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Publication Open Access การเปรียบเทียบการวัดพื้นที่แผล 3 วิธี: โฟโตชอป อิมเมจเจ และวิสิเทรค(2561) นิโรบล กนกสุนทรรัตน์; พิศสมัย อรทัย; ทัศนีย์ จันทร์อ่อน; สุรเวช น้ำหอม; Nirobol Kanogsunthornrat; Pisamai Orathai; Tatsanee Junorn; Surawej Numhom; มหาวิทยาลัยมหิดล. คณะแพทยศาสตร์โรงพยาบาลรามาธิบดี. โรงเรียนพยาบาลรามาธิบดีwounds assessed from photographs, the wound sizes measured with Photoshop CS3 Extended were not different from those measured with ImageJ, and those from wound tracing measured with Visitrak.™ The results of this study suggest that, for accurate area...This comparative study aimed at comparing wound surface area measurement methods between wound tracing and wound photography and using Visitrak™ as a reference measurement device. Their sizes were measured using Photoshop CS3 Extended and ImagePublication Open Access ความตรงของวิธีการวัดพื้นที่หน้าตัดปากแผล(2557) ทัศนีย์ จันทร์อ่อน; นิโรบล กนกสุนทรรัตน์; พิศสมัย อรทัย; Tatsanee Junorn; Nirobol Kanogsunthorntrat; Pisamai Orathai; มหาวิทยาลัยมหิดล. คณะแพทยศาสตร์โรงพยาบาลรามาธิบดี. โรงเรียนพยาบาลรามาธิบดีThis descriptive study aimed at investigating the validity of wound surface area measurement methods by placing a transparent plasters on the wound surface area and scanning it as a computer image file before calculating its size using the Adobe... Photoshop CS3 Extended and Image J . A wound measurement device called the VisitrakTM was used as a reference tool. The subjects were 30 open wounds of patients who were treated at Ramathibodi Hospital from June to September 2012. The overall differencesPublication Open Access Using polypropylene mesh graft for soft-tissue reconstruction in internal hemipelvectomy: a case report(2012) Apichat Asavamongkolkul; Saranatra Waikakul; Mahidol University. Faculty of Medicine, Siriraj Hospital. Department of Orthopaedic SurgeryWe report the case of a patient with chondrosarcoma involving the right pelvis and contralateral pubic area in a 45-year-old male who underwent an extensive internal hemipelvectomy without bony reconstruction. We demonstrate the technique of using polypropylene mesh graft for soft-tissue reconstruction. Follow-up at 7.5 years showed a good oncological and functional outcome.Publication Open Access Quinoline-based clioquinol and nitroxoline exhibit anticancer activity inducing FoxM1 inhibition in cholangiocarcinoma cells(2015-04-08) Waraporn Chan-on; Nguyen Thi Bich Huyen; Napat Songtawee; Wilasinee Suwanjang; Supaluk Prachayasittikul; Virapong Prachayasittikul; Mahidol University. Faculty of Medical Technology. Department of Clinical Microbiology and Applied Technology; Mahidol University. Faculty of Medical Technology. Center of Data Mining and Biomedical Informaticsevaluated using the colorimetric 3-(4,5-dimethylthiazol-2yl)-5-(3-carboxymethoxyphenyl)-(4-sulfophenyl)-2H-tetrazolium (MTS assay). Colony formation and cell migration affected by CQ and NQ were investigated using a clonogenic and a wound healing assayPublication Open Access Three phase bone scintigraphy in evaluating revascularization and bone formation of natural biomaterial bone graft by using Tc99m MDP in rabbits(2015) Somkiat Huaijantug; Paranee Yatmark; Somsak Wattananit; Phingphol Charoonrut; Thonnapong Thongpraparn; Rujaporn Chanachai; Mahidol University. Faculty of Veterinary Science. Department of Clinical Sciences and Public Health; Mahidol University. Faculty of Veterinay Science. Department of Pre-Clinical and Apply Animal Science; Mahidol University. Faculty of Medicine Siriraj Hospital. Section of Nuclear MedicineThree phase bone scintigraphy (TPBS) examined the viability of bone graft, revascularization, and bone formation using single photon emission computed tomography (SPECT) and the intravenous injection of 99M Technetium methylenediphosphonate (99MTc-MDP). Seven New Zealand White rabbits, aged 8 months had osteotomy of ulna performed, the left ulna substituted for natural biomaterial bone graft, containing gelatin sponge and hyaluronic acid, and the right ulna was replaced by vascularized autogenous bone graft. A post-operative scanning of bone using TPBS was done at 7 days and weekly, at week 1, 2, 4, 6, 8, 12 to evaluate revascularization and bone formation of natural biomaterial bone graft comparing with autogenous bone graft. The results demonstrated that the perfusion index and RCP of natural biomaterial bone graft and the autogenous bone graft in phases 1, 2, and 3 had no statistically significant difference when compared in between each week. The study also showed that, the ratio of the mean counts/pixel of the natural biomaterial bone graft in phase 3 had slightly lower than autogenous bone graft. This study provided model through which TPBS could evaluate revascularization and bone formation in this model. Natural biomaterial bone graft may be the same and as good a revascularization as autogenous bone graft although the bone formation of autogenous bone graft still has more efficiency than natural biomaterial bone.Publication Open Access Three phase bone scintigraphy in evaluating revascularization and bone formation of natural biomaterial bone graft by using Tc99m MDP in rabbits(2015) Somkiat Huaijantug; Paranee Yatmark; Somsak Wattananit; Phingphol Charoonrut; Thonnapong Thongpraparn; Rujaporn Chanachai; Mahidol University. Faculty of Veterinary Science; Mahidol University. Faculty of Medicine Siriraj HospitalThree phase bone scintigraphy (TPBS) examined the viability of bone graft, revascularization, and bone formation using single photon emission computed tomography (SPECT) and the intravenous injection of 99M Technetium methylenediphosphonate (99MTc-MDP). Seven New Zealand White rabbits, aged 8 months had osteotomy of ulna performed, the left ulna substituted for natural biomaterial bone graft, containing gelatin sponge and hyaluronic acid, and the right ulna was replaced by vascularized autogenous bone graft. A post-operative scanning of bone using TPBS was done at 7 days and weekly, at week 1, 2, 4, 6, 8, 12 to evaluate revascularization and bone formation of natural biomaterial bone graft comparing with autogenous bone graft. The results demonstrated that the perfusion index and RCP of natural biomaterial bone graft and the autogenous bone graft in phases 1, 2, and 3 had no statistically significant difference when compared in between each week. The study also showed that, the ratio of the mean counts/pixel of the natural biomaterial bone graft in phase 3 had slightly lower than autogenous bone graft. This study provided model through which TPBS could evaluate revascularization and bone formation in this model. Natural biomaterial bone graft may be the same and as good a revascularization as autogenous bone graft although the bone formation of autogenous bone graft still has more efficiency than natural biomaterial bone.Publication Open Access Association between histological grading, Ki-67, AgNOR, KIT expression pattern and survival time in a dog with mast cell Tumour: A case report(2020) Ploen Nganthavee; Paitoon Srimontri; Tanit Kasantikul; Namphung Suemanotham; เพลิน งานทวี; ไพฑูรย์ ศรีมนตรี; ฐานิต กสานติกุล; น้ำผึ้ง สื่อมโนธรรม; Mahidol University. Faculty of Veterinary ScienceA 13-year-old entire male Thai Ridgeback dog presented to Prasu-Arthorn Animal Hospital, Faculty of Veterinary Science, Mahidol University with a mass on the caudal right flank. Fine needle aspiration (FNA) revealed round cells with intracytoplasmic granules consistent with mast cell tumour (MCT). Surgical excision was performed. Histological grading revealed poorly-differentiated, high grade MCT with 18 mitotic figures per 10 high power-fields (HPFs) and KIT staining was consistent with KIT pattern II (focal or stippled cytoplasmic staining); both of which are associated with poor survival. This contrasted with the better survival time implicated by low argyrophilic nucleolar organiser region (AgNOR) and Ki-67 scores, which were all below the cut-off values, suggesting low cellular proliferation. A chemotherapy protocol of vinblastine and prednisolone was subsequently commenced. Distant recurrence at the neck occurred at 67 days, confirmed to be MCT by histopathology. The dog later died, with a total survival time of 90 days from diagnosis. The actual survival time closely aligned with the estimated survival time based on histological grading. With few multivariate survival analyses available, this case demonstrates the use of AgNOR, Ki-67, and KIT localisation to complement histological grading for further studies to compare different variables in a clinical setting in Thailand.