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Publication Open Access ค่าทางโลหิตวิทยาและเคมีคลินิกในเลือดแมวที่มีสุขภาพดี(2557) ปริยากร จายคำ; พงษ์พันธุ์ สุวรรณชาติ; ศุภกิจ บัวมาศ; อรุณี แจ้งแสงทอง; วราภรณ์ แก้วคงจันทร์; วชิร ตระกูลชัยศรี; ชุติเพ็ญ บูรณะสินทรัพย์; มหาวิทยาลัยมหิดล. คณะสัตวแพทยศาสตร์blood chemistry value in our laboratory, the authors collect the blood from healthy feline that visited Prasuarthon hospital, Faculty of Veterinary Science, Mahidol University. Hematology and clinical blood chemistry were analyzed by automatedPublication Open Access ค่าทางโลหิตวิทยาและเคมีคลินิกในเลือดแมวที่มีสุขภาพดี(2557) ปริยากร จายคำ; พงษ์พันธุ์ สุวรรณชาติ; ศุภกิจ บัวมาศ; อรุณี แจ้งแสงทอง; วราภรณ์ แก้วคงจันทร์; วชิร ตระกูลชัยศรี; ชุติเพ็ญ บูรณะสินทรัพย์; มหาวิทยาลัยมหิดล. คณะสัตวแพทยศาสตร์. ห้องปฏิบัติการโรงพยาบาลสัตว์ประศุอาทร; มหาวิทยาลัยมหิดล. คณะสัตวแพทยศาสตร์. ภาควิชาปรีคลินิกและสัตวศาสตร์ประยุกต์blood chemistry value in our laboratory, the authors collect the blood from healthy feline that visited Prasuarthon hospital, Faculty of Veterinary Science, Mahidol University. Hematology and clinical blood chemistry were analyzed by automated hematologyPublication Open Access Feline Leukemia Virus (FeLV) in Captive Wild Felids in Thailand during 2004-2005(2010) Siriporn Tangsudjai; Supunsa Malidang; Rassmeepen Phonarknguen; Roschong Boonyarittichaikit; Rattapan Pattanarangsan; Ruangrat Buddhirongawatr; Kridsada Chaichoun; Mahidol University. Faculty of Veterinary SciencePublication Open Access Retinal lesion in a Toxoplasma gondii seropositive Bengal tiger (Panthera tigris) in a private zoo, Kanchanaburi Province(2015) Areerat Kongcharoen; Ruangrat Buddhirongawatr; Siriporn Tungsudjai; Nareerat Sangkhachai; Poonyapat Sedwisai; Thekhawet Weluwanarak; Tatiyanuch Chamsai; Mahidol University. Faculty of Veterinary Science. Prasu-Arthorn Veterinary Teaching Hospital; Mahidol University. Faculty of Veterinay Science. Monitoring and Surveillance Center for Zoonotic Disease in Wildlife and Exotic Animalsof them appeared in normal condition and had no signs of illness. Their blood was tested negative for feline leukemia virus, feline immunodeficiency virus, feline infectious peritonitis virus and Toxoplasma gondii using molecular detection of viral antigenPublication Open Access Retinal lesion in a Toxoplasma gondii seropositive Bengal tiger (Panthera tigris) in a private zoo, Kanchanaburi Province(2015) Areerat Kongcharoen; Ruangrat Buddhirongawatr; Siriporn Tungsudjai; Nareerat Sangkhachai; Poonyapat Sedwisai; Thekhawet Weluwanarak; Tatiyanuch Chamsai; Mahidol University. Faculty of Veterinary Scienceof them appeared in normal condition and had no signs of illness. Their blood was tested negative for feline leukemia virus, feline immunodeficiency virus, feline infectious peritonitis virus and Toxoplasma gondii using molecular detection of viralPublication Open Access Using of CRFK and HeLa Dual Cell-Culture System to Isolate Canine Distemper Virus(2020) Jarupha Taowan; Pruksa Julapanthong; Wanvisa Surarith; Natthapat Ketchim; Chalisa Mongkolphan; Kridsada Chaichoun; Mahidol University. Faculty of Veterinary Science; Mahidol University. Faculty of Veterinary Science. The Monitoring and Surveillance Center for Zoonotic Disease in Wildlife and Exotic Animals; Mahidol University. Faculty of Veterinary Science. Internal Medicine Unit Prasu Arthorn Animal Hospitalinfections. Many different types of cultured cells can be used for isolation of distemper virus, which provides the different outcome. This study aimed to evaluate the efficacy of dual cell culture system, Crandell-Rees feline kidney (CRFK) and HeLa cells... เซลล์เพาะเลี้ยงหลายประเภทสามารถใช้ในการแยกไวรัสไข้หัดสุนัขซึ่งให้ผลลัพธ์ที่แตกต่างกัน การศึกษานี้มีวัตถุประสงค์เพื่อปะเมินประสิทธิภาพของระบบการเพาะเลี้ยงเซลล์คู่โดยใช้เซลล์ Crandell-Rees feline kidney (CRFK) และเซลล์ HeLa สำหรับการแยกเชื้อไวรัส ในการPublication Open Access L-carnitine supplemented extender improves cryopreserved-thawed cat epididymal sperm motility(2014-06) Manee-In S; Parmornsupornvichit S; Kraiprayoon S; Tharasanit T; Chanapiwat P; Kaeoket K; Mahidol University. Faculty of Veterinary Science. Department of Clinical Sciences and Public Health MedicineCryopreservation of epididymal sperm is an effective technique to preserve genetic materials of domestic cats and wild felids when they unexpectedly die. However, this technique inevitably causes detrimental changes of cryopreserved-thawed spermatozoa, for example, by physical damage and excessive oxidative stress. L-carnitine is an antioxidant that has been used to improve sperm motility in humans and domestic animals. This study aimed to investigate the effects of L-carnitine on cat epididymal sperm quality following cryopreservation and thawing. After routine castration, cauda epididymides were collected from 60 cat testes. The epididymal spermatozoa from 3 cauda epididymides were pooled as 1 replicate. Spermatozoa samples (16 replicates) were examined for spermatozoa quality and then randomly divided into 4 groups: 0 mM L-carnitine (control), 12.5 mM, 25 mM and 50 mM L-carnitine. The sperm aliquots were then equilibrated and conventionally frozen. After thawing, sperm motility, plasma membrane integrity, DNA integrity and acrosome integrity were evaluated. The 25 mM L-carnitine significantly improved sperm motility compared with a control group (p<0.05), although this was not significantly different among other concentrations. In conclusion, supplementation of 25 mM L-carnitine in freezing extender improves cauda epididymal spermatozoa motility. The effects of L-carnitine on the levels of oxidative stress during freezing and thawing remains to be examinedPublication Open Access Birth of kittens after the transfer of frozen-thawed embryos produced by intracytoplasmic sperm injection with spermatozoa collected from cryopreserved testicular tissue.(2012) T Tharasanit; S Buarpung; S Manee-In; C Thongkittidilok; N Tiptanavattana; P Comizzoli; M Techakumphu; Mahidol University. Faculty of Veterinary Science. Department of Clinical Science and Public Healthof blastomeres between frozen/thawed (242.5 ± 43.1) and fresh (320.2 ± 28.1) blastocysts. Three of seven cat recipients were pregnant and one pregnant cat delivered two healthy kittens. This is the first report of the birth of kittens after the transfer of frozenPublication Open Access Categorization of antimicrobial agents prescribed in the Veterinary Teaching Hospital in Thailand(2019) Arpron Leesombun; Sookruetai Boonmasawai; อาภรณ์ ลี้สมบุญ; สุขฤทัย บุญมาไสว; Mahidol University. Faculty of Veterinary Science. Department of Pre-clinic and Applied Animal ScienceAntimicrobial resistance (AMR) is critical situation for human and animal health worldwide. The inappropriate antimicrobial use is one of the risk factors of AMR. Intriguingly, an increase of antimicrobial resistance bacteria was recently evident in companion animals. Since such circumstance strongly implied the contribution of inappropriate antimicrobial use in veterinary clinics and hospitals to the problems, this study aimed to categorize and evaluate antimicrobials prescribed in the Veterinary Teaching Hospital in Thailand. From 6,200 animal patients, there were total 8,093 antimicrobial prescriptions for animal treatments. The antimicrobials were prescribed for parenteral administrations 3,346 prescriptions (41.3%) and oral administrations 4,747 prescriptions (58.7%), respectively. During 2012-2015, the most antimicrobial prescriptions were enrofloxacin (33.9%, P<0.001) with significantly difference from amoxicillin/clavulanate (24.1%) and doxycycline (15.2%). Enrofloxacin was highest prescribed in both parenteral and oral administrations (41.2% and 28.8%, respectively). Surgical procedure was topmost of parenteral antimicrobial prescriptions (16.5%, P<0.05), especially for neutering cases, followed with gastrointestinal system (14.6%) and dermatological system (9.5%). The most oral antimicrobial prescriptions were used for treatments of dermatological system (16.3%, P<0.05), gastro-intestinal system (11.1%) and surgical procedures (8.9%). These study revealed the pattern of antimicrobial use in companion animals to fulfill the associated picture with AMR problem in Thailand, and underline requirement of better solving plan based on one health approach with more concern of antimicrobial use in veterinary fields.Publication Open Access Nuclear scintigraphic examination in veterinary medicine(2015) Somkiat Huaijantug; Mahidol University. Faculty of Veterinary Science. Department of Clinical Sciences and Public HealthThe present review will discuss the information of nuclear scintigraphic examination in veterinary medicine. The main focus is on the introduction of nuclear medicine procedures in animal patients and describes conventional nuclear medicine (brain scintigraphy, bone scintigraphy, thyroid scintigraphy, inflammation and oncological scintigraphy). Single photon emission scintigraphy (SPECT) and positron emission tomography (PET) have recently been developed using for nuclear scintigraphic examination to provide functional and physiological imaging, the quantification of biochemical processes, molecular interactions, and neoplasias. Radiopharmaceuticals or radionuclides have a longer physical half-life allowing that need longer acquisition times used for specific investigation purposes. In veterinary medicine, scintigraphy can be the basis of a sensitive, specific and non-invasive diagnostic method, which information has been applied to support the diagnostic process and treatment planning for animal patients.