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    PublicationOpen Access
    Nuclear factor kappa B modulates apoptosis in the brain endothelial cells and intravascular leukocytes of fatal cerebral malaria
    (2013) Chuchard Punsawad; Yaowapa Maneerat; Urai Chaisri; Kwannan Nantavisai; Parnpen Viriyavejakul; Mahidol University. Faculty of Tropical Medicine. Department of Tropical Pathology
    in the brain of fatal CM. Methods: The expression of NF-κB p65 and cleaved caspase-3 in the brain of fatal P. falciparum malaria cases was investigated by immunohistochemistry. Histopathological features were analysed together with the correlations of NF-κB... p65 and cleaved caspase-3 expression. Results: NF-κB p65 activation and cleaved caspase-3 expression were significantly increased in the neurons, glial cells, vascular endothelial cells (ECs) and intravascular leukocytes of the brain in fatal CM
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    PublicationOpen Access
    Etlingera elatior Extract promotes cell death in B16 melanoma cells via down-regulation of ERK and Akt signaling pathways.
    (2017) Aungkana Krajarng; Malin Chulasiri; Ramida Watanapokasin; Mahidol University. Faculty of Pharmacy; Thammasat University. Chulabhorn International College of Medicine; Srinakharinwirot University. Faculty of Medicine. Department of Biochemistry
    Hoechst 33,342 and JC-1 staining. Flow cytometry using Annexin V/PI double staining assessed apoptosis, necrosis and viability. Caspase activity was detected by caspase activity kits. The expression of Bcl-2 family proteins, ERK and Akt signaling... of mitochondrial membrane potential, which are markers of apoptosis. Furthermore, the expression of Bim was increased instead of Bax and Bcl-2. The results also showed caspase-independent activity and the down-regulation of ERK and Akt signaling pathway
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    PublicationOpen Access
    Dengue 2 infection of HepG2 liver cells results in endoplasmic reticulum stress and induction of multiple pathways of cell death
    (2013) Chutima Thepparit; Khakpoor, Atefeh; Sarawut Khongwichit; Nitwara Wikan; Chanida Fongsaran; Pimjai Chingsuwanrote; Patcharee Panraksa; Smith, Duncan R; Mahidol University. Institute of Molecular Biosciences. Molecular Pathology Laboratory; Mahidol University. Center for Emerging and Neglected Infectious Diseases
    In response to DENV infection, HepG2 cells showed the induction of both the ER resident unfolded protein response as well as the Noxa/PUMA stress response pathways. Proteolytic activation of caspases 4, 7, 8 and 9 was observed as well as changes
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    PublicationOpen Access
    Liver changes in severe Plasmodium falciparum malaria: histopathology, apoptosis and nuclear factor kappa B expression.
    (2014-03-17) Parnpen Viriyavejakul; พรรณเพ็ญ วิริยเวชกุล; Vasant Khachonsaksumet; วสันต์ ขจรศักดิ์สุเมธ; Chuchard Punsawad; ชูชาติ พันธ์สวัสดิ์; Parnpen Viriyavejakul; Mahidol University. Faculty of Tropical Medicine. Department of Tropical Pathology.; Mahidol University. Center for Emerging and Neglected Infectious Diseases.
    histology was normal (the control group) (10 cases). The histopathology of the liver tissue was studied by routine histology method. Caspase-3 and nuclear factor kappa B (NF-κB) p65 expressions were determined using immunohistochemistry. RESULTS... died from P. falciparum malaria with hyperbilirubinaemia (total bilirubin (TB)≥ 51.3 μmol/L or 3 mg/dl) (12 cases), P. falciparum malaria without hyperbilirubinaemia (TB<51.3 μmol/L) (10 cases); and patients who died due to accidents, whose liver
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    PublicationOpen Access
    Aqueous extract of Thai medical Herbs (Phytoplex) Inhibits Cell Proliferation and Induces Apoptosis in human cervical cancer cell line (HeLa cells)
    (2018) Somjit Chaiwattanarungruengpaisan; Warunya Chakritbudsabong; Nattapat Rutjanavate; Rassameepen Phonarknguen; Ganokon Urkasemsin; Sasitorn Rungarunlert; สมจิตร ใช้วัฒนรุ่งเรืองไพศาล; วรัญญา ชาคริตบุษบง; ณัฐภัทร รุจจนเวท; รัศมีเพ็ญ โพธิ์นาคเงิน; กนกนอร เอื้อเกษมสิน; ศศิธร รุ่งอรุณเลิศ; Mahidol University. Faculty of Veterinary Science. The Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals (MoZWE); Mahidol University. Faculty of Veterinary Science. Department of Preclinic and Applied Animal Science; Mahidol University. Faculty of Veterinary Science. Department of Clinical Science and Public Health
    with a positive control (0.1% mitomycin C) and a negative control (0 μg/ml of Phytoplex) for 24, 48 and 72 h. Then, cell viability was evaluated using an MTT assay. The activities of caspase-3 (apoptosis marker) and Ki-67 (proliferation marker)†were... to 72 h of incubation peroid at high concentrations of Phytoplex. Moreover, Phytoplex inhibited HeLa cell proliferation with an IC50 value of 1,972.43, 1,230.10 and 1,317.67 μg/ml at 24, 48 and 72 h, respectively. The activity of caspase-3 of He
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    PublicationOpen Access
    NLRC4 and TLR5 each contribute to host defense in respiratory melioidosis.
    (2014-09-18) West, T. Eoin; Myers, Nicolle D.; Narisara Chantratita; นริศรา จันทราทิตย์; Wirongrong Chierakul; วิรงค์รอง เจียรกุล; Direk Limmathurotsakul; ดิเรก ลิ้มมธุรสกุล; Vanaporn Wuthiekanun; วรรณพร วุฒิเอกอนันต์; Miao, Edward A.; Hajjar, Adeline M.; Peacock, Sharon J.; Liggitt, H. Denny; Skerrett, Shawn J.; West, T. Eoin; Mahidol University. Faculty of Tropical Medicine. Mahidol-Oxford Tropical Medicine Research Unit.; Mahidol University. Faculty of Tropical Medicine. Department of Clinical Tropical Medicine.; Mahidol University. Faculty of Tropical Medicine. Department of Microbiology and Immunology.; Mahidol University. Faculty of Tropical Medicine. Department of Tropical Hygiene.
    , assembles a canonical caspase-1-dependent inflammasome in the cytoplasm that responds to flagellin (in mice) and type III secretion system components (in mice and humans). In a murine model of respiratory melioidosis, Tlr5 and Nlrc4 each contributed
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    PublicationOpen Access
    Effects of scabraside D on cell viability inhibition and apoptosis promotion of human hepatocellular carcinoma
    (2018) Rassameepen Phonarknguen; Thanakorn Rawangchue; Orathai Thongjuy; Kanjana Assawasuparerk; รัศมีเพ็ญ โพธิ์นาคเงิน; ธนกร ระวังชื่อ; อรทัย ทองจุ้ย; กาญจนา อัศวศุภฤกษ์; Mahidol University. Faculty of Veterinary Science. The Monitoring and Surveillance Center for Zoonotic Disease in Wildlife and Exotic Animals; Mahidol University. Faculty of Veterinary Science. The Center for Veterinary Diagnosis; Mahidol University. Faculty of Veterinary Science. Department of Pre-Clinical and Appiled Animal Science
    and 100 μg/mL significantly also induces morphological changes of apoptotic cell, including cell shrinkage, nuclear chromatin condensation and nuclear fragmentation. Quantitative real-time PCR shows that scabraside D up-regulated Bax and Caspase-3 while... caspase-3 แต่ลดการแสดงออกของยีน Bcl-2 ในเซลล์มะเร็งตับของมนุษย์ สรุปผลการทดลองพบว่า สคาบราไซด์ ดี สามารถยับยั้งการมีชีวิตของเซลล์มะเร็งตับมนุษย์และเหนี่ยวนำทำให้เกิดการตายของเซลล์แบบอะพอพโทซิส (apoptosis) อย่างมีนัยสำคัญทางสถิติ การศึกษาครั้งนี้แสดง
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    PublicationOpen Access
    Comprehensive proteomic analysis of white blood cells from chikungunya fever patients of different severities
    (2014) Nitwara Wikan; Sarawut Khongwichit; Weerawat Phuklia; Sukathida Ubol; Tipparat Thonsakulprasert; Montri Thannagith; Duangrudee Tanramluk; Atchara Paemanee; Suthathip Kittisenachai; Sittiruk Roytrakul; Smith, Duncan R; Mahidol University. Institute of Molecular Biosciences; Mahidol University. Center for Emerging and Neglected Infectious Diseases
    CHIKF samples respectively. Proteins with altered expression mapped predominantly to cellular signaling pathways (including toll-like receptor and PI3K-Akt signaling) although many other processes showed altered expression as a result of CHIKV... infection. Expression of proteins consistent with the activation of the inflammasome was detected, and quantitation of (pro)-caspase 1 at the protein and RNA levels showed an association with disease severity. Conclusions: This study confirms
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    PublicationOpen Access
    Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia
    (2011) Saranyoo Ponnikorn; Tasanee Panichakul; Kitima Sresanga; Chokdee Wongborisuth; Sittiruk Roytrakul; Suradej Hongeng; Sumalee Tungpradabkul; Mahidol University. Faculty of Science. Department of Biochemistry
    was analyzed using IMAC phosphoprotein isolation followed by LC-MS/MS detection. Decyder MS software was used to quantitate differentially expressed proteins in 3 patients and 2 normal donors. The differentially expressed proteins from HSCs/CD34+ cells were... in apoptotic cells including cytochrome C, caspase 6 and apoptosis inducing factors. Moreover, in the HSCs patients a significant increase was observed in a specific type of phosphoserine/threonine binding protein, which is known to act as an important
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    PublicationOpen Access
    Proteomic analysis of Chikungunya virus infected microgial cells
    (2012-04) Bizunesh Abere; Nitwara Wikan; Sukathida Ubol; Prasert Auewarakul; Atchara Paemanee; Suthathip Kittisenachai; Sittiruk Roytrakul; Duncan R. Smith; Mahidol University. Institute of Molecular Biosciences. Molecular Pathology Laboratory; Mahidol University. Faculty of Science. Department of Microbiology; Mahidol University. Center for Emerging and Neglected Infectious Disease.
    Chikungunya virus (CHIKV) is a recently re-emerged public health problem in many countries bordering the Indian Ocean and elsewhere. Chikungunya fever is a relatively self limiting febrile disease, but the consequences of chikungunya fever can include a long lasting, debilitating arthralgia, and occasional neurological involvement has been reported. Macrophages have been implicated as an important cell target of CHIKV with regards to both their role as an immune mediator, as well evidence pointing to long term viral persistence in these cells. Microglial cells are the resident brain macrophages, and so this study sought to define the proteomic changes in a human microglial cell line (CHME-5) in response to CHIKV infection. GeLC-MS/MS analysis of CHIKV infected and mock infected cells identified some 1455 individual proteins, of which 90 proteins, belonging to diverse cellular pathways, were significantly down regulated at a significance level of p<0.01. Analysis of the protein profile in response to infection did not support a global inhibition of either normal or IRES-mediated translation, but was consistent with the targeting of specific cellular pathways including those regulating innate antiviral mechanisms.