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Item Open Access Seroprevalence of toxoplasma gondii using sabin feldman dye test in children and young adults in Bangphae district, Ratchaburi province, Thailand(Mahidol University. Mahidol University Library and Knowledge Center, 2022) Sugai, Misaki, 1978-; Kriengsak Limkittikul; Salin Sirinam; Weerawan Hattasingh; Aongart Mahittikron, gender, and living subdistricts. T. gondii IgG was tested using the Sabin-Feldman dye test. The seroprevalence of T. gondii was 2.8% and 4.2% in 2012 and 2015, respectively. In 2015, the highest seropositivity of 18/285 (6.3%) was found in ages 14-23Publication Open Access Biosynthesis of Silver Nanoparticles Using Orange Peel Extract for Application in Catalytic Degradation of Methylene Blue Dye(2021) Cathleen Simatupang; Vinod K Jindal; Ranjna Jindal; Mahidol University. Faculty of Engineering. Department of Civil and Environmental Engineering; Mahidol University. Faculty of Engineering. Department of Chemical Engineering. This study focused on the biosynthesis of AgNPs using orange peel extract (OPE), optimization of process conditions, and application in catalytic degradation of methylene blue (MB) dye used in the textile industry. A central composite design in response... wavelength of 403.8 nm in UV-vis spectra, and minimum particle size of 12.9 nm. In addition, peak absorbance and SPR wavelength appeared to be related to the size of the AgNPs. A full-factorial design for the catalytic degradation of MB dyeItem Open Access Development of an Immunofluorescent Staining Assay for Histodiagnosis of Pythiosis(2014) Ruchuros Inkomlue; Tassanee Lerksuthirat; Angsana Keeratijarut; Tassanee Lowhnoo; Wanta Yingyong; Koset Pinpradap; Pitak Santanirand; Noppadol Larbcharoensub; Theerapong Krajaejun; Mahidol University. Faculty of Medicine, Ramathibodi Hospital. Research Center; Mahidol University. Faculty of Science. Multidisciplinary Unitwith vascular pythiosis (n=3) and other fungal infections (2 Candida albicans, 1 Aspergillus flavus, and 1 Fusarium sp). All infected-tissue blocks from pythiosis patients were tested positive, while those from patients with other mycoses were testedPublication Open Access Kinetic Adsorption of Hazardous Methylene Blue from Aqueous Solution onto Iron-Impregnated Powdered Activated Carbon(2019) Athit Phetrak; Sirirat Sangkarak; Sumate Ampawong; Suda Ittisupornrat; Doungkamon Phihusut; Mahidol University. Faculty of Tropical Medicine. Department of Social and Environmental Medicine; Mahidol University. Faculty of Tropical Medicine. Department of Tropical Pathology; Department of Environmental Quality Promotion. Environmental Research and Training Center; Chulalongkorn University. Environmental Research InstituteIn this study, iron-impregnated powderedactivated carbon (Fe-PAC) prepared using chemical co-precipitation techniques was used as an adsorbent for methylene blue (MB) removal in a batch experiment. The analysis of transmission electron microscopy, scanning electron microscopy with energy dispersive spectroscopy showed that iron oxide particle was substantially distributed into the surface of the adsorbent, suggesting that Fe-PAC was successfully synthesized. The results showed that fast and efficient adsorption of MB by Fe-PAC was achieved, witha relative short contact time of 10 min and MB adsorption capacity of 51 mg/g. The kinetic adsorption of MB on Fe-APC adsorbent was well described by a pseudo-second-order model. Concurrently, the analysis of intraparticle diffusion model suggests that intraparticle diffusion is not the only rate-limiting step of MB molecules adsorption by Fe-PAC adsorbent. The elevated temperature conditions also improved the removal efficiency of MB. Thermodynamic parameters exhibited by the MB adsorption process onto Fe-PAC were endothermic and spontaneous. The findings of the present work indicate that Fe-PAC can be a potentially effective adsorbent for MB removal in wastewater due to its fast and efficient MB adsorption, and separation in wastewater treatment systems.Publication Open Access ระยะเวลาที่มีผลต่อความคงตัวของสารเรืองแสงในการย้อมเซลล์ที่ถูกตรึง เพื่อวิเคราะห์ด้วยเทคนิค Flow Cytometry(2559) สิรภพ วงษ์เนียม; Sirapope Wongniam; มหาวิทยาลัยมหิดล. คณะวิทยาศาสตร์. หน่วยเครื่องมือกลาง; Mahidol University. Faculty of Science. Central Instrument FacilityThe success of a flow cytometric analysis depends on the stability of fluorescent dyes during preserving time. To gain more details in the stability of fluorescent dyes, the fixative cell lines (MCF-7, HuCCA-1 and HT1080 cells) and white bloodPublication Open Access Sensitivity and specificity of PS/AA-modified nanoparticles used in malaria detection(2012-11) Raweewan Thiramanas; Kulachart Jangpatarapongsa; Udom Asawapirom; Pramuan Tangboriboonrat; Duangporn Polpanich; Center for Innovation Development and Technology Transferand affinity binding via streptavidin (SA) and biotin interaction, were employed. The optimum ratio of Ab to NPs used in each immobilization procedure and the latex agglutination test based on the reaction between Ab conjugated NPs and malaria patient plasmaPublication Open Access The removal of colors in non-carbonated soft drinks using granular activated carbon for coliform bacteria detection by sanitary indicator medium(2009) Vatanasomboon, P; Pisit Vatanasomboon; Rojanavipart, P; Piangchan Rojanavipart; Luksamijarulkul, P; Pipat Luksamijarulkul; พิศิษฐ์ วัฒนสมบูรณ์; เพียงจันทร์ โรจนวิภาต; พิพัฒน์ ลักษมีจรัลกุล; Srisantisang, N; Mahidol University. Faculty of Public Health. Department of Environmental Health Science; Mahidol University. Faculty of Public Health. Department of Biostatistics; Mahidol University. Faculty of Public Health. Department of Microbiologyfor blue color-tone. The validity of coliform bacteria detection by SI medium for these removed-color soft drinks when compared with the standard MPN method were sensitivity value, specificity value and efficiency of test of more than 80%. Thus, GAC can...ที่สุดสำหรับเครื่องดื่มสีฟ้า เมื่อหาค่าความถูกต้อง (validity) ของการตรวจโคลิฟอร์มแบคทีเรียด้วย SI medium ในเครื่องดื่มที่ถูกกำจัดสีออกด้วยสภาวะที่เหมาะสมที่สุด เปรียบเทียบกับวิธีมาตรฐาน MPN ได้ค่า sensitivity, specificity และ efficiency of test มากกว่า 80Publication Open Access Quantitative evaluation of cellular intensity in cytologic staining over difference time period of post air-dried smear in canine mammary gland tumor(2018) Jeerasak sri-in; Pattita Ruayaree; Panpaga Sangsuriya; Panop wilainam; Parin Suwannaprapha; จีรศักดิ์ ศรีอินทร์; ปทิตตา รวยอารี; พรรณพงา เเสงสุริยะ; ภานพ วิไลนาม; ปริญ สุวรรณประภา; จุฬาลงกรณ์มหาวิทยาลัย. คณะเภสัชศาสตร์; มหาวิทยาลัยมหิดล. คณะสัตวแพทยศาสตร์. ภาควิชาปรีคลินิกและสัตวศาสตร์ประยุกต์Diagnostic cytology is an initial laboratory testing to diagnose various types of cancer, infections and inflammatory diseases. The collection and processing of cytological specimens are very important to produce good quality specimensPublication Open Access Cytotoxicity evaluation of Clinacanthus nutans through dimethylthiazol diphenyltetrazolium bromide and neutral red uptake assays.(2016-01) Suwanna Korsuwannawong; สุวรรณา ก่อสุวรรณวงศ์; La‑Ongthong Vajrabhaya; ละอองทอง วัชราภัย; Suwanna Korsuwannawong; Mahidol University. Faculty of Dentistry. Research OfficeObjectives: The aim of this study was to compare the results of dimethylthiazol diphenyltetrazolium bromide (MTT) and neutral red uptake (NRU) assays of Clinacanthus nutans cytotoxicity. Materials and Methods: Mouse fibroblast (L929) cells were exposed to 0.01%, 0.1%, 0.25%, and 0.5% (W/V) C. nutans in a 96-cluster -well-culture plate for 24 h. The cell viability after exposure to C. nutans was determined by MTT and NRU assays in separate tissue culture plates. The two assays were compared through an intra‑class correlation coefficient (ICC) analysis. Results: No significant differences in cytotoxicity were noted between the two assays ( P > 0.05). The ICC values for agreement between two assays for the negative and positive control groups and C. nutans concentrations of 0.01%, 0.1%, 0.25%, and 0.5% were 0.84, 0.83, 0.77, 0.68, 0.74, and 0.71, respectively. Conclusion: In general, the MTT and NRU assays performed similarly, exhibiting moderate to good correlation for the evaluation of the cytotoxicity of C. nutans .Publication Open Access FlexiChip package: an universal microarray with a dedicated analysis software for high-thoughput SNPs detection linked to anti-malarial drug resistance.(2009-10-15) Steenkeste, Nicolas; Dillies, Marie-Agnès; Khim, Nimol; Sismeiro, Odile; Chy, Sophy; Lim, Pharath; Crameri, Andreas; Bouchier, Christiane; Mercereau-Puijalon, Odile; Beck, Hans-Peter; Mallika Imwong; มัลลิกา อิ่มวงศ์; Dondorp, Arjen M.; Socheat, Duong; Rogier, Christophe; Coppée, Jean- Yves; Ariey, Frédéric; Steenkeste, Nicolas; Mahidol University. Faculty of Tropical MedicineBACKGROUND: A number of molecular tools have been developed to monitor the emergence and spread of anti-malarial drug resistance to Plasmodium falciparum. One of the major obstacles to the wider implementation of these tools is the absence of practical methods enabling high throughput analysis. Here a new Zip-code array is described, called FlexiChip, linked to a dedicated software program, which largely overcomes this problem. METHODS: Previously published microarray probes detecting single-nucleotide polymorphisms (SNP) associated with parasite resistance to anti-malarial drugs (ResMalChip) were adapted for a universal microarray FlexiChip format. To evaluate the overall sensitivity of the FlexiChip package (microarray + software), the results of FlexiChip were compared to ResMalChip microarray, using the same extension probes and with the same PCR products. In both cases, sequence results were used as gold standard to calculate sensitivity and specificity. FlexiChip results obtained with a set of field isolates were then compared to those assessed in an independent reference laboratory. RESULTS: The FlexiChip package gave results identical to the ResMalChip results in 92.7% of samples (kappa coefficient 0.8491, with a standard error 0.021) and had a sensitivity of 95.88% and a specificity of 97.68% compared to the sequencing as the reference method. Moreover the method performed well compared to the results obtained in the reference laboratories, with 99.7% of identical results (kappa coefficient 0.9923, S.E. 0.0523). CONCLUSION: Microarrays could be employed to monitor P. falciparum drug resistance markers with greater cost effectiveness and the possibility for high throughput analysis. The FlexiChip package is a promising tool for use in poor resource settings of malaria endemic countries.