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    PublicationOpen Access
    FlexiChip package: an universal microarray with a dedicated analysis software for high-thoughput SNPs detection linked to anti-malarial drug resistance.
    (2009-10-15) Steenkeste, Nicolas; Dillies, Marie-Agnès; Khim, Nimol; Sismeiro, Odile; Chy, Sophy; Lim, Pharath; Crameri, Andreas; Bouchier, Christiane; Mercereau-Puijalon, Odile; Beck, Hans-Peter; Mallika Imwong; มัลลิกา อิ่มวงศ์; Dondorp, Arjen M.; Socheat, Duong; Rogier, Christophe; Coppée, Jean- Yves; Ariey, Frédéric; Steenkeste, Nicolas; Mahidol University. Faculty of Tropical Medicine
    of practical methods enabling high throughput analysis. Here a new Zip-code array is described, called FlexiChip, linked to a dedicated software program, which largely overcomes this problem. METHODS: Previously published microarray probes detecting single...-nucleotide polymorphisms (SNP) associated with parasite resistance to anti-malarial drugs (ResMalChip) were adapted for a universal microarray FlexiChip format. To evaluate the overall sensitivity of the FlexiChip package (microarray + software), the results
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    PublicationOpen Access
    Biomarkers for Refractory Lupus Nephritis: A Microarray Study of Kidney Tissue.
    (2015-06) Pornpen Tantivitayakul; พรเพ็ญ ตันติวิทยากุล; Thitima Benjachat; ธิติมา เบญจชาติ; Pumipat Tongyoo; ภูมิพัฒน์ ทองอยู่; Poorichaya Somparn; ภูริชญา สมภาร; Nattiya Hirankarn; ณัฏฐิยา หิรัญกาญจน์; Santitham Prom-On; สันติธรรม พรหมอ่อน; Prapaporn Pisitkun; ประภาพร พิสิษฐ์กุล; Asada Leelahavanichkul; อัษฎางศ์ ลีฬหวนิชกุล; Yingyos Avihingsanon; ยิ่งยศ อวิหิงสานนท์; Natavudh Townamchai; ณัฐวุฒิ โตวนำชัย; Yingyos Avihingsanon; ยิ่งยศ อวิหิงสานนท์; Mahidol University. Faculty of Dentistry. Department of Oral Microbiology; Mahidol University. Faculty of Medicine, Ramathibodi Hospital. Department of Medicine
    Pathology Society (ISN/RPS) classification III/IV were recruited. Twenty-three kidney tissue samples were used for RNA microarray analysis, while the remaining 44 samples were used for validation by real-time polymerase chain reaction (PCR) gene expression
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    PublicationOpen Access
    Global gene expression profiling of Plasmodium falciparum in response to the anti-malarial drug pyronaridine
    (2011-08-18) Kanyanan Kritsiriwuthinan; กัญญนันทน์ กฤษศิริวุฒินันท์; Sastra Chaotheing; Shaw, Philip J.; Chayaphat Wongsombat; Porntip Chavalitshewinkoon-Petmitr; พรทิพย์ เพ็ชรมิตร; Sumalee Kamchonwongpaisan; สุมาลี กำจรวงศ์ไพศาล; Sumalee Kamchonwongpaisan; Mahidol University. Faculty of Tropical Medicine. Department of Protozoology
    , and parasites were harvested from culture after 4 and 24 hours exposure. Global transcriptional changes effected by drug treatment were investigated using DNA microarrays. RESULTS: After a 4 h drug exposure, PN induced a greater degree of transcriptional.... However, PN appears to exert a more rapid response than CQ. The faster action of PN may explain why PN is more efficacious than CQ, particularly against CQ resistant isolates. In agreement with several other microarray studies of drug action
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    PublicationOpen Access
    Determination of whole transcription profiles and specific pathways in invasive ductal breast carcinoma
    (2013-05-15) Pasra Arnutti; ภัสรา อาณัติ; Manas Kotepui; มนัส โคตรพุ้ย; Wichitra Asanprakit; Phaibul Punyarit; Porntip Chavalitshewinkoon-Petmitr; พรทิพย์ ชวลิตรชีวินกุล เพ็ชรมิตร; Talabporn Harnroongroj; ตรับพร หาญรุ่งโรจน์; Songsak Petmitr; ทรงศักดิ์ เพ็ชรมิตร; Songsak Petmitr; Mahidol University. Faculty of Tropical Medicine. Department of Tropical Nutrition & Food Science.; Mahidol University. Faculty of Tropical Medicine. Department of Molecular Tropical Medicine & Genetics.; Mahidol University. Faculty of Tropical Medicine. Department of Protozoology.
    Breast cancer is the most common cancer affecting women worldwide including Thailand. Whole transcription profiles of invasive ductal breast carcinoma (IDC) obtained by oligonucleotide microarray should lead to a better understanding
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    PublicationOpen Access
    An integrated lab-on-chip for rapid identification and simultaneous differentiation of tropical pathogens.
    (2014-07-31) Tan, Jeslin J. L.; Capozzoli, Monica; Sato, Mitsuharu; Wanitda Watthanaworawit; วนิตดา วัฒนวรวิทย์; Ling, Clare L.; Mauduit, Marjorie; Malleret, Benoît; Grüner, Anne-Charlotte; Tan, Rosemary; Nosten, François H.; Snounou, Georges; Rénia, Laurent; Ng, Lisa F. P.; Rénia, Laurent; Ng, Lisa F. P.; Mahidol University. Faculty of TropicalMedicine. Mahidol-Oxford Tropical Medicine Research Unit. Shoklo Malaria Research Unit
    diagnosis will be crucial in patient management and in the control of these diseases. In this study, a microfluidic lab-on-chip integrating multiplex molecular amplification and DNA microarray hybridization was developed for simultaneous detection... virus; and a positive 85.0% agreement and a specificity of 100% for Dengue virus serotype 3 with reference methods conducted on the samples. Results suggested the practicality of an amplification microarray-based approach in a field setting for high
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    PublicationOpen Access
    Genomic characterization of non-O1, non-O139 Vibrio cholerae reveals genes for a type III secretion system
    (2005-03-01) Michelle Dziejman; Davide Serruto; Vincent C. Tam; Derek Sturtevant; Pornphan Diraphat; Shah M. Faruque; M. Hasibur Rahman; John F. Heidelberg; Jeremy Decker; Li Li; Kate T. Montgomery; George Grills; Raju Kucherlapati; John J. Mekalanos; Mahidol university. Faculty of Public Health
    relationship to other pathogenic V. cholerae. Comparative genomic microarray analysis of four pathogenic non-O1, non-O139 strains indicates that these strains are quite divergent from O1 and O139 strains. Genomic sequence analysis of a non-O1, non-O139 strain
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    PublicationOpen Access
    Transcriptional profiles of Burkholderia pseudomallei reveal the direct and indirect roles of Sigma E under oxidative stress conditions
    (2014) Siroj Jitprasutwit; Ong, Catherine; Niramol Juntawieng; Wen Fong Ooi; Hemsley, Claudia M.; Paiboon Vattanaviboon; Titball, Richard W.; Tan, Patrick; Sunee Korbsrisate; Mahidol University. Faculty of Medicine Siriraj Hospital. Department of Immunology
    Background: Burkholderia pseudomallei, the causative agent of melioidosis, is a Gram-negative bacterium widely distributed in soil and water in endemic areas. This soil saprophyte can survive harsh environmental conditions, even in soils where herbicides (containing superoxide generators) are abundant. Sigma factor E (σE) is a key regulator of extra-cytoplasmic stress response in Gram-negative bacteria. In this study, we identified the B. pseudomallei σE regulon and characterized the indirect role that σE plays in the regulation of spermidine, contributing to the successful survival of B. pseudomallei in stressful environments. Results: Changes in the global transcriptional profiles of B. pseudomallei wild type and σE mutant under physiological and oxidative stress (hydrogen peroxide) conditions were determined. We identified 307 up-regulated genes under oxidative stress condition. Comparison of the transcriptional profiles of B. pseudomallei wild type and σE mutant under control or oxidative stress conditions identified 85 oxidative-responsive genes regulated by σE, including genes involved in cell membrane repair, maintenance of protein folding and oxidative stress response and potential virulence factors such as a type VI secretion system (T6SS). Importantly, we identified that the speG gene, encoding spermidine-acetyltransferase, is a novel member of the B. pseudomallei σE regulon. The expression of speG was regulated by σE, implying that σE plays an indirect role in the regulation of physiological level of spermidine to protect the bacteria during oxidative stress. Conclusion: This study identified B. pseudomallei genes directly regulated by σE in response to oxidative stress and revealed the indirect role of σE in the regulation of the polyamine spermidine (via regulation of speG) for bacterial cell protection during oxidative stress. This study provides new insights into the regulatory mechanisms by which σE contributes to the survival of B. pseudomallei under stressful conditions.
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    PublicationOpen Access
    Increased alpha-defensin expression is associated with risk of coronary heart disease: a feasible predictive inflammatory biomarker of coronary heart disease in hyperlipidemia patients
    (2016) Yaowapa Maneerat; Kriengchai Prasongsukarn; Surachet Benjathummarak; Wilanee Dechkhajorn; Urai Chaisri; Mahidol University. Faculty of Tropical Medicine. Department of Tropical Pathology
    and the development of CHD, and whether it was a useful predictive indicator for CHD risk. Methods: First, DNA microarray analysis was performed on peripheral blood mononuclear cells (PBMCs) from Thai control, hyperlipidemia and CHD male patients (n = 7). Gene
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    PublicationOpen Access
    PPBP and DEFA1/DEFA3 genes in hyperlipidaemia as feasible synergistic inflammatory biomarkers for coronary heart disease.
    (2017) Yaowapa Maneerat; Kriengchai Prasongsukarn; Surachet Benjathummarak; Wilanee Dechkhajorn; Mahidol University. Faculty of Tropical Medicine. Department of Tropical Pathology; Mahidol University. Faculty of Tropical Medicine. Center of Excellence for Antibody Research; Pramongkutklao Hospital and College of Medicine
    cells using DNA microarrays. Eight selected genes expressed only in H and CHD groups were validated by real-time quantitative reverse transcription PCR and plasma protein determination. Results: α-defensin (DEFA1/DEFA3), pro-platelet basic protein
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    PublicationOpen Access
    Artemisinin resistance in Plasmodium falciparum is associated with an altered temporal pattern of transcription
    (2011-08-03) Mok, Sachel; Mallika Imwong; มัลลิกา อิ่มวงศ์; Mackinnon, Margaret J.; Sim, Joan; Ramadoss, Ramya; Yi, Poravuth; Mayfong Mayxay; Kesinee Chotivanich; เกศินี โชติวานิช; Liong, Kek-Yee; Russell, Bruce; Socheat, Duong; Newton, Paul N.; Day, Nicholas P.J.; White, Nicholas J.; Preiser, Peter R.; Nosten, François; Dondorp, Arjen M.; Bozdech, Zbynek; Dondorp, Arjen M.; Mahidol University. Faculty of Tropical Medicine. Department of Molecular Tropical Medicine and Genetics; Mahidol University. Faculty of Tropical Medicine. Mahidol-Oxford Tropical Medicine Research Unit (MORU)
    throughout the world. To identify key features associated with the delayed parasite clearance phenotype, we employed DNA microarrays to profile the physiological gene expression pattern of the resistant isolates. RESULTS: In the ring and trophozoite