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Publication Open Access Occult Metastases in Negative Sentinel Lymph Node of Invasive Breast Cancer Discovered by Serial Section in Ramathibodi Hospital(2009) Orathai Thangnapakorn; Sansanee Wongwaisayawan; Panuwat Lertsithichai; Sasivimol Rattanasiri; อรทัย ตั้งนภากร; ศันสนีย์ วงศ์ไวศยวรรณ; ภาณุวัฒน์ เลิศสิทธิชัย; ศศิวิมล รัตนสิริ; Mahidol University. Faculty of Medicine Ramathibodi Hospital. Department of Pathology; Mahidol University. Faculty of Medicine Ramathibodi Hospital. Department of Surgery; Mahidol University. Faculty of Medicine Ramathibodi Hospital. Section for Clinical Epidemiology and BiostatisticsBackground: Sentinel lymph node (SLN) biopsy is an established method for evaluating axillary metastases in breast cancer. This study was designed to ascertain whether serial sections with haematoxylin and eosin stain could significant improve... were examined by serial sections at 25 microns intervals at each level with haematoxylin and eosin stain. Result: Occult metastases were detected by serial sectioning in 4 (8%) out of 50 breast cancer patients. One patient had macrometastasis, twoPublication Open Access Morphology and histology of the adult Paramphistomum gracile Fischoeder, 1901.(2013) Busaba Panyarachun; Arin Ngamniyom; Prasert Sobhon; Panat Anuracpreeda; Mahidol University. Kanchanaburi Campus. Agricultural Science; Mahidol University.Faculty of Science. Department of Anatomy; Mahidol University.Division of Agricultural Science.-sections were stained with hematoxylin and eosin, and examined. The body surface and longitudinal section of P. gracile were also assessed using scanning electron microscopy. In this species, the anterior sucker and posterior sucker (acetabulum) werePublication Open Access Mast cell activation in the skin of Plasmodium falciparum malaria patients.(2015-02-07) Panop Wilainam; ภานพ วิไลนาม; Rungrat Nintasen; Parnpen Viriyavejakul; พรรณเพ็ญ วิริยเวชกุล; Parnpen Viriyavejakul; Mahidol University. Faculty of Tropical Medicine. Department of Tropical Pathology.; Mahidol University. Faculty of Veterinary Science. Department of Preclinic and Applied Animal Science.cases. Normal skin tissues from 29 cases served as controls. Pre- and post-treatment tissues were included. Histopathological changes of the skin were evaluated using haematoxylin and eosin stain. MCs were investigated using toluidine blue stainingPublication Open Access Effect of L-cysteine on Chilled carp (Cyprinus carpio) semen qualities.(2013) Kan Kledmanee; Somrat Taweedet; Prawporn Thaijongruk; Panida Chanapiwat; Kampon Kaeoket; Mahidol University. Faculty of Veterinary Science. Department of Clinical Sciences and Public Health. Semen LaboratoryThe aim of the present study was to study the effects of L-cysteine on chilled carp (Cyprinus carpio) semen qualities. Pooled semen samples were prepared from eight fish, and divided into five groups according to the concentrations of L-cysteine as follows: 0 (T1), 0.5 (T2), 1.0 (T3), 1.5 (T4) and 2 (T5) mM. The sperm motility, duration of sperm motility and sperm viability were evaluated at 0, 12, 24, 48, 72 hours after chilled storage. Comparing between treatment and control groups, the percentage of sperm motility, duration of sperm motility and the percentage of sperm viability in the treatment groups were significantly higher than the control group (p < 0.05). Considering over a period of time after chilled storage, modified Kurokura’s extender plus L-cysteine groups were able to maintain carp semen qualities (motility, duration of motility and viability) up to 24 hour. Comparing all concentrations of L-cysteine, at 24 hour after chilled storage, the optimal concentration of L-cysteine was found at 1 mM. In conclusion, supplementation of L-cysteine at 1 mMol in modified Kurokura’s extender can be recommended for carp semen chilled storage.Publication Open Access Severe congenital nemaline myopathy with primary pulmonary lymphangiectasia: unusual clinical presentation and review of the literature(2015) Jariya Waisayarat; Chinnawut Suriyonplengsaeng; Chaiyos Khongkhatithum; Mana Rochanawutanon; Mahidol University. Faculty of Medicine Ramathibodi Hospital. Department of PathologyIntroduction: Nemaline myopathy is a rare genetic muscle disorder defined by the presence of nemaline rods in the muscle fibre sarcoplasm. Congenital nemaline myopathy is the most serious form of the disease’s spectrum. Case presentation: The affected newborn has no spontaneous movement, fractures at birth and respiratory insufficiency. The present case was a Thai male, floppy at birth with fractures of both humeri and femurs and ventilator-dependent respiration. The patient developed bilateral chylothorax two weeks later and died at the age of 6 weeks. Whole-body postmortem examination with informed consent and genetic analysis of ACTA1 mutation were performed. A skeletal muscle biopsy examined by light and transmission electron microscopy showed the features of nemaline myopathy. ACTA 1 heterozygous missense mutation (c.1127G > C) was identified. Histological examination of both lungs revealed primary pulmonary lymphangiectasia. Conclusion: To the best of our knowledge, congenital nemaline myopathy with primary pulmonary lymphangiectasia causing bilateral chylothrax has never been previously reported. Considering chylothorax as a poor prognostic index and an unusual clinical presentation of severe congenital NM are proposed.Publication Open Access The Effect of adding L-Cysteine in Modified Kenny's extender on the quality of chilled stallion semen at 5oC(2013) Panithi Sukho; Hiran Juwarahawong; Slitwan Sangiam; Kampon Kaeoket; Mahidol University. Faculty of Veterinary Science. Dapartment of Clinical Sciences and Public Health DepartmentThe aim of this study was to investigate the effect of supplementation of L-Cysteine in Modified Kenny's extender on chilled stallion semen quality at 5oC. Semen samples were collected from 3 stallions by artificial vagina and centrifugation to remove seminal plasma and diluted with Modified Kenny's extender to 50 x 106 sperm/ml. Semen samples (n=10 ejaculates) were divided into 3 portions, the first portion as the control (KE), the second portion was added with L-Cysteine 200 μmol/l (KEC200) and added L-Cysteine 400 μmol/l in the last portion (KEC400). In all samples, the percentage of progressive motility, viability and acrosome integrity were evaluated at 0, 24, 48 and 72 h after storage at 5oC. There were a significant (P < 0.05) lower percentage of dead spermatozoa in group KE200 than other groups at 24 h. There was a significantly lower percentage of progressive motility in KEC 400 compared toother groups at 48 h (P < 0.05). In addition, there was a tendency (P = 0.1) of lower percentage of dead spermatozoa in group KE200 than other groups at 48 h. At 72 h, comparing all groups, a higher percentage of viability in groups KEC200 and KEC400 was found, but not statistically significant. In conclusion, Addition of L-cysteine at a concentration of 200 μmol/l to the Modified Kenney's extender tend to improve the stallion chilled semen progressive motility, dead/alive, viability and acrosome integrity during chilled storage at 5oC for 48 h.Publication Open Access The effect of adding L-Cysteine in Modified Kenny's extender on the quality of chilled stallion semen at 5oC(2013) Panithi Sukho; Hiran Juwarahawong; Slitwan Sangiam; Kampon Kaeoket; Mahidol University. Faculty of Veterinary Science. Department of Clinical Sciences and Public Health. Semen LaboratoryThe aim of this study was to investigate the effect of supplementation of L-Cysteine in Modified Kenny's extender on chilled stallion semen quality at 5oC. Semen samples were collected from 3 stallions by artificial vagina and centrifugation to remove seminal plasma and diluted with Modified Kenny's extender to 50 x 106 sperm/ml. Semen samples (n=10 ejaculates) were divided into 3 portions, the first portion as the control (KE), the second portion was added with L-Cysteine 200 μmol/l (KEC200) and added L-Cysteine 400 μmol/l in the last portion (KEC400). In all samples, the percentage of progressive motility, viability and acrosome integrity were evaluated at 0, 24, 48 and 72 h after storage at 5oC. There were a significant (P < 0.05) lower percentage of dead spermatozoa in group KE200 than other groups at 24 h. There was a significantly lower percentage of progressive motility in KEC 400 compared toother groups at 48 h (P < 0.05). In addition, there was a tendency (P = 0.1) of lower percentage of dead spermatozoa in group KE200 than other groups at 48 h. At 72 h, comparing all groups, a higher percentage of viability in groups KEC200 and KEC400 was found, but not statistically significant. In conclusion, Addition of L-cysteine at a concentration of 200 μmol/l to the Modified Kenney's extender tend to improve the stallion chilled semen progressive motility, dead/alive, viability and acrosome integrity during chilled storage at 5oC for 48 h.Publication Open Access Trypanosoma evansi infection in mice and sow: cryopreservation of T. evansi, its infectivity and subsequent clinical signs and pathological findings(2011) Siriporn Tantawet; Bastiaan Willemse; Piyanant Taweethavornsawat; Kampon Kaeoket; Mahidol University. Faculty of Veterinary Science. Department of Clinical Science and Public HealthItem Open Access คู่มือปฎิบัติงาน เรื่อง การเตรียมสไลด์เนื้อเยื่อเพื่องานทางจุลพยาธิวิทยาช่องปาก คณะทันตแพทยศาสตร์ มหาวิทยาลัยมหิดล(2562) ดุสิต บุมาลี; มหาวิทยาลัยมหิดล. คณะทันตแพทยศาสตร์. ภาควิชาพยาธิวิทยาช่องปากและแม็กซิลโลเฟเซียลPublication Open Access Severe falciparum malaria treated with artesunate complicated by delayed onset haemolysis and acute kidney injury(2015) Katherine Plewes; Haider, Md Shafiul; Kingston, Hugh W F; Yeo, Tsin W; Aniruddha Ghose; Hossain, Md Amir; Dondorp, Arjen M; Turner, Gareth D H; Anstey, Nicholas M; Mahidol University. Faculty of Tropical Medicine. Mahidol-Oxford Tropical Medicine Research UnitBackground: Severe falciparum malaria may be complicated by haemolysis after parasite clearance, however the mechanisms remain unclear. Recent reports describe a pattern of delayed onset haemolysis among non-immune travellers with hyperparasitaemia treated with intravenous artesunate, termed post-artesunate delayed haemolysis (PADH). The occurrence and clinical impact of PADH following severe malaria infections in areas of unstable transmission are unknown. Case: A 45-year-old Bangladeshi male was initially admitted to a local hospital with severe falciparum malaria complicated by hyperparasitaemia and treated with intravenous artesunate. Twenty days from his first presentation he was readmitted with delayed onset haemolytic anaemia and acute kidney injury. Multiple blood transfusions and haemodialysis were required. Renal biopsy revealed acute tubular injury and haem pigment nephropathy. His haemoglobin and renal function recovered to baseline after 62 days from his second admission. Discussion: This case highlights the differential diagnosis of post-malaria delayed onset haemolysis, including the recently described syndrome of post-artemisinin delayed haemolysis. The pathophysiology contributing to acute kidney injury in this patient and the limited treatment options are discussed. Conclusions: This report describes PADH complicated by acute kidney injury in an adult patient living in a malaria hypoendemic region who subsequently required blood transfusions and haemodialysis. This case emphasizes the importance of routine follow up of haemoglobin and renal function in artesunate-treated patients who have recovered from severe malaria.