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Publication Metadata only Rat sperm AS-A: Subcellular localization in testis and epididymis and surface distribution in epididymal sperm(2004-11-01) Apichart Ngernsoungnern; Wattana Weerachatyanukul; Arpornrad Saewu; Siriporn Thitilertdecha; Prasert Sobhon; Prapee Sretarugsa; Mahidol UniversityIn this study, we investigated the subcellular compartmentalization of arylsulfatase-A (AS-A) in the testis and epididymis as well as the surface distribution in rat epididymal sperm. Testicular AS-A was compartmentalized specifically to the areaPublication Metadata only Cathepsin D in human reproductive tissues: Cellular localization in testis and epididymis and surface distribution in different sperm conditions(2012-07-01) Arpornrad Saewu; Somluk Asuvapongpatana; Charoonroj Chotwiwatthanakun; Anuphana Tantiwongse; Wattana Weerachatyanukul; Siriporn Thitilertdecha; Faculty of Medicine, Siriraj Hospital, Mahidol University; Mahidol UniversityMammalian sperm surface antigens are acquired either during spermatogenesis or sperm maturation in the epididymis. These antigens, many of which are hydrolytic enzymes, are actively synthesized and secreted by the resident epithelial cells and adsorbed to the sperm membrane as part of posttesticular sperm modification. In this study, we aimed to investigate the expression of cathepsin-D (CAT-D) in human reproductive tissues and its distribution on the sperm surface in different sperm conditions. Immunohistochemical results revealed the expression of CAT-D in the somatic Sertoli and Leydig cells without showing any immunoreactivity in any germ cells, despite their engagement of the acrosomal system. A strong immunoreactivity of anti-CAT-D was also detected in the epididymal epithelium, chiefly in the principal cells, which are known to actively synthesize and secrete proteins into the epididymal lumen. The absence of CAT-D in the clear cells was unexpected because these cells are known to engage the endosomal machinery. We further showed that CAT-D was anchored on the sperm surface confined to the postacrosomal region without any lateral redistribution within the membrane during sperm capacitation. However, the enzyme underwent changes to be an active form of a 29/30-kd doublet during sperm capacitation. Using CAT-D as a marker, we were able to demonstrate here localization of the enzyme in human reproductive tissues, as well as reveal membrane modification in human sperm during maturation and capacitation processes. © American Society of Andrology.Publication Metadata only Presence of gonadotropin-releasing hormone-like peptide in the central nervous system and reproductive organs of the male blue swimming crab, Portunus pelagicus, and its effect on spermatogenesis(2016-08-01) Thanyaporn Senarai; Jirawat Saetan; Montakan Tamtin; Wattana Weerachatyanukul; Prasert Sobhon; Prepee Sretarugsa; Mahidol University; Prince of Songkla University; Coastal Fisheries Research and Development Bureau; Burapha Universityin neurons of the dorso-lateral and ventro-medial clusters. In the thoracic ganglia, lGnRH-III-ir was observed in the large-sized neurons between the thoracic neuropils and in the ventromedial cluster of the abdominal ganglia. In the testis, lGnRH-III-ir... was detected in nurse cells, hemocytes, spermatids 2, and the outer and inner zones of the acrosomes of spermatozoa. Bioassay showed that lGnRH-III significantly increased the testis-somatic index, the percentage of late stages of seminiferous tubules (stagesPublication Metadata only Spermatogenesis and distinctive mature sperm in the giant freshwater prawn, Macrobrachium rosenbergii (De Man, 1879)(2010-08-01) Jaruwan Poljaroen; Rapeepun Vanichviriyakit; Yotsawan Tinikul; Ittipon Phoungpetchara; Vichai Linthong; Wattana Weerachatyanukul; Prasert Sobhon; Mahidol UniversityThe structures of differentiating male germ cells in the testis of the giant freshwater prawn, Macrobrachium rosenbergii, were studied by light and electron microscopy. Based on ultrastructural characteristics, the developing male germ cellsPublication Metadata only Expression of Penaeus monodon ortholog of Niemann-Pick type C-2 in the spermatic tract, and its role in sperm cholesterol removal(2016-03-01) Charoonroj Chotwiwatthanakun; Jutharat Sangatit; Wanida Santimanawong; Piyaporn Surinlert; Juthatip Prommoon; Wattana Weerachatyanukul; Boonsirm Withyachumnarnkul; Rapeepun Vanichviriyakit; Mahidol University; Shrimp Genetic Improvement Center; Aquatic Animal Biotechnology Research Centeris expressed in many tissues, including the spermatic tract (i.e., testis, vas deferens, terminal ampoule) and the female thelycum. In situ hybridization revealed the presence of Pmnpc2 transcripts in the vas deferens, terminal ampoule, and thelycum epitheliaPublication Metadata only Cathepsin D in prawn reproductive system: its localization and function in actin degradation(2020-11-11) Chompoonut Sukonset; Piyaporn Surinlert; Orawan Thongsum; Atthaboon Watthammawut; Monsicha Somrit; Jirasuda Nakeim; Wattana Weerachatyanukul; Somluk Asuvapongpatana; Mahidol University; Thammasat University; Srinakharinwirot University; Buraphar Universityat the junction between germ cells and supporting cells and spermatogonia in M. rosenbergii testicular epithelium. Together, we have localized MrCAT-D transcript and its translational product in both supporting and germ cells of testis and claimed its enzymaticPublication Metadata only Acquisition of arylsulfatase A onto the mouse sperm surface during epididymal transit(2003-10-01) Wattana Weerachatyanukul; Hongbin Xu; Araya Anupriwan; Euridice Carmona; Michael Wade; Louis Hermo; Solange Maria Da Silva; Peter Rippstein; Prasert Sobhon; Prapee Sretarugsa; Nongnuj Tanphaichitr; Ottawa Hospital Research Institute; University of Ottawa, Canada; Health Canada; McGill University; Mahidol University; The Ottawa HospitalArylsulfatase A (AS-A) is localized to the sperm surface and participates in sperm-zona pellucida binding. We investigated how AS-A, usually known as an acrosomal enzyme, trafficked to the sperm surface. Immunocytochemistry of the mouse testisPublication Metadata only Changes of fatty acids in phosphatidylcholine on sperm membrane during Macrobrachium rosenbergii sperm transit through spermatic duct and lipid analysis in spermatic vesicles(2016-04-01) Piyaporn Surinlert; Somluk Asuvapongpatana; Nopparat Srakaew; Thanya Daungchinda; Mitsutoshi Setou; Wattana Weerachatyanukul; Mahidol University; Kasetsart University; Thaksin University; Hamamatsu University School of Medicinephophatidylcholine (PC) in Macrobrachium rosenbergii sperm collected from testis (Tsp) and vas deferens (Vsp) to give an insight of membrane lipid dynamic during sperm maturation. Saturated (SAT, predominantly C16:0) and monounsaturated fatty acids (MUFA