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Publication Open Access The use of fermented feather meal for replacement fish meal in the diet of Oreochromus Niloticus(2008-06) Chumlong Arunlertaree; Chutinthorn Moolthongnoi; Mahidol University. Faculty of Environment and Natural Resource StudiesThe suitability of fermented feather meal (FFM) as a replacement of dietary fish meal protein in the diet for tilapia was evaluated. Five diets were formulated in which fish meal (FM) in the control diet (diet 1) was replaced by FFM... poorest than those fed all other diets at the end of experimental periods. The results of the present study indicated that fermented feather meal could be used at 25 % up to 50 % of the diet level for Oreochromis niloticus without compromising growthPublication Open Access Epidemiology of bacteremia caused by uncommon non-fermentative gram-negative bacteria(2013) Pinyo Rattanaumpawan; Prapassorn Ussavasodhi; Pattarachai Kiratisin; Nalinee Aswapokee; Mahidol University. Faculty of Medicine Siriraj Hospital. Division of Infectious Diseases and Tropical MedicineBackground: Prevalence of bacteremia caused by non-fermentative gram-negative bacteria (NFGNB) has been increasing over the past decade. Although many studies have already investigated epidemiology of NFGNB bacteremia, most focused only on commonPublication Open Access Impacts of pH, temperature and pretreatment method on biohydrogen production from organic wastes by sewage microflora(2014) Jaruwan Wongthanate; Kittibodee Chinnacotpong; Madsamon Khumpong; Mahidol University. Faculty of Environment and Resource StudiesBiohydrogen production could be generated from organic wastes: food and beverage processing wastewater, restaurant food waste and raw starch waste. Fermentative hydrogen production from food and beverage processing wastewater by sewage microflora... characteristics were crucial to dark-fermentative hydrogen production. Pretreatment methods (methanogenic inhibitor, sterilization, sonication and acidification) on restaurant food waste and raw starch waste to enhance biohydrogen production were also investigatedPublication Open Access Flavoring agent from Salacca(2004-06) Walla Tungrugsasut; Vimol Srisukh; Jongoramon Wisamitanan; Petcharat Suksawat; Wilaiwan Thongbainoi; Mahidol University. Faculty of Pharmacy. Department of Food Chemistryinto 8 characteristic ones : mellow, salacca, sweet, sour, astringent, fermented, alcohol-like, and piercing. Varying combinations of distillates, 95% ethanol and concentrate exhibited similar desirable aroma to the fresh Salacca juice.Publication Open Access Large-scale production and antiviral efficacy of multi-target double-stranded RNA for the prevention of white spot syndrome virus (WSSV) in shrimp(2015) Thitiporn Thammasorn; Pakkakul Sangsuriya; Watcharachai Meemetta; Saengchan Senapin; Sarocha Jitrakorn; Triwit Rattanarojpong; Vanvimon Saksmerprome; Mahidol University. Faculty of Science. Center of Excellence for Shrimp Molecular Biology and BiotechnologyRNA yields as compared to the use of Luria Bertani (LB) broth. Co-culture expression was conducted under glycerol feeding fed-batch fermentation. Estimated yield of multi-WSSV dsRNA (μg/mL culture) from the fed-batch process was 30 times higher thanPublication Open Access การสำรวจสารปนเปื้อนทางเคมีในอาหารของงานกีฬามหาวิทยาลัยแห่งประเทศไทย ครั้งที่ 33(2551) ดวงใจ มาลัย; สุวัฒน์ ศรีสรฉัตร์; Duangjai Malai; Suwat Srisorrachatr; ดวงใจ มาลัย; มหาวิทยาลัยมหิดล. คณะสาธารณสุขศาสตร์. ภาควิชาโภชนวิทยา, sodium hydrosulfite, sulfite, formalin, and pesticide, respectively. As the result of this inspection, borax contaminated food were Chinese sausage, fermented mango, and seasoned preserved mango. Salicylic acid was found in a fermented bamboo shoot... sample. Bleaching agent were found in bean sprout, shrimp, squid, raw mango, and fermented mango, wonton sheets and fish balls. Formalin was found in straw mushrooms and sausage. Pesticide were found in onion, swamp cabbage, guava, fried dried-snakeItem Open Access คู่มือปฏิบัติงาน การตรวจหาตัวชี้วัดคุณภาพน้้าทางแบคทีเรียและไวรัส(2559) กรรณิการ์ ป้อมบุบผา; มหาวิทยาลัยมหิดล. คณะสาธารณสุขศาสตร์. ภาควิชาจุลชีววิทยาPublication Open Access The Effect of Oolong Tea Consumption on Postprandial Triglyceride Levels: A Randomized, Double-blind, Placebo-controlled Crossover Study(2015) Akkarach Bumrungpert; Rewadee Chongsuwat; เอกราช บำรุงพืชน์; เรวดี จงสุวัฒน์; Mahidol University. Faculty of Public Health. Department of NutritionOolong tea is produced by the semi-fermentation of the fresh leaves of Camellia sinensis. The major bioactive compound in oolong tea is oolong tea polymerized polyphenol (OTPP). Previous studies found that oolong tea reduces the absorption of fatPublication Open Access Comparative analysis of sugarcane bagasse metagenome reveals unique and conserved biomass-degrading enzymes among lignocellulolytic microbial communities(2015) Wuttichai Mhuantong; Varodom Charoensawan; Pattanop Kanokratana; Sithichoke Tangphatsornruang; Verawat Champreda; Mahidol University. Faculty of Science. Department of BiochemistryBackground: As one of the most abundant agricultural wastes, sugarcane bagasse is largely under-exploited, but it possesses a great potential for the biofuel, fermentation, and cellulosic biorefinery industries. It also provides a uniquePublication Open Access Effective enhancement of Pseudomonas stutzeri D-phenylglycine aminotransferase functional expression in Pichia pastoris by co-expressing Escherichia coli GroEL-GroES(2012) Kanidtha Jariyachawalid; Poramaet Laowanapiban; Vithaya Meevootisom; Suthep Wiyakrutta; Mahidol University. Faculty of Science. Department of MicrobiologyBackground: D-phenylglycine aminotransferase (D-PhgAT) of Pseudomonas stutzeri ST-201 catalyzes the reversible stereo-inverting transamination potentially useful in the application for synthesis of D-phenylglycine and D-4-hydroxyphenylglycine using L-glutamate as a low cost amino donor substrate in one single step. The enzyme is a relatively hydrophobic homodimeric intracellular protein difficult to express in the soluble functionally active form. Over-expression of the dpgA gene in E. coli resulted in the majority of the D-PhgAT aggregated into insoluble inclusion bodies that failed to be re-natured. Expression in Pichia pastoris was explored as an alternative route for high level production of the D-PhgAT. Results: Intracellular expression of the codon-optimized synthetic dpgA gene under the PAOX1 promoter in P. pastoris resulted in inactive D-PhgAT associated with insoluble cellular fraction and very low level of D-PhgAT activity in the soluble fraction. Manipulation of culture conditions such as addition of sorbitol to induce intracellular accumulation of osmolytes, addition of benzyl alcohol to induce chaperone expression, or lowering incubation temperature to slow down protein expression and folding rates all failed to increase the active D-PhgAT yield. Co-expression of E. coli chaperonins GroEL-GroES with the D-PhgAT dramatically improved the soluble active enzyme production. Increasing gene dosage of both the dpgA and those of the chaperones further increased functional D-PhgAT yield up to 14400-fold higher than when the dpgA was expressed alone. Optimization of cultivation condition further increased D-PhgAT activity yield from the best co-expressing strain by 1.2-fold. Conclusions: This is the first report on the use of bacterial chaperones co-expressions to enhance functional intracellular expression of bacterial enzyme in P. pastoris. Only two bacterial chaperone genes groEL and groES were sufficient for dramatic enhancement of functionally active D-PhgAT expression in this yeast. With the optimized gene dosage and chaperone combinations, P. pastoris can be attractive for intracellular expression of bacterial proteins since it can grow to a very high cell density which is translated into the higher volumetric product yield than the E. coli or other bacterial systems.