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Publication Metadata only Short report: Comparison of performance of serum and plasma in panbio dengue and Japanese encephalitis virus enzyme-linked immunosorbent assays(2012-09-01) Stuart D. Blacksell; Sue J. Lee; Anisone Chanthongthip; Thaksinaporn Taojaikong; Soulignasack Thongpaseuth; Tanja Hübscher; Paul N. Newton; Mahidol University; University of Oxford; Mahosot Hospital; Hohmad ClinicWe examined the comparative performance of serum and plasma (in dipotassium EDTA) in Panbio Dengue enzyme-linked immunosorbent assays (ELISAs) for detection of non-structural protein 1 (NS1), IgM, and IgG, and a dengue/Japanese encephalitis virusPublication Metadata only Evaluation of the Panbio dengue virus nonstructural 1 antigen detection and immunoglobulin M antibody enzyme-linked immunosorbent assays for the diagnosis of acute dengue infections in Laos(2008-01-01) Stuart D. Blacksell; Mammen P. Mammen; Soulignasack Thongpaseuth; Robert V. Gibbons; Richard G. Jarman; Kemajittra Jenjaroen; Ananda Nisalak; Rattanaphone Phetsouvanh; Paul N. Newton; Nicholas P.J. Day; Mahosot Hospital; Churchill Hospital; Mahidol University; Armed Forces Research Institute of Medical Sciences, ThailandWe evaluated 2 commercial enzyme-linked immunosorbent assays (ELISAs) for the diagnosis of dengue infection; one a serologic test for immunoglobulin M (IgM) antibodies, the other based on detection of dengue virus nonstructural 1 (NS1) antigenPublication Metadata only Comparison of seven commercial antigen and antibody enzyme-linked immunosorbent assays for detection of acute dengue infection(2012-05-01) Stuart D. Blacksell; Richard G. Jarman; Robert V. Gibbons; Ampai Tanganuchitcharnchai; Mammen P. Mammen; Ananda Nisalak; Siripen Kalayanarooj; Mark S. Bailey; Ranjan Premaratna; H. Janaka De Silva; Nicholas P J Day; David G. Lalloog; Mahidol University; University of Oxford; Armed Forces Research Institute of Medical Sciences, Thailand; Queen Sirikit National Institute of Child Health; Royal Centre for Defence Medicine; University of Kelaniya; Liverpool School of Tropical MedicineSeven commercial assays were evaluated to determine their suitability for the diagnosis of acute dengue infection: (i) the Panbio dengue virus Pan-E NS1 early enzyme-linked immunosorbent assay (ELISA), second generation (Alere, Australia); (iiPublication Metadata only Serologic study of pig-associated viral zoonoses in Laos(2012-06-01) James V. Conlan; Khamphouth Vongxay; Richard G. Jarman; Robert V. Gibbons; Ross A. Lunt; Stanley Fenwick; R. C.Andrew Thompson; Stuart D. Blacksell; Murdoch University; Ministry of Agriculture and Forestry; Armed Forces Research Institute of Medical Sciences, Thailand; CSIRO Australian Animal Health Laboratory; Mahidol Universityseroprevalence was 21.1% (95% CI = 18.1-24.0%). Antibodies to SIV were detected in 1.8% (95% CI = 0.8-2.8%) of pigs by screening enzyme-linked immunosorbent assay, and only subtype H3N2 was detected by hemagglutination inhibition in two animals... with an inconclusive enzyme-linked immunosorbent assay result. No NiV antibody-positive pigs were detected. Our evidence indicates that peak JEV and HEV transmission coincides with the start of the monsoonal wet season and poses the greatest risk for human infectionPublication Metadata only Diagnostic Accuracy of the InBios Scrub Typhus Detect Enzyme-Linked Immunoassay for the Detection of IgM Antibodies in Northern Thailand(2016-02-01) Stuart D. Blacksell; Ampai Tanganuchitcharnchai; Pruksa Nawtaisong; Pacharee Kantipong; Achara Laongnualpanich; Nicholas P.J. Day; Daniel H. Paris; Mahidol University; Nuffield Department of Clinical Medicine; Chiangrai Prachanukhru Hospital; University of Notre DameCopyright © 2016 American Society for Microbiology. All Rights Reserved. In this study, we examined the diagnostic accuracy of the InBios Scrub Typhus Detect IgM enzyme-linked immunosorbent assay (ELISA) and determined the optimal diagnostic optical... immunofluorescence assay (IFA), revealed strong statistical correlation of ELISA OD values and IFA IgM titers. Determination of the optimal ELISA cutoff for seroepidemiology or screening purposes compared to the corresponding IFA reciprocal titer of 400 as previouslyPublication Metadata only Poor diagnostic accuracy of commercial antibody-based assays for the diagnosis of acute Chikungunya infection(2011-10-01) Stuart D. Blacksell; Ampai Tanganuchitcharnchai; Richard G. Jarman; Robert V. Gibbons; Daniel H. Paris; Mark S. Bailey; Nicholas P J Day; Ranjan Premaratna; David G. Lalloo; H. Janaka De Silva; Mahidol University; University of Oxford; Armed Forces Research Institute of Medical Sciences, Thailand; Royal Centre for Defence Medicine; University of Kelaniya; Liverpool School of Tropical Medicine.0%. The enzyme-linked immunosorbent assay (ELISA) gave an acute sample SN of 3.9% and an SP of 92.5% and a convalescent sample SN of 84% and an SP of 91%. These assays are not suitable for the acute diagnosis of Chikungunya virus infection. Copyright © 2011Publication Metadata only Limited diagnostic capacities of two commercial assays for the detection of Leptospira immunoglobulin M antibodies in Laos(2006-10-01) Stuart D. Blacksell; Lee Smythe; Rattanaphone Phetsouvanh; Michael Dohnt; Rudy Hartskeerl; Meegan Symonds; Andrew Slack; Manivanh Vongsouvath; Viengmone Davong; Olay Lattana; Simmaly Phongmany; Valy Keolouangkot; Nicholas J. White; Nicholas P.J. Day; Paul N. Newton; Mahosot Hospital; University of Oxford; Mahidol University; World Health Organization, Australia; Royal Tropical Institute - KITThe diagnostic utility of immunochromatographic (Leptotek) and enzyme-linked immunosorbent assay (ELISA; Panbio) tests for the detection of Leptospira immunoglobulin M antibodies was assessed in febrile adults admitted in Vientiane, Laos. Both testsPublication Metadata only The comparative accuracy of 8 commercial rapid immunochromatographic assays for the diagnosis of acute dengue virus infection(2006-04-15) Stuart D. Blacksell; Paul N. Newton; David Bell; James Kelley; Mammen P. Mammen; David W. Vaughn; Vanaporn Wuthiekanun; Amornwadee Sungkakum; Ananda Nisalak; Nicholas P.J. Day; Nuffield Department of Clinical Medicine; Mahidol University; Armed Forces Research Institute of Medical Sciences, Thailand; Mahosot Hospital; Organisation Mondiale de la Sante; U.S. Army Medical Research and Materiel Commandand nondengue serum specimens, and results were compared with those of a previously published dengue IgM/IgG enzyme-linked immunosorbent assay in conjunction with dengue virus serotyping by reverse-transcriptase polymerase chain reaction. Results. AssayPublication Metadata only A prospective assessment of the accuracy of commercial IgM ELISAs in diagnosis of Japanese encephalitis virus infections in patients with suspected central nervous system infections in Laos(2012-07-01) Catrin E. Moore; Stuart D. Blacksell; Thaksinaporn Taojaikong; Richard G. Jarman; Robert V. Gibbons; Sue J. Lee; Vilada Chansamouth; Soulignasack Thongpaseuth; Mayfong Mayxay; Paul N. Newton; Mahosot Hospital; Mahidol University; Armed Forces Research Institute of Medical Sciences, ThailandJapanese encephalitis virus (JEV) is a major cause of encephalitis in Asia. We estimated the diagnostic accuracy of two anti-JEV immunoglobulin M (IgM) enzyme-linked immunosorbent assays (ELISAs) (Panbio and XCyton JEVCheX) compared with a referencePublication Metadata only The validity of diagnostic cut-offs for commercial and in-house scrub typhus IgM and IgG ELISAs: A review of the evidence(2019-02-01) Kartika Saraswati; Meghna Phanichkrivalkosil; Nicholas P.J. Day; Stuart D. Blacksell; Eijkman Institute for Molecular Biology; Mahidol University; Nuffield Department of Clinical Medicine. The enzyme-linked immunosorbent assay (ELISA) is an objective and reproducible means of detecting IgM or IgG antibodies. However, lack of standardization in ELISA methodology, as well as in the choice of reference test with which the ELISA is compared, calls