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Item Metadata only Regulation and sequences of the light-responsive promoter of cyanobacterial ORF76 gene overlapping with the htpG terminator(Mahidol University. Mahidol University Library and Knowledge Center, 2023) Prae Plansangkate; Wipa Chungjatupornchai; Sakol Panyim; Prapon Wilairat; Witoon Tirasophonmonocistronic transcript. The promoter regions of ORF76 gene overlapped with the coding sequence and 3' end termination signal of htpG gene. Cell growth and morphology of the homologous ORF76-inactivated strains were not significantly different from those... transcript โปรโมเตอร์ของยีน ORF76 ทับซ้อนกับ coding sequence และ 3' end termination signal ของยีน htpG การเจริญเติบโตและลักษณะเซลล์ของ homologous ORF76-inactivated strainsไม่ต่างกับ wild type อย่างมีนัยสำคัญ อย่างไรก็ตาม homologous ORF76-inactivated strainsItem Metadata only A corpus-based study of antonym sequence in Japanese(Mahidol University. Mahidol University Library and Knowledge Center, 2018) Phornthip Nithithanawiwat; Isara Choosri; Pattama PatpongA corpus-based study of antonym sequencing in Japanese aimed: (1) to investigate antonym sequencing when co-occurring in one sentence; (2) to investigate the factors affecting antonym sequencing; (3) to examine whether the preferred ordering within... frequency patterns of the ordered antonym pairs in antonym sequences in Japanese follow the principle of markedness. It was found that 7 factors based on the principle of markedness influenced sequencing, namely positivity, phonology, morphology, magnitudeItem Metadata only Use of the predict-observe-explain teaching sequence for flow-based technique and new development of two flow systems for food safety and quality control(Mahidol University. Mahidol University Library and Knowledge Center, 2023) Saowapak Teerasong; Duangjai Nacapricha; Bhinyo Panijpan; Pintip Ruenwongsa; Nuanlaor RatanawimarnwongItem Metadata only Identification of molecular basis of [beta]-thalassemia/HbE gene in Thai population using synthetic oligonucleotide probes(Mahidol University. Mahidol University Library and Knowledge Center, 2023) Songsak Petmitr; Prapon Wilairat; Prawase Wasi; Montri Chulavatnatol; Sakol Panyim; Thanit Kusamran-thalassemic gene have been reported, including large sequence deletion, short sequence deletion or insertion, and single base substitution. The presence of large sequence deletion (619 b and 3.4 kb) were screened in 60 Thai patients with β-thalassemia/Hb...E using fragment length analysis, but none were detected. Single base substitution or insertion and short sequence deletion in β-globin gene of 30 Thai patients with β-thalassemia/HbE were screened by direct gel hybridization of BamH I fragmented DNAItem Metadata only Cloning and characterization of the alkaline protease gene (alpA) from Aspergillus oryzae(Mahidol University. Mahidol University Library and Knowledge Center, 2023) Supaporn Cheevadhanarak; Flegel, Timothy W.; Amaret Bhumiratana; Skorn Mongkolsuk; Holt, Geoffreyprobes based on the published cDNA sequence [Tatsumi et al., Agric. Biol. Chem. 52(1988) 1887-1888]. The entire nucleated sequence of the genomic clone obtained was determined. By comparison with the published cDNA sequence, it was found that Alp... was encoded by four exons of 314, 445, 89, and 351 bp. Three introns, which interrupted the coding sequence, were 50, 59, and 56 bp in length. The gene contains a typical TATA box 103 base pairs upstream, from the ATG start codon, and a consensusItem Metadata only Construction of synthetic genes for plasmodium falciparum dihydrofolate reductase-thymidylate synthase and Pneumocystic carinii dihydrofolate reductase(Mahidol University. Mahidol University Library and Knowledge Center, 2023) Phisit Prapunwattana; Yongyuth Yuthavong; Prapon Wilairat; Wichai SuttimoolBased on the known protein sequences, two synthetic genes were designed and constructed. One encoded Pneumocystis carinii dihydrofolate reductase (PcDHFR) and another encoded the bifunctional Plasmodium falciparum dihydrofolate reductasethymidylate... synthase (PfDHFR-TS). The synthetic P. falciparum DHFR-TS was first constructed as 3 separate domains: DHFR (PfDHFR), junction and TS (PfTS) domains and all domains were joined together to create the synthetic entire DHFR-TS sequence. DNA sequences of allItem Metadata only Resistance Plasmid Metagenomics in hospitalized patients' gut Microbiome(Mahidol University. Mahidol University Library and Knowledge Center, 2023) Tossawan Jitwasinkul; Chanwit Tribuddharat; Prapat Suriyaphol; Sithichoke TangphatsornruangI and Phi29 DNA polymerase. The 454 high-throughput sequencing (GS Junior) revealed DNA outputs from approximately 9 to 81 Mega base- pairs. By an integration of NCBI BLAST and Pfam, most predicted resistance proteins conferred the resistance to most... plasmid types were reported that linked with the discovered resistance genes, though resistance genes and plasmid replicons were not on the same sequences. From our findings, there were limitations on resistance gene sequencing coverage, becauseItem Metadata only Some biological aspects of the Anopheles dirus complex(Mahidol University. Mahidol University Library and Knowledge Center, 2023) Anan Poopityastaporn; Visut Baimai; Praneet Damrongpholsalivary gland polytene chromosome arm of species A, B, C, D and F of the An. dirus complex from natural populations in Thailand were presented. Structural conformation of heterokaryotypes and comparison of chromosome banding sequences revealed 10..., float length, and number of ridges on floats. Number of teeth per maxilla and length of maxillary tooth row of females of the 5 members of the An. dirus complex were studied. Species rank based on both characteristics, from the largest to the smallestItem Metadata only Molecular analysis of the factor mediating actin-based motility in Burkholderia species(Mahidol University. Mahidol University Library and Knowledge Center, 2023) Chayada Sitthidet; Sunee Korbsrisate; Pattarachai Kiratisin; Narusara ChantratitaBurkholderia pseudomallei เป็นแบคทีเรียแกรมลบซึ่งเป็นสาเหตุของโรคเมลิออยด์ แบคทีเรียชนิด นี้สามารถเคลื่อนที่ภายในและระหว่างเซลล์โดยอาศัย actin ของเซลล์ติดเชื้อที่เรียกว่า actin-based motility ขบวนการดังกล่าว เกิดจากการทำหน้าที่ของโปรตีน BimA ที่ผลิต...จากเชื้อ B. pseudomallei โปรตีนนี้นอกจากพบ ใน B. pseudomallei แล้วยังพบในเชื้อสายพันธุ์ใกล้เคียง ได้แก่ B. thailandensis and B. mallei ถึงแม้ว่าโปรตีน BimA ที่พบในเชื้อเหล่านี้มีส่วนประกอบของยีนที่แตกต่างกัน แต่สามารถกระตุ้นการเกิด actin-based motilityItem Metadata only Identification of RNA-processing abnormalities of the PKD1 gene in Thai families with autosomal dominant polycystic kidney disease(Mahidol University. Mahidol University Library and Knowledge Center, 2023) Wanna Thongnoppakhun; Prapon Wilairat; Pa-thai Yenchitsomanus; Mathurose Ponglikitmongkol; Chintana Sirinavinand polymerase chain reaction (RT-PCR) to selectively amplify the entire PKDI coding sequence from its mRNA transcripts, prepared from peripheral blood Iymphocyte, has successfully been developed in this study. The principle of this method is based on the use... is reiterated and at least three highly homologous genes are present at another region on the same chromosome. The attempts to isolate the entire PKDI coding sequence have never previously been successful, delaying the progression in analysis of PKDI mutations