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Publication Open Access Preparing for introduction of a dengue vaccine: Recommendations from the 1st Dengue v2V Asia-Pacific Meeting(2011-11-28) Sai Kit Lam; Donald Burke; Maria Rosario Capeding; Chee Keong Chong; Laurent Coudeville; Jeremy Farrar; Duane Gubler; Sri Rezeki Hadinegoro; Jeffrey Hanna; Jean Lang; Han Lim Lee; Yee Sin Leo; Chan Quang Luong; Richard Mahoney; John Mcbride; Jorge Mendez Galvan; Lee Ching Ng; Suchitra Nimmannitya; Eng Eong Ooi; Donald Shepard; Jaco Smit; Rémy Teyssou; Laurent Thomas; Joseph Torresit; Pedro Vasconcelos; Dewa Nyoman Wirawan; Sutee Yoksan; Mahidol University. Institute of Molecular BiosciencesInfection with dengue virus is a major public health problem in the Asia-Pacific region and throughout tropical and sub-tropical regions of the world. Vaccination represents a major opportunity to control dengue and several candidate vaccines... are in development. Experts in dengue and in vaccine introduction gathered for a two day meeting during which they examined the challenges inherent to the introduction of a dengue vaccine into the national immunisation programmes of countries of the Asia-PacificPublication Open Access Responses of primary human nasal epithelial cells to EDIII-DENV stimulation: the first step to intranasal dengue vaccination(2016) Nattika Nantachit; Panya Sunintaboon; Sukathida Ubol; Mahidol University. Faculty of Science. Department of MicrobiologyBackground: About half of the world’s population are living in the endemic area of dengue viruses implying that a rapid-mass vaccination may be required. In addition, a major target of dengue vaccine are children, thus, a needle-free... administration is more attractive. These problems may be overcome by the alternative route of vaccination such as topical, oral and intranasal vaccination. Here, we investigated the possibility to deliver a dengue immunogen intranasally, a painless routePublication Open Access Comparison of full-length genomics sequences between dengue virus serotype 3, parental strain, and its derivatives, and B-cell epitopes prediction from envelope region(2013) Siriwattana Thaisonthi; Jundee Rabablert; Sutee Yoksan; Mahidol University. Institute of Molecular Biosciences; Mahidol University. Faculty of Science. Department of BiologyBiological markers are normally used to evaluate the candidate of live-attenuated dengue vaccines. D3V 16562 Vero 23 and D3V 16562 Vero 33 which were derivatives of D3V 16562, parental strain, showed the similar biological data. We used molecular... data and B-cell epitope make the derivatives attractive for the chimeric and peptide DENV vaccine development.Publication Open Access Effect of Thai medicinal plant extracts against dengue virus in vitro(2010-08-11T04:01:37Z) N Klawikkan; Veena Nukoolkarn; N Jirakanjanakit; S Yoksan; Chanpen Wiwat; วีณา นุกูลการ; จันทร์เพ็ญ วิวัฒน์; กฤษณ์ ถิระพันธุ์เมธี; Krit Thirapanmethee; Krit Thirapanmethee; Mahidol University. Faculty of Pharmacy. Department of Microbiology; Mahidol University. Faculty of Pharmacy. Department of Pharmacognosy; Mahidol University. Institute for Molecular Biosciences. Center for Vaccine Developmentbut distinct serotypes DENV 1, 2, 3, and 4. It is transmitted to people through the bite of mosquitoes Aedes aegypti and Aedes albopictus. It has been reported that dengue virus infects 50 to 100 million people annually. However, there is neither vaccine nor...Dengue hemorrhagic fever is one of the major public health problems in Thailand. The disease caused by the mosquito-borne dengue virus (DENV). DENV is the member of the Flavivirus genus, Flaviviridae family. There are four genetically relatedPublication Open Access Plaque reduction neutralization antibody test does not accurately predict protection against dengue infection in Ratchaburi cohort, Thailand(2014) Chukiat Sirivichayakul; Arunee Sabchareon; Kriengsak Limkittikul; Sutee Yoksan; Mahidol University. Faculty of Tropical Medicine. Department of Tropical PediatricsBackground: The plaque reduction neutralization test (PRNT) is currently the best and most widely accepted approach to measuring virus-neutralizing and protective antibodies to dengue virus, and in assessing the immunogenicity of a dengue vaccine.... However, the correlation between presence of dengue-neutralizing antibody and protection from infection is not absolute. Findings: In a cohort study in Ratchaburi Province, Thailand, 48 subjects with serologically confirmed symptomatic dengue infectionPublication Open Access Neutralizing dengue antibody in pregnant Thai women and cord blood.(2015-02-06) Kriangsak Khamim; เกรียงศักดิ์ คำอิ่ม; Weerawan Hattasingh; วีรวรรณ หัตถสิงห์; Ananda Nisalak; Jaranit Kaewkungwal; จรณิต แก้วกังวาล; Fernandez, Stefan; Butsaya Thaisomboonsuk; บุษยา ไทยสมบูรณ์สุข; Krisana Pengsaa; กฤษณา เพ็งสา; Usa Thisyakorn; อุษา ทิสยากร; Weerawan Hattasingh; Mahidol University. Faculty of Tropical Medicine. Department of Tropical Pediatrics.; Mahidol University. Faculty of Tropical Medicine. Centre of Excellence for Biomedical & Public Health Informatics (BIOPHICS); Mahidol University. Faculty of Tropical Medicine. Department of Tropical Hygiene.these goals requires future dengue prevention strategies that will employ both potential vaccines and sustainable vector-control measures. Maternally transferred dengue antibody is an important factor in determining the optimal age for dengue vaccination...BACKGROUND: The WHO 'Global Strategy for Dengue Prevention and Control, 2012-2020' addresses the growing need for the treatment of dengue, and targets a 25% reduction in morbidity and 50% in mortality (using 2010 estimates as baseline). AchievingPublication Open Access Epidemiological and Molecular Characterization of Dengue Virus Circulating in Bhutan, 2013-2014(2015-08-21) Sangay Zangmo; Chonticha Klungthong; Piyawan Chinnawirotpisan; Srisurang Tantimavanich; Nathamon Kosoltanapiwat; Butsaya Thaisomboonsuk; Kelzang Phuntsho; Sonam Wangchuk; In-Kyu Yoon; Stefan Fernandez; Mahidol University. Faculty of Medical Technology. Department of Clinical Microbiology and Applied TechnologyDengue is one of the most significant public health problems in tropical and subtropical countries, and is increasingly being detected in traditionally non-endemic areas. In Bhutan, dengue virus (DENV) has only recently been detected and limited... information is available. In this study, we analyzed the epidemiological and molecular characteristics of DENV in two southern districts in Bhutan from 2013–2014. During this period, 379 patients were clinically diagnosed with suspected dengue, of whom 119 (31Publication Open Access Dengue virus infection induces expansion of a CD14(+)CD16(+) monocyte population that stimulates plasmablast differentiation.(2014-07-09) Kwissa Marcin; Nakaya, Helder I.; Nattawat Onlamoon; Wrammert Jens; Villinger Francois; Perng, Guey Chuen; Sutee Yoksan; Kovit Pattanapanyasat; Kulkanya Chokephaibulkit; Ahmed Rafi; Pulendran Bali; Mahidol University. Faculty of Medicine Siriraj Hospital. Insitute of Molecular Biosciences; Mahidol University. Faculty of Medicine Siriraj Hospital. Department of PediatricsDengue virus (DENV) infection induces the expansion of plasmablasts, which produce antibodies that can neutralize DENV but also enhance disease upon secondary infection with another DENV serotype. To understand how these immune responses... are generated, we used a systems biological approach to analyze immune responses to dengue in humans. Transcriptomic analysis of whole blood revealed that genes encoding proinflammatory mediators and type I interferon-related proteins were associated with highPublication Open Access Accuracy of AccessBio Immunoglobulin M and Total Antibody Rapid Immunochromatographic Assays for the Diagnosis of Acute Scrub Typhus Infection(2010-02) Blacksell,Stuart D.; Kemajittra Jenjaroen; Rattanaphone Phetsouvanh; Vanaporn Wuthiekanun; วรรณพร วุฒิเอกอนันต์; Day, Nicholas P. J.; Newton, Paul N.; Ching, Wei-Mei; Blacksell,Stuart D.; Mahidol University. Faculty of Tropical Medicine. Mahidol University-Oxford Tropical Medicine Research Unit (MORU)Using archived samples, we assessed the diagnostic capacity of a rapid immunochromatographic test (ICT) for the detection of Orientia tsutsugamushi IgM and total antibodies to aid with the diagnosis of acute scrub typhus infection in febrile patients in Laos. The sensitivity and the specificity of the ICT for the detection of IgM were 96.8% (121/125 samples; 95% confidence interval [CI], 92.1 to 99.1%) and 93.3% (98/105 samples; 95% CI, 86.7 to 97.3%), respectively. For the detection of total antibodies, the sensitivity was 97.6% (122/125 samples; 95% CI, 93.1 to 99.5%), but the specificity was much lower, at 71.4% (75/105 samples; 95% CI, 61.8 to 79.8%).Publication Open Access Delayed antibody dependent enhancement of low passage dengue virus 4 isolates(2015) Nitwara Wikan; Sirikwan Libsittikul; Sutee Yoksan; Prasert Auewarakul; Duncan R. Smith; Mahidol University. Institute of Molecular BiosciencesBackground: The concept of antibody dependent enhancement (ADE) of dengue virus (DENV) infection is a cornerstone of our current understanding of dengue pathogenesis, although some questions as to the mechanism remain, particularly in regards