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Item Metadata only Human sperm fertility testing : comparison among conventional semen analysis, zona-free hamster egg penetration assay and a simple in vitro assay mehtod(Mahidol University. Mahidol University Library and Knowledge Center, 2023) Charoensri Thonabulsombat; Kanok Pavasuthipaisit; Krisada Ratana-olarnTo search for a simple, convenient, inexpensive and accurate method to detect male infertility, we have developed an in vitro test using simple incubation method. The basic for this test is to observe sperm motility with forward progression in an 8... analysis was found in an 8-h in vitro test whereas zona-free hamster egg penetration assay could not detect these differences. This study demonstrates that we have developed a simple, convenient inexpensive and accurate technique to detect male infertilityItem Metadata only The viability testing of frozen-thawed bovine embryo produced in vitro(Mahidol University. Mahidol University Library and Knowledge Center, 1993) Chainarong Tocharus; Kanok Pavasuthipaisit; Prasert Sobhon; Sukumal ChongthammakunItem Metadata only Studies of platelet abnormalities in thalassemia(Mahidol University. Mahidol University Library and Knowledge Center, 2023) Wittaya Tonsuwonnont; Udom Chantharaksri; Prawase Wasi; Suthat Fucharoenthe assessment of platelet function in vitro is subjected to minute changes in the platelet environment. This study was designed to define those functional abnormalities in thalassemia platelets and also to characterize those variables that contribute to theses... by Chemiluminescence, spectrophotometer, high performance liquid chromatography and radio immunoassay, respectively. Tests of platelet function were performed in the whole blood (WB), platelet-rich plasma (PRP) and washed platelet suspension (WPS). It was foundItem Metadata only Investigation of antimalarial principles from seeds of Amomum krervanh (Zingiberaceae)(Mahidol University. Mahidol University Library and Knowledge Center, 2024) Chongdee Nilanonta; Chachanat Thebtaranonth; Yodhathai Thebtaranonthbeen proposed for the remaining two compounds which are thought to be diterpenes. The above compounds ( with exception of the flavonoid ) give EC50 values in the range 10-5-10-8 g/ml. Besides in vitro testing, in vivo tests were also carried out... on myrtenol and one of the diterpenes which exhibited an in vitro EC50 value of 6.15 x 10-8 g/ml. However, in vivo test results are not yet conclusive due to problems concerning solubility of the compounds.Item Metadata only Sex determination of bovine embryos using H-Y antibody(Mahidol University. Mahidol University Library and Knowledge Center, 2024) Mayuva Areekijseree; Kanok Pavasuthipaisit; Prapee Sretarugsa; Yindee KitiyanantThe purpose of this study was to produce H-Y antibody from adult C57 BL/6 female mice, to test the titer and specificity of H-Y antibody by sperm cytotoxicity assay and to determine the sex of transferable stage of bovine embryos by using H-Y... classified as either H-Y positive (male embryo) or H-Y negative (female embryo). Moreover, the alternation of sex ratio during the developmental stage of embryo were investigated. The sex of 192 bovine embryos produced by in vitro fertilization wereItem Metadata only Mutagenic tests of steviol and stevioside(Mahidol University. Mahidol University Library and Knowledge Center, 2024) Sirirat Klongpanichapak; Chaivat Toskulkao; Thirayudh Glinsukon; Punya Temcharoen; Suntaree ApibalStevioside, a sweet-tasting diterpene glycoside derived from Stevia rebaudiana, and steviol, a product of enzymatic hydrolysis of stevioside, were tested for mutagenic activity in the in vitro Ames test (preincubation method) using Salmonella... the micronucleus test. Hamsters were orally administered with steviol or stevioside at a dose of 4 or 10 g/kg BW, respectively. Then after 24,30,48 or 72 h of treatment bone marrow samples were obtained and scored for MNPCEs. It was foundItem Metadata only Effects of some inhibitors on linamarin biosynthesis by cassava plantlets in tissue culture(Mahidol University. Mahidol University Library and Knowledge Center, 2024) Saipin Sanghirun; Montri Chulavatnatol; Tipaporn Limpaseni; Pichit Tosukhowongplantlets in tissue culture were used to test whether the plantlets could grow normally when linamarin biosynthesis is inhibited. To do so, potential inhibitors of enzyme involved in the biosynthesis were first tested in vitro using soluble enzyme and tissue... slices before final in vivo test in the cassava plantlets tissue culture. Five analogs of acetone cyanohydrin were found to completely inhibit the glucosyltransferase which catalyzed linamarin synthesis from acetone cyanohydrin. Pre-incubation experimentItem Metadata only Induction of sister chromatid exchanges by clastogenic effect of palmyrah palm (Borassus flabelliger)(Mahidol University. Mahidol University Library and Knowledge Center, 1987) Duangsmorn Maratana; Punya Temcharoen; Daoroong Kangwanpong; Chinvorn Promchainantalready described i.e. induction of malignant lymphomas immunosuppression and neurotoxicity in rats, in vitro clastogenicity on human lymphocyte and mutagenic effect on Ames test....Palmyrah (Borassus flabellifer) flour is consumed by people in certain tropical countries. Its extracts were tested for the induction of sister chromatid exchanges (SCEs) in human peripheral lymphocytes by BrdU-giemsa method in order to evaluateItem Metadata only Development of genus and species specific monoclonal antibodies against Schistosoma SP.from schistosoma mekongi Voge et al.,1978(Mahidol University. Mahidol University Library and Knowledge Center, 2024) Oraprapai Gajanandana; Vithoon Viyanant; Suchart Upatham; Prasert Sobhon; Maleya KruatrachueMonoclonal antibodies (MoAbs) directed against Schistosoma mekongi were produced by the in vitro fusion of the spleen cells, obtained from mice infected with S. mekongi and P3U1 myeloma cells. A total of 388 hybridoma clones were produced and grown... method. Initial characterization of monoclonal antibodies was tested by using immunoblotting assay against tegumental antigens of S. mekongi. The clear reaction bands were observed in 13 MoAb clones. Although most MoAbs have been found to bind to ratherItem Metadata only Prevalence of asymptomatic genital herpes in near-term pregnant women and anti-HSV-2 IgM response following first episode and recurrent diseases(Mahidol University. Mahidol University Library and Knowledge Center, 1988) Wanna Pumeechockchai; Chalobon Yoosook; Pantipa Sinarachatanantat Ramathibodi Hospital was investigated by using in vitro cultivation procedure. Various other methods for detectionof HSV antigens were also compared to this standard cell culture assay. These includes biotin - streptavidin enzyme-linked immunosorbent assay (B...-SA ELISA), immunoperoxidase staining of tissue smears and the staphylococcal coagglutination test. An attempt to detect anti-HSV-2 IgM was also carried out in cord blood samples obtained from the infants born to some of these mothers. Anit-HSV-2 Igm