2007-06-01) Nattika Pulsawat; Shigeru Kitani; Hiroshi Kinoshita; Chang Kwon Lee; Takuya Nihira; Osaka University; College of Medicine and Institute of Biomedical Science and Technology; Mahidol Universitydisruption of bkdA completely abolished the production of virginiamycin M (a polyketide-peptide antibiotic), while the production of virginiamycin S (a cyclodepsipeptide antibiotic) was unaffected. Complementation of the bkdA disruptant by genome-integrationPublication Metadata only Identification by gene deletion analysis of barS2, a gene involved in the biosynthesis of γ-butyrolactone autoregulator in Streptomyces virginiae(2008-04-01) Yong Jik Lee; Shigeru Kitani; Hiroshi Kinoshita; Takuya Nihira; Osaka University; Mahidol Universityisland which controls the virginiamycin biosynthesis/resistance of S. virginiae, and analyzed by gene disruption/complementation. The barS2 gene is flanked by barS1, another VB biosynthetic gene catalyzing stereospecific reduction of an A-factor-type... gene arrangement to that in the regulatory island of S. virginiae. A barS2-disruptant (strain IC152), created by means of homologous recombination, showed no differences in growth in liquid medium or morphology on solid medium compared to a wild-typePublication Metadata only Characterization of the biosynthetic gene cluster for maklamicin, a spirotetronate-class antibiotic of the endophytic Micromonospora sp. NBRC 110955(2015-01-01) Ratama Daduang; Shigeru Kitani; Junko Hashimoto; Arinthip Thamchaipenet; Yasuhiro Igarashi; Kazuo Shin-ya; Haruo Ikeda; Takuya Nihira; Osaka University; Japan Biological Informatics Consortium (JBIC); Kasetsart University; Toyama Prefectural University; Kitasato University; Mahidol Universitydifferent from other spirotetronates. Here we describe identification and characterization of the maklamicin biosynthetic (mak) gene cluster through draft genome sequencing, genomic library screening, and gene disruption. Sequence analysis revealed that a... of maklamicin production. Disruption of the polyketide synthase (PKS) genes makA1 or makA4 resulted in a complete loss of maklamicin production, indicating that the type I modular PKS system is responsible for the biosynthesis of maklamicin. The mak gene clusterPublication Metadata only VisG is essential for biosynthesis of virginiamycin S, a streptogramin type B antibiotic, as a provider of the nonproteinogenic amino acid phenylglycine(2011-11-01) Fitria Ningsih; Shigeru Kitani; Eriko Fukushima; Takuya Nihira; Osaka University; Mahidol Universitythat governs virginiamycin production. Gene deletion of visG resulted in complete loss of VS production without any changes in VM production, suggesting that visG is required for VS biosynthesis. The abolished VS production in the visG disruptant was fullyPublication Metadata only Butenolides from Streptomyces albus J1074 act as external signals to stimulate avermectin production in Streptomyces avermitilis(2018-05-01) Thao Bich Nguyen; Shigeru Kitani; Shuichi Shimma; Takuya Nihira; Osaka University; Mahidol Universityproduce butenolide-type Streptomyces hormones. Here, we describe metabolite profiling of a disruptant of the S. albus aco gene, which encodes a key biosynthetic enzyme for butenolide-type Streptomyces hormones, and identify four butenolide compounds from