18 results
Search Results
Now showing 1 - 10 of 18
Item Metadata only Nucleotide sequence analysis and strain improvement for high expression of penicillin G acylase gene from Bacillus megaterium UN-1(Mahidol University. Mahidol University Library and Knowledge Center, 2024) Kanchana Weeradechapon; Watanalai Panbangred; Vithaya Meevootisom; Chuenchit Boonchirdmegaterium UN-1, a high penicillin G acylase producer mutant of Bacillus megaterium ATCC 14945. Complete nucleotide sequence of pac gene was determined and found that the gene contained an open reading frame encoding for 802 amino acids with MW of about 92...-PAGE suggested that the penicillin G acylase of Bacillus megaterium UN-1 comprised of two subunits MW of about 23,000 Da and 57,000 Da. Therefore, this enzyme seem to be synthesized first as a single polypeptide and later being processed duringItem Metadata only Genetic manipulation of penicillin acylase in bacillus sp.(Mahidol University. Mahidol University Library and Knowledge Center, 2023) Suang Udomvaraphunt; Vithaya Meevootisom; Flegel, Timothy William; Amaret Bhumiratana; Sakol Panyim; Watanalai PanbangredLocalization of the Bacillus megaterium penicillin acylase (PAC) gene on plasmid pMLV101 was done by using the transposition inactivation technique. The PAC gene was found to be about 2.8 - 3.1 Kb in length. Partial digestion of pMLV101... of enzyme activety as E. coli which carried the plasmid pMLV101. The recombinant B. subtilis which carried plasmid pHBA33 produced PAC extracellularly, but, its activity was slightly lower than that of B. megaterium UN-1.Item Metadata only Conversion of bacterial penicillin-G acylase to cephalosporin-C acylase by protein engineering(Mahidol University. Mahidol University Library and Knowledge Center, 2003) Catleya Rojviriya; Jirundon Yuvaniyama; Pimchai Chaiyen; Vithaya MeevootisomItem Metadata only Studies on the purification and characterization of penicillin G acylase from Bacillus megaterium pBA 402/5(Mahidol University. Mahidol University Library and Knowledge Center, 2024) Ampa Sriinkaew; Kavi Ratanabanangkoon; Vithaya MeevootisomPenicillin G acylase is an enzyme which hydrolyzes benzylpenicillin to 6-aminopenicillanic acid, a key intermediate in the manufacturing of semisynthetic penicillins. Purification methods of extracellular penicillin G acylase from B. megaterium p...เอนไซม์เอนนิซิลลิน จี เอซิเลส เป็นเอนไซม์ที่ใช้ใน การผลิต 6-aminopenicillanic acid ซึ่งเป็นสารตัวกลาง ที่นำมาใช้ในขบวนการผลิตเพนนิซิลลินกึ่งสังเคราะห์ ได้ทำการ ศึกษาขบวนการทำเอนไซม์เพนนิซิลลิน จี เอซิเลส จากเชื้อ Bacillius megaterium pBA 402/5 ให้Publication Metadata only Cloning and expression of penicillin acylase genes from overproducing strains of Escherichia coli and Bacillus megaterium(1987-01-01) V. Meevootisom; J. R. Saunders; Mahidol University; University of LiverpoolPenicillin acylase genes from Escherichia coli 194, of an overproducing mutant (194-3) of this strain, and of a similar overproducing mutant of Bacillus megaterium UN1 were cloned in E. coli DH1 on the plasmid vector pACYC184. The sizes... in the original strains. The Bacillus penicillin acylase was produced intracellularly in E. coli recombinants, which is in contrast to the normal extracellular production of this enzyme in B. megaterium. Recombinant plasmids containing penicillin acylase genesPublication Metadata only High expression of the penicillin G acylase gene (pac) from Bacillus megaterium UN1 in its own pac minus mutant(2000-08-15) W. Panbangred; K. Weeradechapon; S. Udomvaraphant; K. Fujiyama; V. Meevootisom; Mahidol University; Osaka UniversityBy marker exchange mutagenesis, Bacillus megaterium strain UN-1 (Bm-UN1) was used to prepare a mutant strain B. megaterium UN-cat (Bm-UNcat) lacking the penicillin G acylase gene (pac). The pac gene from Bm-UN1 was subcloned into pTF6... and the resultant plasmid, pBA402, was introduced into Bm-UNcat and Bacillus subtilis. Bm-UNcat harbouring pBA402 produced high penicillin G acylase (PAC) activity of 13.7, 19.5 and 20.4 U ml1 at 24, 36 and 48 h of culture, respectively. This was two- to fivefoldPublication Metadata only Biodegradation of Bisphenol A by a Newly Isolated Bacillus megaterium Strain ISO-2 from a Polycarbonate Industrial Wastewater(2018-11-01) B. Suyamud; D. Inthorn; B. Panyapinyopol; P. Thiravetyan; South Carolina Commission on Higher Education; Mahidol University; King Mongkut s University of Technology Thonburi© 2018, Springer Nature Switzerland AG. A novel bacterium, Bacillus megaterium strain ISO-2, capable of the degradation of bisphenol A (BPA), was isolated from wastewater collected from a polycarbonate industry. The bacterium, which was grown...-MS) and liquid chromatography-mass spectrometry (LC-MS) analysis revealed several intermediates during the BPA degradation process by B. megaterium strain ISO-2. These intermediates were identified as 4-(2-hydroxypropan-2-yl)phenol, 4-isopropylphenolPublication Metadata only Improved X-ray diffraction from Bacillus megaterium penicillin G acylase crystals through long cryosoaking dehydration(2011-12-01) Catleya Rojviriya; Thunyaluck Pratumrat; Mark A. Saper; Jirundon Yuvaniyama; Mahidol University; University Michigan Ann ArborPenicillin G acylase from Bacillus megaterium (BmPGA) is currently used in the pharmaceutical industry as an alternative to PGA from Escherichia coli (EcPGA) for the hydrolysis of penicillin G to produce 6-aminopenicillanic acid (6-APA), a penamItem Metadata only Production of Polyhydroxybutyrate (PHB) by Novel Bacillus megaterium SWU01 Isolated from Activated Sludge(2022-11-01) Chuavong W.; Ponprateep S.; Ajawatanawong P.; Vatanavicharn T.; Mahidol Universitywere closely related to Bacillus genera. The comparison of PHB production from bacterial isolates showed that SWU01 strain isolated from the activated sludge exhibited the highest yield of PHB production. The cultivation of SWU01 stain in optimizationPublication Metadata only Characterization of two siderophores produced by Bacillus megaterium: A preliminary investigation into their potential as therapeutic agents(2020-10-01) Hataichanok Chuljerm; Manu Deeudom; Suthat Fucharoen; Francesca Mazzacuva; Robert C. Hider; Somdet Srichairatanakool; Agostino Cilibrizzi; Mahidol University; King's College London; Chiang Mai University© 2020 Elsevier B.V. Background: Microorganisms produce siderophores in order to scavenge iron from the environment and this study focuses on the characterization of the two siderophores secreted by Bacillus megaterium. The general biological